BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus

Hu, Ran; Zhu, Meichen; Chen, Si; Li, Chengxiang; Zhang, Qianwei; Gao, Lei; Liu, Xueqin; Shen, Shuai; Fu, Fuyou; Xu, Xinhua; Liang, Ying; Liu, Liezhao; Lü, Kun; Yu, Hao; Li, Jiana; Qu, Cunmin

doi:10.1016/j.cj.2022.07.015

Cited by 8 publications

(7 citation statements)

References 67 publications

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“…The overexpression of the activator MaMYBPA in bananas induces the expression of the repressor MaMYBPR and these factors collectively regulate PA biosynthesis through competitive synergistic effects [53]. In Brassica napus, BnbHLH92a negatively regulates the synthesis of PAs and anthocyanins in the seed coat [54]. In this study, seven MYBs showed positive correlations with flavonol levels in rambutan peels and two bHLHs showed positive correlations with the PA content of rambutan peels.…”

Section: Discussionsupporting

confidence: 49%

Comparative Metabolomic and Transcriptomic Analysis Reveals That Variations in Flavonoids Determine the Colors of Different Rambutan Cultivars

Wang,

Zhu,

Yang

et al. 2024

Horticulturae

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Rambutan is a tropical tree and its fruit has several favorable characteristics. To understand how the color of the rambutan fruit peel develops, the transcriptome, flavonoid metabolome, and carotenoid metabolome data of two rambutan cultivars, ‘BY2’ and ‘BY7’, which show yellow and red peels at maturity, respectively, were comprehensively analyzed at three developmental stages. We identified 26 carotenoid components and 53 flavonoid components in these cultivars. Anthocyanins were the main component contributing to the red color of ‘BY7’ after reaching ripeness. The carotenoid content decreased sharply as the fruit matured. Hence, we speculated that flavonols were the main contributors to the yellow color of the ‘BY2’ peel. In total, 6805 differentially expressed genes were screened by transcriptome analysis; the majority of them were enriched in metabolic pathways and the biosynthesis of secondary metabolites. Weighted gene co-expression network analysis results revealed that in addition to MYB and bHLH, ERF, WRKY, MYB-related, and C3H were the main potential transcription factors regulating the color of the rambutan peel. In addition, we also identified 12 structural genes associated with flavonoid biosynthesis. The research findings shed light on the molecular mechanisms of color acquisition in rambutan fruit peels, laying the foundation for the quality control of rambutan and the cultivation of differently colored cultivars of rambutan.

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Section: Discussionsupporting

confidence: 49%

Comparative Metabolomic and Transcriptomic Analysis Reveals That Variations in Flavonoids Determine the Colors of Different Rambutan Cultivars

Wang,

Zhu,

Yang

et al. 2024

Horticulturae

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“…Transgenic expression of VdbHLH037 into Arabidopsis resulted in significant upregulation of structural genes involved in anthocyanin biosynthesis and high accumulation of anthocyanins. In strawberry fruits, FvbHLH9 positively regulates light-dependent anthocyanin biosynthesis by forming a heterodimer with HY [ 58 ], while in rapeseed, BnbHLH92a impinges on the biosynthesis of anthocyanins by interacting with the BnTTG1 [ 59 ]. In the present study, we found that the expression level of 13 IabHLH genes exhibited significant differences between purple-stemmed I. aquatica and green-stemmed I. aquatica .…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of bHLH Transcription Factors in Ipomoea aquatica and Its Response to Anthocyanin Biosynthesis

Liu

et al. 2023

IJMS

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The basic helix-loop-helix (bHLH) proteins compose one of the largest transcription factor (TF) families in plants, which play a vital role in regulating plant biological processes including growth and development, stress response, and secondary metabolite biosynthesis. Ipomoea aquatica is one of the most important nutrient-rich vegetables. Compared to the common green-stemmed I. aquatica, purple-stemmed I. aquatica has extremely high contents of anthocyanins. However, the information on bHLH genes in I. aquatica and their role in regulating anthocyanin accumulation is still unclear. In this study, we confirmed a total of 157 bHLH genes in the I. aquatica genome, which were classified into 23 subgroups according to their phylogenetic relationship with the bHLH of Arabidopsis thaliana (AtbHLH). Of these, 129 IabHLH genes were unevenly distributed across 15 chromosomes, while 28 IabHLH genes were spread on the scaffolds. Subcellular localization prediction revealed that most IabHLH proteins were localized in the nucleus, while some were in the chloroplast, extracellular space, and endomembrane system. Sequence analysis revealed conserved motif distribution and similar patterns of gene structure within IabHLH genes of the same subfamily. Analysis of gene duplication events indicated that DSD and WGD played a vital role in the IabHLH gene family expansion. Transcriptome analysis showed that the expression levels of 13 IabHLH genes were significantly different between the two varieties. Of these, the IabHLH027 had the highest expression fold change, and its expression level was dramatically higher in purple-stemmed I. aquatica than that in green-stemmed I. aquatica. All upregulated DEGs in purple-stemmed I. aquatica exhibited the same expression trends in both qRT-PCR and RNA-seq. Three downregulated genes including IabHLH142, IabHLH057, and IabHLH043 determined by RNA-seq had opposite expression trends of those detected by qRT-PCR. Analysis of the cis-acting elements in the promoter region of 13 differentially expressed genes indicated that light-responsive elements were the most, followed by phytohormone-responsive elements and stress-responsive elements, while plant growth and development-responsive elements were the least. Taken together, this work provides valuable clues for further exploring IabHLH function and facilitating the breeding of anthocyanin-rich functional varieties of I. aquatica.

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“…For example, our previous study showed that in NnTT8-overexpression Arabidopsis, lotus NnTT8 can interact with AtMYB114 to promote anthocyanin and PA accumulation in leaves and seeds, which may be due to activating transcript levels of ANS and UFGT genes [44]. However, some bHLH TFs, including BnbHLH92a in Brassica napus [47] and bHLH92 in sheepgrass [48], inhibited anthocyanin and PA accumulation. Hu et al reported that BnbHLH92a binds directly to the ANS gene promoter and represses its expression.…”

Section: The Different Functions Of Fttt8 In the Regulation Anthocyan...mentioning

confidence: 98%

Tartary Buckwheat (Fagopyrum tataricum) FtTT8 Inhibits Anthocyanin Biosynthesis and Promotes Proanthocyanidin Biosynthesis

Deng,

Wang,

Zhang

et al. 2023

IJMS

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Tartary buckwheat (Fagopyrum tataricum) is an important plant, utilized for both medicine and food. It has become a current research hotspot due to its rich content of flavonoids, which are beneficial for human health. Anthocyanins (ATs) and proanthocyanidins (PAs) are the two main kinds of flavonoid compounds in Tartary buckwheat, which participate in the pigmentation of some tissue as well as rendering resistance to many biotic and abiotic stresses. Additionally, Tartary buckwheat anthocyanins and PAs have many health benefits for humans and the plant itself. However, little is known about the regulation mechanism of the biosynthesis of anthocyanin and PA in Tartary buckwheat. In the present study, a bHLH transcription factor (TF) FtTT8 was characterized to be homologous with AtTT8 and phylogenetically close to bHLH proteins from other plant species. Subcellular location and yeast two-hybrid assays suggested that FtTT8 locates in the nucleus and plays a role as a transcription factor. Complementation analysis in Arabidopsis tt8 mutant showed that FtTT8 could not recover anthocyanin deficiency but could promote PAs accumulation. Overexpression of FtTT8 in red-flowering tobacco showed that FtTT8 inhibits anthocyanin biosynthesis and accelerates proanthocyanidin biosynthesis. QRT-PCR and yeast one-hybrid assay revealed that FtTT8 might bind to the promoter of NtUFGT and suppress its expression, while binding to the promoter of NtLAR and upregulating its expression in K326 tobacco. This displayed the bidirectional regulating function of FtTT8 that negatively regulates anthocyanin biosynthesis and positively regulates proanthocyanidin biosynthesis. The results provide new insights on TT8 in Tartary buckwheat, which is inconsistent with TT8 from other plant species, and FtTT8 might be a high-quality gene resource for Tartary buckwheat breeding.

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BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus

Cited by 8 publications

References 67 publications

Comparative Metabolomic and Transcriptomic Analysis Reveals That Variations in Flavonoids Determine the Colors of Different Rambutan Cultivars

Comparative Metabolomic and Transcriptomic Analysis Reveals That Variations in Flavonoids Determine the Colors of Different Rambutan Cultivars

Comprehensive Analysis of bHLH Transcription Factors in Ipomoea aquatica and Its Response to Anthocyanin Biosynthesis

Tartary Buckwheat (Fagopyrum tataricum) FtTT8 Inhibits Anthocyanin Biosynthesis and Promotes Proanthocyanidin Biosynthesis

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