BNN-20, a synthetic microneurotrophin, strongly protects dopaminergic neurons in the “weaver” mouse, a genetic model of dopamine-denervation, acting through the TrkB neurotrophin receptor

Ablation of ErbB4 in parvalbumin‐positive interneurons inhibits adult hippocampal neurogenesis through down‐regulating BDNF/TrkB expression

“…The antibodies used are summarized in Table . Rabbit anti‐ErbB4 (1:1,000, Abcam, #ab32375, Cambridge, MA) antibody was purchased from Abcam; rabbit anti‐GAPDH (1:5,000; Cell Signaling Technology, #5174S, Danvers, MA) antibody was purchased from Cell Signaling Technology; mouse anti‐parvalbumin (PV, 1:2,000, Swant, #PV235, Bellinzona, Switzerland) antibody was purchased from Swant; goat anti‐Doublecortin (DCX, 1:100; Santa Cruz, #SC‐8066, CA) antibody was purchased from Santa Cruz; mouse anti‐BrdU (1:200; Roche, #11170376001, IN) antibody was purchased from Roche; rabbit anti‐NeuN (1:1,000, Millipore, #MABN140, Darmstadt, Germany) antibody was purchased from Millipore; rabbit anti‐BDNF (1:1,000; Abcam, #ab108319, Cambridge, MA) antibody (Einoch et al, ; Qian et al, ) was purchased from Abcam; rabbit anti‐BDNF (1:200; Alomone, #ANT010, Jerusalem, Israel) antibody (Fulmer et al, ; Wattananit et al, ) was purchased from Alomone; rabbit anti‐TrkB (1:1,000; Cell Signaling Technology, #4603S, Danvers, MA) antibody (Pandya et al, ; Qian et al, ) was purchased from Cell Signaling Technology; rabbit anti‐TrkB (1:200; GeneTex, #GTX54857, Irvine, CA) antibody (Botsakis et al, ) was purchased from GeneTex. These antibodies' specificity were confirmed using western blotting or/and immunohistochemistry (IHC) by the corresponding manufacturer and previous studies.…”

Effects of BNN27, a novel C17-spiroepoxy steroid derivative, on experimental retinal detachment-induced photoreceptor cell death

“…However, given the considerable clinical limitations of DHEA, due to its effects on the endocrine axis and its conversion to multiple androgen and estrogen metabolites 22 – 25 , several groups have synthesized a handful number of novel DHEA derivatives to mitigate this problem/effect 26 , 54 , 55 . Among them, to the best of our knowledge, only the spiro-analogs of DHEA (BNNs) have been tested and reported to have neuroprotective activity 26 , 27 , 29 , 30 , 56 , 57 . BNN27 can protect PC12 cells from serum deprivation-induced apoptosis 26 , 27 , can reduce TUNEL + cell death in superior cervical ganglia following NGF deprivation 27 and can also diminish caspase-3 mediated cell death in dorsal root ganglia of NGF null embryos 27 , in serum-deprived PC12 cells 27 and in cuprizone 29 - and diabetes 30 -induced apoptosis.…”

The beneficial role of the synthetic microneurotrophin BNN20 in a focal demyelination model

“…According to previous studies regarding BNN20, this compound is another DHEA derivative that binds and activates not only TrkA and p75 NTR like BNN27, but also TrkB. It was shown that BNN20 has a protective role regarding dopaminergic neurons, possibly, through TrkB in the weaver mouse mutant, which is a mouse model for Parkinson's disease (Botsakis et al, 2017). Moreover, recent studies showed that BNN20 has strong anti-inflammatory function, while being able to restore BDNF levels in this specific mouse model (Panagiotakopoulou et al, 2020).…”

“…In the present study, our aim was to elucidate the putative role of BNN20 in demyelination and remyelination, as a novel activator of both TrKA and TrkB, in contrast to the specific TrkA agonist, BNN27 (Botsakis et al., 2017; Panagiotakopoulou et al, 2020). As already known, there are plenty of models for studying demyelination and remyelination (Osorio‐Querejeta et al., 2017).…”