BMP-2 can inhibit bone healing: Bone-chamber study in rabbits

Jeppsson, Charlotte; Aspenberg, Per

doi:10.3109/17453679608997762

Cited by 35 publications

(20 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experiments in spontaneously healing bone defects are rare, but increased strength of osteotomies treated with BMP-2 in rabbits has recently been reported (Turek et al 1997). We found inhibition of bone and tissue formation when introducing BMP-2 into a spontaneously healing skeletal defect in a titanium chamber in rabbits (Jeppsson and Aspenberg 1996). As these findings conflicted with previous experiments concerning BMPs, we then varied the experimental conditions to find a reason for this inhibition.…”

Section: Charlotte Jeppsson' Mathias Bostrom2 and Per Aspenberglcontrasting

confidence: 54%

“…In previous experiments, we found inhibition of bone formation when BMP-2 was placed in the bone harvest chamber (Jeppsson and Aspenberg 1996). These findings were probably not caused by too high a BMP concentration, because the inhibition was still found when the BMP-2 dose was lowered to less than one hundredth of the dose suggested for clinical use.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Intraosseous BMP implants in rabbits Inhibitory effect on bone formation

Jeppsson

Bostrom

Aspenberg

1999

Acta Orthopaedica Scandinavica

Self Cite

View full text Add to dashboard Cite

Section: Charlotte Jeppsson' Mathias Bostrom2 and Per Aspenberglcontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 89%

Intraosseous BMP implants in rabbits Inhibitory effect on bone formation

Jeppsson

Bostrom

Aspenberg

1999

Acta Orthopaedica Scandinavica

Self Cite

View full text Add to dashboard Cite

“…One must also keep in mind that BMP-2 has the potency to induce or modulate apoptosis, 35,36 and that in vivo application of high doses of BMP-2 may inhibit bone formation. 37 These examples stress the importance of methods for testing BMP-2 in conjunction with implant materials.…”

Section: Discussionmentioning

confidence: 99%

A reporter‐cell assay for the detection of BMP‐2 immobilized on porous and nonporous materials

Wiemann¹,

Jennissen

Rumpf

et al. 2002

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Human recombinant bone morphogenetic protein-2 (rhBMP-2) immobilized on the surface of metal implants can facilitate osseointegration. Here, we describe a cell reporter assay useful for quantifying small amounts of immobilized rhBMP-2 on various materials. The peptide was dotted and heat-fixed on titanium, 316L stainless steel, nitrocellulose, or glass, and its distribution was monitored by in situ biotinylation followed by detection with the avidin-biotin method. Bioactivity of rhBMP-2 was demonstrated by means of a confluent layer of osteoblastic MC3T3-E1 cells that evenly covered rhBMP-2-free and rhBMP-2-loaded surface areas, as shown with epifluorescence microscopy of calcein acetoxymethyl (AM)-loaded cells. Expression of osteocalcin, fibronectin, actin, and vimentin increased where cells were located on rhBMP-2 dotted areas, but the signal:noise ratio was too low to bioassay the peptide. However, local pronounced expression of alkaline phosphatase was used to quantify BMP-2 in the range of 5-80 ng/dot by means of a cytochemical color reaction for alkaline phosphatase and image analysis of resulting dots. The lower detection limit was in the order nitrocellulose > glass > titanium > 316L steel. We conclude that the cell reporter assay is useful to assess biological activity of rhBMP-2 even after immobilization on three-dimensional implant materials.

show abstract

“…The marked cells are still abundant directly inside the transition zone (c). The control tissue was negative for LacZ stained cells, as shown here at 2 weeks (d) ize in the clinical setting and single high-dose applications can be even detrimental to the healing process [12].…”

Section: Discussionmentioning

confidence: 98%

Gene transfer to the tendon-bone insertion site

Lattermann

Zelle

Whalen

et al. 2004

Knee Surg Sports Traumatol Arthrosc

View full text Add to dashboard Cite

This study investigated whether gene transfer to the tendon-bone insertion site is possible during early tendon-transplant healing using viral vectors. In addition, we evaluated the optimal gene delivery technique for an in vivo adenoviral gene transfer to a tendon-bone insertion site in a bone tunnel. Twenty-six rabbits underwent a bilateral transfer of the flexor digitorum longus tendon into a bone canal in the calcaneus. The animals were divided into two groups. The first group (n=18) received a direct injection of an adenoviral vector carrying the luciferase marker gene into the tendon on the left side, while on the right side the adenoviral vector was first injected into the bone trough and the tendon was later inserted into the trough. The analysis of this experiment showed that over a 4-week period a higher luciferase activity was achieved using the bone trough immersion technique. In the second group (n=8) we therefore used the qualitative marker virus (Ad/-LacZ) with the bone trough immersion technique in order to show the site of gene expression. The histological analysis of this experiment demonstrated the presence of beta-galactosidase positive cells within the tendon-bone interface over a 4-week period. Therefore we showed in the first part of this study that the bone canal provides a more efficient target for direct adenoviral gene delivery than the tendon. In the second part of the study we demonstrated the feasibility of the bone trough immersion technique since sustained gene expression within the tendon-bone interface was obtained for up to 4 weeks. This study has shown the feasibility of gene delivery to the tendon-bone interface and provides the basis for the application adenoviral delivery of growth factor genes to the tendon-bone insertion site.

show abstract

BMP-2 can inhibit bone healing: Bone-chamber study in rabbits

Cited by 35 publications

References 8 publications

Intraosseous BMP implants in rabbits Inhibitory effect on bone formation

Intraosseous BMP implants in rabbits Inhibitory effect on bone formation

A reporter‐cell assay for the detection of BMP‐2 immobilized on porous and nonporous materials

Gene transfer to the tendon-bone insertion site

Contact Info

Product

Resources

About