BMP signaling is required for cell cleavage in preimplantation-mouse embryos

Mochel, Nabora Soledad Reyes de; Luong, Mui; Chiang, Michael; Javier, Anna L.; Luu, Elizabeth; Fujimori, Toshihiko; MacGregor, Grant R.; Cinquin, Olivier; Cho, Ken W.Y.

doi:10.1016/j.ydbio.2014.10.001

Cited by 35 publications

(43 citation statements)

References 62 publications

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“…We noted that only select ligands and receptors for the PDGF and BMP signaling pathways (including Pdgfa, Pdgfra, Bmp4 and Bmpr2 ) were significantly expressed. Despite the robust expression of components of these two pathways, our previous studies suggest that PDGFa-PDGFRa signaling is not involved in ICM cell fate specification (Artus et al, 2010), while others have demonstrated that loss or inhibition of BMP4/BMP7-BMPR2 signaling results in mild non-ICM-specific defects, predominantly in late stage (E4.5) blastocysts (Graham et al, 2014; Reyes de Mochel et al, 2015). …”

Section: Resultsmentioning

confidence: 81%

Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2

2017

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FGF4 is the key signal driving specification of primitive endoderm (PrE) versus pluripotent epiblast (EPI) within the inner cell mass (ICM) of the mouse blastocyst. To gain insight into the receptor(s) responding to FGF4 within ICM cells, we combined single-cell-resolution quantitative imaging with single-cell transcriptomics of wild-type and Fgf receptor (Fgfr) mutant embryos. Despite the PrE-specific expression of FGFR2, it is FGFR1, expressed by all ICM cells, which is critical for establishment of a PrE identity. Signaling through FGFR1 is also required to constrain levels of the pluripotency-associated factor NANOG in EPI cells. However, the activity of both receptors is required for lineage establishment within the ICM. Gene expression profiling of 534 single ICM cells identified distinct downstream targets associated with each receptor. These data lead us to propose a model whereby unique and additive activities of FGFR1 and FGFR2 within the ICM coordinate establishment of two distinct lineages.

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Section: Resultsmentioning

confidence: 81%

Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2

2017

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“…However, we cannot rule out that other major signalling pathways involved in patterning fields or groups of cells, such as Notch, Wnt, BMP, or EGF might also have an input 59 . In support of this, there are reports of Notch signalling involved in early mouse development [60][61][62] , as well as Wnt signalling 63 , BMP signalling 64,65 , EGF signalling 66 . In the light of our results, it will be important to revisit how these signalling pathways might be involved in cell fate decision in early blastocysts investigating how they affect cell position within the ICM.…”

Section: Spatial Pattern Of Nanog and Gata6 Expressing Cells In The Imentioning

confidence: 78%

Three-dimensional cell neighbourhood impacts differentiation in the inner mass cells of the mouse blastocyst

Fischer

Corujo-Simon

Lilao-Garzón

et al. 2017

Preprint

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Summary statementThree-dimensional cell neighbourhood, which includes fate marker levels, number of neighbouring cells and cell position, determines cell fate decision in early mouse embryos. AbstractDuring mammalian blastocyst development, inner cell mass (ICM) cells differentiate into epiblast (Epi) or primitive endoderm (PrE). These two fates are characterised by the transcription factors NANOG and GATA6, respectively. Here, we present quantitative three-dimensional single cell-based neighbourhood analyses to investigate the spatial distribution of NANOG and GATA6 expression in the ICM of the mouse blastocyst. The cell neighbourhood is characterised by the expression levels of the fate markers in the surrounding cells, together with the number of surrounding cells and cell position. We find that cell neighbourhoods are established in early blastocysts and different for cells expressing different levels of NANOG and GATA6. Highest NANOG expressing cells occupy specific positions within the ICM and are surrounded by 9 neighbours, while GATA6 expressing cells cluster according to their GATA6 levels. The analysis of mutants reveals that NANOG local neighbourhood is regulated by GATA6.All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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“…Several signaling pathways are thought to maintain the ICM lineages in the late blastocyst, including LIF/IL6/JAK/STAT and BMP pathways [71–73]. Interestingly, several early-expressed PE genes, such as Sox17 , Pdgfra , and Sox7 are not essential until after implantation [46, 67, 74].…”

Section: The Second Cell Fate Decision: the Latest Stagesmentioning

confidence: 99%

Cell signaling and transcription factors regulating cell fate during formation of the mouse blastocyst

2015

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The first cell fate decisions during mammalian development establish tissues essential for healthy pregnancy. The mouse has served as a valuable model for discovering pathways regulating the first cell fate decisions, because of the ease with which early embryos can be recovered and an arsenal of classical and emerging methods for manipulating gene expression. Here we summarize the major pathways that govern the first cell fate decisions in mouse development. This knowledge serves as a paradigm for exploring how emergent properties of a self-organizing system can dynamically regulate gene expression and cell fate plasticity. Moreover, it brings to light the processes that establish healthy pregnancy and embryonic stem (ES) cells. We also describe unsolved mysteries and new technologies that could help overcome experimental challenges in the field.

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BMP signaling is required for cell cleavage in preimplantation-mouse embryos

Cited by 35 publications

References 62 publications

Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2

Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2

Three-dimensional cell neighbourhood impacts differentiation in the inner mass cells of the mouse blastocyst

Cell signaling and transcription factors regulating cell fate during formation of the mouse blastocyst

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