BMP-6 inhibits MMP-9 expression by regulating heme oxygenase-1 in MCF-7 breast cancer cells

Hu, Fang; Guo, Shaocong; Mi, Dong; Shen, Wenwen; Zhang, Jie; Qiao, Ying; Zhu, Tianhui; Yang, Shuang

doi:10.1007/s00432-010-0963-z

Cited by 29 publications

(21 citation statements)

References 47 publications

(39 reference statements)

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“…Moreover, this finding was consistent with prior studies that reported that HO-1 mediated cell invasion and migration in A459 cells [12] and in other types of tumor cells [61]. However, several reports have suggested that HO-1 overexpression suppresses angiogenesis and tumor invasion [39,][62]. This discrepancy may be due to the dual effect of HO-1 on tumor invasion depending on the specific type of cancer or other unknown factors.…”

Section: Discussionsupporting

confidence: 80%

“…In this study, we reported that HG treatment induced an increase in CD147 and MMP-9 expression was and that this increase was decreased by HO-1 knockdown in A459 cells. This finding suggested that CD147 and MMP-9 were associated with HO-1 expression levels, thereby lending credence to previous reports indicating the involvement of HO-1 in CD147 and MMP-9 expression in A459 cells [12] and in other tumor cells [13,39]. Based on our results, we propose that HG increases metastasis-associated protein (CD147 and MMP-9) expression by up-regulating HO-1 expression, which may promote tumor metastasis.…”

Section: Discussionsupporting

confidence: 70%

“…CD147 is a highly glycosylated transmembrane protein that regulates many protein families and that affects tumor invasiveness and metastasis [12,36,37]. MMP-9 and CD147 expression is associated with the malignant potential of pancreatic cancer cells [38], NSCLC cells [12] bladder cancer cells [13] and breast cancer cells [39]. Several lines of evidence have indicated that HO-1 induces tumor invasiveness and metastasis by regulating these metastasis-associated proteins [18,40].…”

Section: Introductionmentioning

confidence: 99%

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High Glucose Promotes Tumor Invasion and Increases Metastasis-Associated Protein Expression in Human Lung Epithelial Cells by Upregulating Heme Oxygenase-1 via Reactive Oxygen Species or the TGF-β1/PI3K/Akt Signaling Pathway

Kang

Kong

et al. 2015

Cell Physiol Biochem

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Background: Growing evidence indicates that heme oxygenase-1 (HO-1) is up-regulated in malignancies and subsequently alters tumor aggressiveness and various cancer-related factors, such as high glucose (HG) levels. HO-1 expression can be induced when glucose concentrations are above 25 mM; however, the role of HO-1 in lung cancer patients with diabetes remains unknown. Therefore, in this study we investigated the promotion of tumor cell invasion and the expression of metastasis-associated proteins by inducing the up-regulation of HO-1 expression by HG treatment in A549 human lung epithelial cells. Methods: The expression of HO-1and metastasis-associated protein expression was explored by western blot analysis. HO-1 enzymatic activity, reactive oxygen species (ROS) production and TGF-β₁ production were examined by ELISA. Invasiveness was analyzed using a Transwell chamber. Results: HG treatment of A549 cells induced an increase in HO-1 expression, which was mediated by the HG-induced generation of reactive oxygen species (ROS) and transforming growth factor-β1 (TGF-β₁) in a concentration- and time-dependent manner. Following the increase in HO-1 expression, the enzymatic activity of HO-1 also increased in HG-treated cells. Pretreatment with N-acetyl-L-cysteine (NAC) or with phosphatidylinositol 3-kinase (PI3K)/Akt inhibitors attenuated the HG-induced increase in HO-1 expression. HG treatment of A549 cells enhanced the invasion potential of these cells, as shown with a Transwell assay, and increased metastasis-associated protein expression. However, HO-1 siRNA transfection significantly decreased these capabilities. Conclusion: this study is the first to demonstrate that HG treatment of A549 human lung epithelial cells promotes tumor cell invasion and increases metastasis-associated protein expression by up-regulating HO-1 expression via ROS or the TGF-β₁/PI3K/Akt signaling pathway.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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High Glucose Promotes Tumor Invasion and Increases Metastasis-Associated Protein Expression in Human Lung Epithelial Cells by Upregulating Heme Oxygenase-1 via Reactive Oxygen Species or the TGF-β1/PI3K/Akt Signaling Pathway

Kang

Kong

et al. 2015

Cell Physiol Biochem

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“…MMPs were frequently shown to be upregulated in several types of cancer, and their expression was often associated with poor patient prognosis (25)(26)(27)(28)(29). The difference in the expression of MMPs in breast cancer cell lines of different tumorigenicity correlates with the biological behavior of these cells: more malignant cells expressed more MMPs than the less malignant ones (30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

The Association of Matrix Metalloproteinase-1 Promoter Polymorphisms with Breast Cancer

Hsiao

Liu

Shih

et al. 2018

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Abstract. Background For many years, breast cancer has been the most common malignancy and the leading cause of female cancer mortality all over the world (1). According to the most updated report about burden of disease trends, cancers overall have increased by 34% during 199-2015 while breast cancer related deaths globally have increased to 45%, a much higher level than average overall cancers (1). In Taiwan, breast cancer has the highest incidence and is ranked as the fourth leading cause of mortality among Taiwanese women (2). From the viewpoint of epidemiology, the risk factors of breast cancer in Taiwan included high caloric intake, highfat diets, early menarche, late menopause, obesity, high levels of stress, and exposure to environmental pollutants (3). Since, the prevalence and mortality rates are both very high in Taiwan and the world, to figure out feasible molecular markers for early detection and prognosis prediction of breast cancer, especially the subtype of triple negative breast cancer (TNBC), are in urgent need.The matrix metalloproteinases (MMPs), which are also called matrixins, are a group of enzymes involved in tumor progression such as proliferation, invasion and metastasis (4, 5). In the literature, there are a few papers indicating that some MMP polymorphic genotypes, especially those 487

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“…In contrast, they inhibit cell proliferation of some cancers such as breast and myeloma [38,47,48]. BMP-6 also inhibits breast cancer cell migration and invasion [51,52]. In contrast, it increases migration and invasion of prostate cancers [53].…”

Section: Bone Morphogenetic Proteins and Cancermentioning

confidence: 99%

An Update of Bone Morphogenetic Proteins as Biomarker and Therapy for Cancer

Rahman¹

2015

J Carcinog Mutagen

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BMP-6 inhibits MMP-9 expression by regulating heme oxygenase-1 in MCF-7 breast cancer cells

Abstract: These observations suggest a novel role of BMP-6/HO-1 cascade to relieve breast cancer metastasis by regulating the secretion of growth factors in tumor microenvironment.

Cited by 29 publications

References 47 publications

High Glucose Promotes Tumor Invasion and Increases Metastasis-Associated Protein Expression in Human Lung Epithelial Cells by Upregulating Heme Oxygenase-1 via Reactive Oxygen Species or the TGF-β1/PI3K/Akt Signaling Pathway

High Glucose Promotes Tumor Invasion and Increases Metastasis-Associated Protein Expression in Human Lung Epithelial Cells by Upregulating Heme Oxygenase-1 via Reactive Oxygen Species or the TGF-β1/PI3K/Akt Signaling Pathway

The Association of Matrix Metalloproteinase-1 Promoter Polymorphisms with Breast Cancer

An Update of Bone Morphogenetic Proteins as Biomarker and Therapy for Cancer

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