BMP2-induced gene profiling in dental epithelial cell line

Miyoshi, Keiko; Nagata, Hiromitsu; Horiguchi, Taigo; Abe, Kaori; Wahyudi, Ivan Arie; Baba, Yoshinobu; Harada, Hidemitsu; Noma, Takafumi

doi:10.2152/jmi.55.216

Cited by 21 publications

(25 citation statements)

References 55 publications

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“…Bmp2 expression has been detected in epithelial and mesenchymal cells during tooth development [Aberg et al, 1997;Chen et al, 2008]. Bmp2 is able to induce ameloblast differentiation and enamel-related gene expression in vitro [Miyoshi et al, 2008]. These results indicate that Bmp2 is important for ameloblast differentiation and enamel formation.…”

Section: Introductionmentioning

confidence: 83%

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Abnormalities in the Enamel in Bmp2-Deficient Mice

Feng

Yang

Yuan

et al. 2011

Cells Tissues Organs

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Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (Bmp2) is essential for tooth formation. However, the role of Bmp2 during enamel formation remains unknown in vivo. In this study, the role of Bmp2 in the regulation of postnatal enamel formation was investigated via the conditional ablation of Bmp2 in enamel using the (Osx-Cre) mouse. Bmp2 gene ablation was confirmed by PCR analysis in Osx-Cre, Bmp2^flox/flox mice. Bmp2-null mice displayed a severe and profound tooth phenotype with asymmetric and open forked incisors. Microradiographs revealed broken incisor tips and dental pulp chamber exposure. The enamel layer of incisors and molars was thin with hypomineralization. Scanning electron microscopy analysis showed that the enamel surface was rough with chipping and the enamel lacked a typical prismatic architecture. These results demonstrate that Bmp2 is essential for enamel formation.

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Section: Introductionmentioning

confidence: 83%

“…Scale bar = 1 mm. [Miyoshi et al, 2008]. However, the role of Bmp2 in enamel formation in vivo has not been reported because mice with a traditionally targeted deletion of Bmp2 have embryonic lethality due to a failure of proamniotic canal closure or abnormal cardiac development, and they die at embryonic day 9.5 [Zhang and Bradley, 1996].…”

Section: Discussionmentioning

confidence: 99%

Abnormalities in the Enamel in Bmp2-Deficient Mice

Feng

Yang

Yuan

et al. 2011

Cells Tissues Organs

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“…If BMP2 is biologically involved in caries susceptibility, it is probably through the enamel development. In a published in vitro test, Bmp2 was able to induce ameloblast variation and enamel-related gene expression [Miyoshi et al, 2008]. A study with knockout mice for Bmp2 demonstrated that these animals have a thinner enamel and reduced mineral density [Feng et al, 2011].…”

Section: Discussionmentioning

confidence: 99%

<b><i>BMP2</i></b> Is Associated with Caries Experience in Primary Teeth

Romanos

Antunes²,

Lopes³

et al. 2015

Caries Res

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Bone morphogenetic proteins (BMPs) play an important role during the initial process of enamel development and therefore may play a role in caries susceptibility. The purpose of this study was to evaluate the association between the polymorphisms in the BMP2, BMP4 and BMP7 genes and their association with caries experience and primary enamel microhardness characteristics. DNA from buccal cells as well as clinical and demographic information from 1,731 subjects from three different data sets from Brazil were included. Polymorphisms in BMP2, BMP4 and BMP7 were analyzed by real-time polymerase chain reaction from genomic DNA. Association between caries experience, genotype, and allele distribution in both cohorts was evaluated using χ² and logistic regression analyses. In the family-based set, the association between caries experience and alleles was tested using the transmission disequilibrium test. In the Rio de Janeiro cohort, microhardness data on 108 exfoliated primary teeth before and after demineralization and remineralization challenges was included. Associations between microhardness values and genotype and allele distribution were evaluated using χ² and logistic regression analyses. Differences between caries experience and some risk factors were statistically significant. In the cohort from Nova Friburgo, BMP2 was associated with caries experience in primary dentition during logistic regression analysis (p = 0.023; OR = 2.58; 95% CI 1.13-5.86). There was no association between genotype and allele distribution for BMP polymorphisms and primary enamel microhardness alterations. Our result suggests that BMP2 may be involved in caries experience in primary dentition from a Nova Friburgo cohort.

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“…It was shown that BMP2 can accelerate amelogenesis 154 . Furthermore, there is a positive feedback mechanism linking some microRNAs, such as miR-200c and miR-203, and BMP signaling through regulating the expression of E-cadherin, amelogenin, and/or BMP antagonist BMPer 8 .…”

Section: Dental and Periodontal Tissue Engineeringmentioning

confidence: 99%

Wnt and BMP signaling crosstalk in regulating dental stem cells: Implications in dental tissue engineering

et al. 2016

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Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling. Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance, essential oral functions and the quality of life. Regenerative dentistry holds great promise in treating oral/dental disorders. The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells, along with the signaling mechanisms governing stem cell self-renewal and differentiation. In this review, we first summarize the biological characteristics of seven types of dental stem cells, including dental pulp stem cells, stem cells from apical papilla, stem cells from human exfoliated deciduous teeth, dental follicle precursor cells, periodontal ligament stem cells, alveolar bone-derived mesenchymal stem cells (MSCs), and MSCs from gingiva. We then focus on how these stem cells are regulated by bone morphogenetic protein (BMP) and/or Wnt signaling by examining the interplays between these pathways. Lastly, we analyze the current status of dental tissue engineering strategies that utilize oral/dental stem cells by harnessing the interplays between BMP and Wnt pathways. We also highlight the challenges that must be addressed before the dental stem cells may reach any clinical applications. Thus, we can expect to witness significant progresses to be made in regenerative dentistry in the coming decade.

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BMP2-induced gene profiling in dental epithelial cell line

Cited by 21 publications

References 55 publications

Abnormalities in the Enamel in Bmp2-Deficient Mice

Abnormalities in the Enamel in Bmp2-Deficient Mice

<b><i>BMP2</i></b> Is Associated with Caries Experience in Primary Teeth

Wnt and BMP signaling crosstalk in regulating dental stem cells: Implications in dental tissue engineering

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