BnDGAT1s Function Similarly in Oil Deposition and Are Expressed with Uniform Patterns in Tissues of Brassica napus

Abstract

As an allotetraploid oilcrop, Brassica napus contains four duplicated Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) genes, which catalyze one of the rate-limiting steps in triacylglycerol (TAG) biosynthesis in plants. While all four BnDGAT1s have been expressed functionally in yeast, their expression patterns in different germplasms and tissues and also consequent contribution to seed oil accumulation in planta remain to be elucidated. In this study, the coding regions of the four BnDGAT1s were expressed in an Arabidopsis dgat1 mutant. All four BnDGAT1s showed similar effects on oil content and fatty acid composition, a result which is different from that observed in previous studies of their expression in yeast. Expression patterns of BnDGAT1s were analyzed in developing seeds of 34 B. napus inbred lines and in different tissues of 14 lines. Different expression patterns were observed for the four BnDGAT1s, which suggests that they express independently or randomly in different germplasm sources. Higher expression of BnDGAT1s was correlated with higher seed oil content lines. Tissue-specific analyses showed that the BnDGAT1s were expressed in a uniform pattern in different tissues. Our results suggest that it is important to maintain expression of the four BnDGAT1s for maximum return on oil content.

Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)

“…In this study, candidate RGs with one to four copies were evaluated together, but the copy numbers for them require validation once the whole genome of oat gets completed. Besides, different copies of duplicated genes usually display diverse tissue specificity or display various expression levels even in the same organ [54,55]. As shown in Table 1, different transcripts of TUV6 and UBC21 also showed two to seven-fold variation among their RPKM values in oat seed transcriptome.…”

Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)

“…In this study, candidate RGs with one to four copies were evaluated together, but the copy numbers for them require validation once the whole genome of oat gets completed. Besides, different copies of duplicated genes usually display diverse tissue speci city or display various expression levels even in the same organ [63,64]. As shown in Table 1, different transcripts of TUV6 and UBC21 also showed two to seven-fold variation among their RPKM values in oat seed transcriptome.…”

Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)

“…In this study, candidate RGs with one to four copies were evaluated together, but the copy numbers for them require validation once the whole genome of oat gets completed. Besides, different copies of duplicated genes usually display diverse tissue speci city or display various expression levels even in the same organ [54,55]. As shown in Table 1, different transcripts of TUV6 and UBC21 also showed two to seven-fold variation among their RPKM values in oat seed transcriptome.…”