Boar sperm changes after sorting and encapsulation in barium alginate membranes

Spinaci, Marcella; Bucci, Diego; Chlapanidas, Theodora; Vallorani, Claudia; Perteghella, Sara; Communod, Ricardo; Vigo, D.; Tamanini, Carlo; Galeati, Giovanna; Faustini, M.; Torre, Maria Luisa

doi:10.1016/j.theriogenology.2013.05.018

Cited by 6 publications

(7 citation statements)

References 40 publications

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“…Moreover, although modifications on sperm tyrosine phosphorylation patterns immediately after sorting are scarce [37], overall percentage of sexed spermatozoa displaying an uncapacitated pattern after 72 hours storage was reduced [62]. This suggests a progressive modification toward a capacitationlike state of sexed spermatozoa during liquid storage as also confirmed by CTC results.…”

Section: Liquid Storage Of Sexed Boar Spermatozoasupporting

confidence: 59%

“…The evaluation of capacitation status, by CTC staining and immunolocalization of tyrosine phosphorylated proteins, revealed that the encapsulation process does not induce any further capacitation-like modification to sorted boar spermatozoa. In fact, no differences between liquid-stored and encapsulated sexed spermatozoa were recorded in the percentage of cells displaying different CTC and tyrosine phosphorylation patterns at 72 hours of storage [62]. Moreover, encapsulation during 72 hours of storage seems to protect sorted semen on the basis of percentage of sperm cells displaying the Hsp70 immunolocalization pattern typical of fresh semen being higher in the encapsulated sorted group (24.3%) compared with liquid-stored semen (2.8%) [62].…”

Section: Encapsulation Of Sex-sorted Boar Spermatozoamentioning

confidence: 81%

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Storage of sexed boar spermatozoa: Limits and perspectives

et al. 2016

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Despite the great potential application of sex-sorted spermatozoa in swine, the technology is not practiced in the pig industry because of technical factors and species-specific issues. The susceptibility of boar spermatozoa to stresses induced by the sorting procedure, the relative slowness of the sex-sorting process together with the high sperm numbers required for routine artificial insemination in pig are some of the main factors limiting the commercial application of this technology in pigs. This review briefly describes the damage to spermatozoa during sex sorting, focusing on an additional limiting factor: increased susceptibility of sexed boar spermatozoa to injuries induced by liquid storage and cryopreservation that, in turn, impairs sperm quality leading to unsatisfactory results in vivo. Strategies to extend the lifespan of sex-sorted boar spermatozoa and to improve their fertilizing ability after liquid storage or cryopreservation need to be implemented before this technology can be used in pig farms. In this regard, encapsulation in barium alginate membranes could be a promising technique to optimize the in vivo use of sexed boar spermatozoa, by protecting, targeting, and controlling the release of sperm into the female genital tract.

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Section: Liquid Storage Of Sexed Boar Spermatozoasupporting

confidence: 59%

Section: Encapsulation Of Sex-sorted Boar Spermatozoamentioning

confidence: 81%

Storage of sexed boar spermatozoa: Limits and perspectives

et al. 2016

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“…Some of these obstacles could be overcome by adopting a sperm encapsulation technique, which was previously demonstrated in bovine [13171836] and swine [78232930313334] species with promising results. Alginate encapsulation of sperm can limit damage to spermatozoa and allow prolonged sperm release; moreover, the bioadhesiveness of alginate can prevent semen backflow.…”

Section: Introductionmentioning

confidence: 99%

Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

et al. 2017

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The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. Although AI procedures are commonly used in bovine, use of AI has been limited in buffalo. In the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability of buffalo spermatozoa after AI. In this study, for the first time, the use of alginate encapsulation and cryopreservation of buffalo spermatozoa is described, and the same procedure was performed with Holstein Friesian (Bos taurus) semen. Results obtained from in vitro analyses indicate that the encapsulation process does not have detrimental effects (compared to controls) on quality parameters (membrane integrity, progressive motility, path average velocity) in either species. Similarly, there were no detrimental effects after cryopreservation in either species. The fertilizing potential of encapsulated and cryopreserved semen was evaluated after AI in 25 buffalo and 113 bovine females. Pregnancy rates were not affected in either species. The results of this study show proof of concept for the use of frozen semen controlled-release devices in buffalo.

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“…At the same time, encapsulation technology does not induce any additional damage to sorted spermatozoa (Spinaci et al. ,b). This experimental evidence suggests that encapsulation technology could enhance the in vivo fertilizing potential of sorted boar spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…), three different capacitation‐related parameters were investigated to evaluate the activation of encapsulated spermatozoa: chlortetracycline (CTC) staining, tyrosine phosphorylation and Hsp70 immunolocalization (Spinaci et al. ).…”

Section: Sex Sorting and Sperm Encapsulation: New Challenges In Boar mentioning

confidence: 99%

Sperm Encapsulation from 1985 to Date: Technology Evolution and New Challenges in Swine Reproduction

Perteghella

Vigani

Crivelli

et al. 2015

Reprod Domestic Animals

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Contents In the last 30 years, encapsulation technology has been applied to different species to minimize the loss of spermatozoa after artificial insemination. In particular, the vehiculation of boar sperm cells in barium alginate membrane has proved a valid strategy to reduce the risk of polyspermy and optimize in vivo fertilizing yields. Controlled release of male gametes into the female genital tract has reduced the minimum fertilizing dose of spermatozoa. Notwithstanding these results, encapsulation has not yet reached commercial application, largely due to the additional costs of production. However, encapsulation could be useful in advanced reproductive technology, such as sex sorting, to store sorted boar semen. The controlled release of flow cytometrically sorted spermatozoa could be a promising strategy to reduce the number of cells necessary for each insemination and hence allow the widescale use of sex sorting in this species.

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Boar sperm changes after sorting and encapsulation in barium alginate membranes

Cited by 6 publications

References 40 publications

Storage of sexed boar spermatozoa: Limits and perspectives

Storage of sexed boar spermatozoa: Limits and perspectives

Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

Sperm Encapsulation from 1985 to Date: Technology Evolution and New Challenges in Swine Reproduction

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