BMP signaling-driven osteogenesis is critically dependent on Prdx-1 expression-mediated maintenance of chondrocyte prehypetrophy

Kumar, Yogesh; Biswas, Tathagata; Thacker, Gatha; Kanaujiya, Jitendra Kumar; Kumar, Sandeep; Shukla, Anukampa; Khan, Kainat; Sanyal, Sabyasachi; Chattopadhyay, Naibedya; Bandyopadhyay, Amitabha; Trivedi, Arun Kumar

doi:10.1016/j.freeradbiomed.2018.02.016

Cited by 17 publications

(13 citation statements)

References 39 publications

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“…Altered cell metabolism in chondrocytes has been associated with the production of ROS and stress response ( Gibson et al, 2008 ). Indeed, Peroxiredoxin-1 (PRDX1), a ROS scavenger ( Kumar et al, 2018 ), was significantly reduced ( p < 0.001). Heat shock protein 47 (HSP47), an endoplasmic collagen-binding stress protein, was increased in BMP7 ncko septa ( Figure 7D ).…”

Section: Resultsmentioning

confidence: 99%

“…Heat shock protein 47 (HSP47), an endoplasmic collagen-binding stress protein, was increased in BMP7 ncko septa ( Figure 7D ). ROS can induce chondrocyte hypertrophy ( Morita et al, 2007 ) in a BMP-dependant manner ( Kumar et al, 2018 ). Several indicators for chondrocyte hypertrophy were indeed increased [Matrix metalloproteinase-13 (MMP13) ( p < 0.001), RUNX2 ( p < 0.01), Collagen I (COL 1) ( p < 0.001)] ( Figure 7E ), while phosphorylated SMAD1/5/8 (pSMAD1/5/8) was overall reduced ( Figure 7F ).…”

Section: Resultsmentioning

confidence: 99%

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Nasal Septum Deviation as the Consequence of BMP-Controlled Changes to Cartilage Properties

Baddam

Young

Dunsmore

et al. 2021

Front. Cell Dev. Biol.

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The nasal septum cartilage is a specialized hyaline cartilage important for normal midfacial growth. Abnormal midfacial growth is associated with midfacial hypoplasia and nasal septum deviation (NSD). However, the underlying genetics and associated functional consequences of these two anomalies are poorly understood. We have previously shown that loss of Bone Morphogenetic Protein 7 (BMP7) from neural crest (BMP7ncko) leads to midfacial hypoplasia and subsequent septum deviation. In this study we elucidate the cellular and molecular abnormalities underlying NSD using comparative gene expression, quantitative proteomics, and immunofluorescence analysis. We show that reduced cartilage growth and septum deviation are associated with acquisition of elastic cartilage markers and share similarities with osteoarthritis (OA) of the knee. The genetic reduction of BMP2 in BMP7ncko mice was sufficient to rescue NSD and suppress elastic cartilage markers. To our knowledge this investigation provides the first genetic example of an in vivo cartilage fate switch showing that this is controlled by the relative balance of BMP2 and BMP7. Cellular and molecular changes similar between NSD and knee OA suggest a related etiology underlying these cartilage abnormalities.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Nasal Septum Deviation as the Consequence of BMP-Controlled Changes to Cartilage Properties

Baddam

Young

Dunsmore

et al. 2021

Front. Cell Dev. Biol.

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“…Moreover, PRDXs can also be divided into six subfamilies based on the structural information around the active sites including AhpC-Prx1, BCP-PrxQ, Tpx, Prx5, Prx6, and AhpE [ 6 ]. Currently, there are 6 known mammalian PRDX members, namely PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 [ 7 ]. The family members are involved in several biological processes, such as cell differentiation, metabolism [ 8 ], inflammation [ 9 ], cellular protection against reactive oxygen species (ROS) [ 10 ], embryonic development and cellular homeostasis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Integrative analysis the characterization of peroxiredoxins in pan-cancer

et al. 2021

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Background Peroxiredoxins (PRDXs) are an antioxidant enzymes protein family involved in several biological functions such as differentiation, cell growth. In addition, previous studies report that PRDXs play critical roles in the occurrence and development of carcinomas. However, few studies have conducted systematic analysis of PRDXs in cancers. Therefore, the present study sought to explore the molecular characteristics and potential clinical significance of PRDX family members in pan cancer and further validate the function of PRDX6 in bladder urothelial carcinoma (BLCA). Methods A comprehensive analysis of PRDXs in 33 types of cancer was performed based on the TCGA database. This involved an analysis of mRNA expression profiles, genetic alterations, methylation, prognostic values, potential biological pathways and target drugs. Moreover, both the gain and loss of function strategies were used to assess the importance and mechanism of PRDX6 in the cell cycle of BLCA. Result Analysis showed abnormal expression of PRDX1-6 in several types of cancer compared to normal tissues. Univariate Cox proportional hazard regression analysis showed that expression levels of PRDX1, PRDX4 and PRDX6 were mostly associated with poor survival of OS, DSS and PFI, and PRDX2 and PRDX3 with favorable survival. In addition, the expression of PRDX genes were positively correlated with CNV and negatively with methylation. Moreover, analysis based on PharmacoDB dataset showed that the augmented levels of PRDX1, PRDX3 and PRDX6 were significantly correlated with EGFR/VEGFR inhibitor drugs. Furthermore, knocking down of PRDX6 inhibited growth of cancer cells through the JAK2-STAT3 in bladder cell lines. Conclusions PRDXs are potential biomarkers and therapeutic targets for several carcinomas, especially for BLCA. In addition, PRDX6 could regulate proliferation of cancer cell via JAK2-STAT3 pathway and involve into the process of cell cycle in BLCA.

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“…According to the structural information around active sites, PRDXs are also divided into six subfamilies: AhpC-Prx1, BCP-PrxQ, Tpx, Prx5, Prx6, and AhpE 6 . At present, there are 6 known mammalian PRDX members, named PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 7 . The family members are involved in several biological processes, such as cell differentiation, metabolism 8 , inflammation 9 , cellular protection against reactive oxygen species (ROS) 10 , embryonic development and cellular homeostasis 11 .…”

Section: Introductionmentioning

confidence: 99%

Integrative Analysis the characterization of peroxiredoxins in pan-cancer

Gao

Meng

Yue

et al. 2020

Preprint

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Peroxiredoxins (PRDXs) are antioxidant enzymes protein family members that involves the process of several biological functions, such as differentiation, cell growth. Considerable evidence demonstrates that PRDXs play critical roles in the occurrence and development of carcinomas. However, a systematic analysis of PRDXs in cancers is deficiency. Therefore, we perform a comprehensive analysis of PRDXs in 33 cancer types including mRNA expression profiles, genetic alterations, methylation, prognostic values, potential biological pathways and target drugs. Moreover, we validated that PRDX6 could regulate cancer cell proliferation via JAK2-STAT3 pathway and involve into the process of cell cycle in bladder cancer.

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BMP signaling-driven osteogenesis is critically dependent on Prdx-1 expression-mediated maintenance of chondrocyte prehypetrophy

Cited by 17 publications

References 39 publications

Nasal Septum Deviation as the Consequence of BMP-Controlled Changes to Cartilage Properties

Nasal Septum Deviation as the Consequence of BMP-Controlled Changes to Cartilage Properties

Integrative analysis the characterization of peroxiredoxins in pan-cancer

Integrative Analysis the characterization of peroxiredoxins in pan-cancer

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