BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation

Tao, Jianning; Kuliyev, Emin; Wang, Xi; Li, Xiuling; Wilanowski, Tomasz; Jane, Stephen M.; Mead, Paul E.; Cunningham, John M.

doi:10.1242/dev.01641

Cited by 52 publications

(58 citation statements)

References 83 publications

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“…They are implicated in epithelial morphogenesis, barrier formation and wound healing processes (Bray and Kafatos, 1991;Mace et al, 2005;Tao et al, 2005;Narasimha et al, 2008;Yu et al, 2009;Han et al, 2011;Gao et al, 2013). Grhl2 is one of three mouse homologs of Drosophila Grainyhead (Wilanowski et al, 2002) and is expressed in diverse embryonic epithelial tissues during development (Wilanowski et al, 2002;Auden et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

A Grhl2-dependent gene network controls trophoblast branching morphogenesis

et al. 2015

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Healthy placental development is essential for reproductive success; failure of the feto-maternal interface results in pre-eclampsia and intrauterine growth retardation. We found that grainyhead-like 2 (GRHL2), a CP2-type transcription factor, is highly expressed in chorionic trophoblast cells, including basal chorionic trophoblast (BCT) cells located at the chorioallantoic interface in murine placentas. Placentas from Grhl2-deficient mouse embryos displayed defects in BCT cell polarity and basement membrane integrity at the chorioallantoic interface, as well as a severe disruption of labyrinth branching morphogenesis. Selective Grhl2 inactivation only in epiblast-derived cells rescued all placental defects but phenocopied intraembryonic defects observed in global Grhl2 deficiency, implying the importance of Grhl2 activity in trophectoderm-derived cells. ChIP-seq identified 5282 GRHL2 binding sites in placental tissue. By integrating these data with placental gene expression profiles, we identified direct and indirect Grhl2 targets and found a marked enrichment of GRHL2 binding adjacent to genes downregulated in Grhl2(-/-) placentas, which encoded known regulators of placental development and epithelial morphogenesis. These genes included that encoding the serine protease inhibitor Kunitz type 1 (Spint1), which regulates BCT cell integrity and labyrinth formation. In human placenta, we found that human orthologs of murine GRHL2 and its targets displayed co-regulation and were expressed in trophoblast cells in a similar domain as in mouse placenta. Our data indicate that a conserved Grhl2-coordinated gene network controls trophoblast branching morphogenesis, thereby facilitating development of the site of feto-maternal exchange. This might have implications for syndromes related to placental dysfunction

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Section: Introductionmentioning

confidence: 99%

A Grhl2-dependent gene network controls trophoblast branching morphogenesis

et al. 2015

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“…It is accepted that the initial specification of Xenopus epidermis is controlled by a bone morphogenetic protein (BMP) activity gradient (Wilson and Hemmati-Brivanlou, 1995;Wilson et al, 1997;LaBonne and Bronner-Fraser, 1998;Marchant et al, 1998;Tribulo et al, 2003). A high BMP activity is able to promote epidermal fate (Wilson and HemmatiBrivanlou, 1995) by activating some transcription factors such as msx1, vent2, Dlx, AP2a, Xfoxi1a, and Grainyhead like-1 (Onichtchouk et al, 1996;Suzuki et al, 1997b;Luo et al, 2001Luo et al, , 2002Tao et al, 2005), which act both promoting epidermal development and inhibiting neural fate.…”

Section: Introductionmentioning

confidence: 99%

ΔNp63 is regulated by BMP4 signaling and is required for early epidermal development in Xenopus

Tríbulo

Barrionuevo

Agüero

et al. 2011

Developmental Dynamics

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Background: It has been established in several models that the p63 gene has an important role in the development of the epidermis and its derivatives. In Xenopus, only the DNp63 isoform of this gene has been cloned and its role during epidermal development remains unknown. Results: In this work, we showed that DNp63 is expressed in the nonneural ectoderm since the gastrula stage and that it is regulated by the bone morphogenetic protein 4 (BMP4) signaling pathway. Our in vivo and in vitro experiments demonstrated that DNp63 is required in the earliest inductive steps of epidermal development. The overexpression of DNp63 caused an increase in epidermal markers with a suppression of neural induction while the blocking of DNp63 led to the opposite results. Finally, we found that DNp63 acts as an anti-apoptotic gene, regulating the transcription of some apoptotic and anti-apoptotic factors. Conclusion: The results suggest that DNp63 is an essential gene in early epidermal specification under the control of BMP4. Developmental Dynamics 241:257-269,

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“…Grhls seem to have conserved functions controlling cell shape, cell growth, cell proliferation, and cell fate (7)(8)(9)(10)(11)(12)(13)(14). They maintain epithelial cell characteristics by regulating cell-cell junction genes including the desmosomal cadherin Desmoglein-1 (Dsg1) by Grhl1 and Claudin1 (Cldn1) and Occludin (Ocdn) by Grhl3.…”

mentioning

confidence: 99%

The Transcription Factors Grainyhead-like 2 and NK2-Homeobox 1 Form a Regulatory Loop That Coordinates Lung Epithelial Cell Morphogenesis and Differentiation

Varma

Cao

Tagne

et al. 2012

Journal of Biological Chemistry

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Background: Grhl2 regulates cell-junction gene transcription in several epithelia but has not been fully characterized in lungs. Results: In lung epithelial cells GRHL2 regulates cell-cell interaction genes, collective cell migration, and Nkx2-1 transcription. Conversely, NKX2-1 regulates transcription of Grhl2. Conclusion: A Grhl2-and Nkx2-1-positive transcriptional loop coordinates morphogenesis and differentiation of lung epithelial cells. Significance: This regulatory loop reinforces normal lung epithelial cell identity.

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BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation

Cited by 52 publications

References 83 publications

A Grhl2-dependent gene network controls trophoblast branching morphogenesis

A Grhl2-dependent gene network controls trophoblast branching morphogenesis

ΔNp63 is regulated by BMP4 signaling and is required for early epidermal development in Xenopus

The Transcription Factors Grainyhead-like 2 and NK2-Homeobox 1 Form a Regulatory Loop That Coordinates Lung Epithelial Cell Morphogenesis and Differentiation

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