BMP-2 promotes chondrogenesis of rat adipose-derived stem cells by using a lentiviral system

Abstract

ABSTRACT. Osteoporosis poses a major public health threat in aging societies. Adipose-derived stem cells (ADSCs) are multipotent adult stem cells that have the ability to yield mesenchymal stem cells, and have the potential to undergo osteogenesis and bone regeneration. Bone morphogenetic proteins (BMPs) have been demonstrated to upregulate bone gene expression after mechanical injury and to improve bone injury repair. This study aimed to produce BMP-2 expression in ADSCs by using lentiviral vectors. Subcutaneous adipose tissue from 4-week-old male Sprague-Dawley rats was used. Oil red O staining was used to detect adipocyte formation from ADSCs. Induction of ADSC osteogenesis was confirmed with Alizarin red S staining. The recombinant lenti-hBMP-2/ BMP-2 promotes chondrogenesis of rat adipose neo was constructed to infect ADSCs, BMP-2 expression was measured by immunoblotting analysis, and cellular alkaline phosphatase levels were examined. We found that >70% of ADSC cells could be induced to differentiate into osteocytes or adipocytes. Under osteogenic induction, ADSCs showed increased intracellular calcium deposition, the formation of calcium tubercles, and the disappearance of cellular structures in calcium tubercles. After infection of ADSCs by lenti-hBMP-2/neo, BMP-2 was expressed after doxycycline induction. We, thus, conclude that ADSCs maintain vigorous growth ex vivo and possess stem celllike properties. When infected with lenti-hBMP-2/neo, ADSCs can be induced to promote BMP-2 expression.

The Potential of Menstrual Blood-Derived Mesenchymal Stem Cells for Cartilage Repair and Regeneration: Novel Aspects

“…Nevertheless, during MenSC differentiation, they detected a significant increase in the expression level of Collagen 9A1 and the transcription factor SOX9, suggesting that these cells positively respond to chondrogenic induction. Moreover, considering different growth factor influence on chondrogenic differentiation, it is important to note that there are studies suggesting that TGF- β 3 does not always induce chondrogenesis in such cells as ADSC and BMMSCs, where BMP-2 was shown to act as a major chondrogenic differentiation inducer in ADSC [65, 66], and the combination of TGF- β 1, GDF-5, and BMP-2 stimulated robust chondrogenic response in BMMSCs [67]. These observations may lead to the development of new strategies for novel chondrogenic differentiation protocols for MenSCs, which will include additional factors that these cells may require.…”

Effect of BMP-2 Adherent to Resorbable Sutures on Cartilage Repair: A Rat Model of Xyphoid Process

“…Three days after surgery, the anastomoses with IGF-I coated sutures showed better anastomotic healing (histology appearance) and greater capacity to withstand biomechanical stress (busting pressure) than the control groups [ 23 ]. As an FDA-approved biologic, BMP-2 appears promising in promoting chondrogenesis and cartilage repair at pre-clinical setting [ 27 , 28 , 29 ]. The current study with BMP-2 coating on the resorbable sutures provides evidence to further support the theory that growth factors can be successfully introduced to a local tissue environment by coating suture material.…”

Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis

“…ADSCs were isolated from the inguinal fat pads of 3-week-old male Lewis rats according to previously reported methods. 24 After being isolated, ADSCs were maintained in complete growth medium (Dulbecco's modified Eagle's medium [DMEM], Invitrogen) containing L-glutamine supplemented with 10% fetal bovine serum (FBS) and 1%…”

“…was negatively correlated in patients with clinical OP. Previous reports have shown that rat primary adipose stem cells have pluripotent differentiation potential and can be induced to differentiate into adipocytes and chondrocytes 24. Bone remodeling is the basis of the process of bone repair or osteogenesis in many skeletal diseases.…”