bmo-miR-2739 and the novel microRNA miR-167 coordinately regulate the expression of the vitellogenin receptor in <i>Bombyx mori</i> oogenesis

Chen, Enxiang; Chen, Zhiwei; Li, Shenglong; Xing, Dongxu; Guo, Huizhen; Liu, Jianqiu; Ji, Xiaocun; Lin, Ying; Liu, Shiping; Xia, Qingyou

doi:10.1242/dev.183723

Cited by 21 publications

(22 citation statements)

References 50 publications

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“…Notably, two miRNAs, miR-2739 and miR-167 bind directly to the 3 ′ UTR of B. mori VgR mRNA and coordinately inhibit VgR expression. Overexpression of either miR-2739 or miR-167 using the piggyBac system blocked Vg deposition into oocytes, whereas application of miR-2739 or miR-167 antagomiR resulted in increase of VgR levels and uptake of Vg by oocytes (Chen et al, 2020).…”

Section: Vgr-mediated Vg Endocytosismentioning

confidence: 97%

Regulatory Mechanisms of Vitellogenesis in Insects

Yang

et al. 2021

Front. Cell Dev. Biol.

118

109

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Vitellogenesis is pre-requisite to insect egg production and embryonic development after oviposition. During insect vitellogenesis, the yolk protein precursor vitellogenin (Vg) is mainly synthesized in the fat body, transported by the hemolymph through the intercellular spaces (known as patency) in the follicular epithelium to reach the membrane of maturing oocytes, and sequestered into the maturing oocytes via receptor-mediated endocytosis. Insect vitellogenesis is governed by two critical hormones, the sesquiterpenoid juvenile hormone (JH) and the ecdysteriod 20-hydroxyecdysone (20E). JH acts as the principal gonadotropic hormone to stimulate vitellogenesis in basal hemimetabolous and most holometabolous insects. 20E is critical for vitellogenesis in some hymenopterans, lepidopterans and dipterans. Furthermore, microRNA (miRNA) and nutritional (amino acid/Target of Rapamycin and insulin) pathways interplay with JH and 20E signaling cascades to control insect vitellogenesis. Revealing the regulatory mechanisms underlying insect vitellogenesis is critical for understanding insect reproduction and helpful for developing new strategies of insect pest control. Here, we outline the recent research progress in the molecular action of gonadotropic JH and 20E along with the role of miRNA and nutritional sensor in regulating insect vitellogenesis. We highlight the advancements in the regulatory mechanisms of insect vitellogenesis by the coordination of hormone, miRNA and nutritional signaling pathways.

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Section: Vgr-mediated Vg Endocytosismentioning

confidence: 97%

Regulatory Mechanisms of Vitellogenesis in Insects

Yang

et al. 2021

Front. Cell Dev. Biol.

118

109

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“…However, how LmDDX3 regulates the LmVgR expression needs to be further investigated. Very recently, two miRNA was found to mediate VgR expression in Bombyx mori (Chen et al, 2020). Whether this is conserved in locust remains still a detailed investigation needs to be performed.…”

Section: Discussionmentioning

confidence: 99%

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Wang

Deng

et al. 2021

Arch Insect Biochem Physiol

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DDX3 represents a well‐defined subfamily of DEAD‐box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown that LmDDX3, the ortholog of DDX3 in Locusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of LmDDX3 results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterized LmDDX3 expression in female adult locust and analyzed its function in oocyte development. LmDDX3 was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development. LmDDX3 RNAi led to a reduced vitellogenin (Vg) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation of vitellogenin receptor (VgR) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role for LmDDX3 in regulating the transcription of Vg and VgR, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.

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“…According to the dosage used in previous papers, for each silkworm larva, 12.5 μg of Bmo-miR-282-5p-agomir (282-agomir) was injected into the haemolymph at C1 stage when a dim spot on the dorsal side of the spiracle becomes a triangular-shaped semitransparent area (Chen et al, 2020;Jiang et al, 2013;Kiguchi & Agui, 1981). A total of 23 animals were injected for each miRNA agomir.…”

Section: Dual-luciferase Assaymentioning

confidence: 99%

The miR‐282‐5p regulates larval moulting process by targeting chitinase 5 in Bombyx mori

Wang

Zhang

Lai

et al. 2021

Insect Molecular Biology

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Moulting is critical for growth, development and survival in insects. As the main components of cuticle, dynamic change of chitin is consistent with the moulting process. Chitinase is the main enzyme to mediate chitin metabolism in the old cuticle. To avoid over-degrading chitin from the new cuticle, the expression of chitinase must be precisely regulated. In this study, we performed microRNA-sequencing to investigate expression change of microRNAs in silkworm epidermis during the moulting process. A comparative microRNA transcriptomic analysis from different moulting stages and 20-hydroxyecdysone (20E) treatment identified bmo-miR-282-5p as a candidate. By the bioinformatic analysis, chitinase 5 (BmCht5) was predicted to be a target of bmo-miR-282-5p. Meanwhile, a temporal expression analysis revealed that BmCht5 only expressed at moulting D3 stage, whereas bmo-miR-282-5p showed a converse pattern, in which its transcript signal disappeared at this time point. Furthermore, a luciferase assay and agomir treatment demonstrated that bmo-miR-282-5p suppressed transcript of BmCht5 in vivo. As a result, injection of 282-5p agomir triggered 40% death due to moulting failure. In addition, RNA interference (RNAi)-mediated silencing of BmCht5 caused 30% developmental defect. Taken together, our data demonstrate the coordinated regulation of chitinase 5 by conserved miR-282-5p, and the 20E signalling pathway is essential for the normal moulting process in the domesticated silkworm.

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bmo-miR-2739 and the novel microRNA miR-167 coordinately regulate the expression of the vitellogenin receptor in Bombyx mori oogenesis

Cited by 21 publications

References 50 publications

Regulatory Mechanisms of Vitellogenesis in Insects

Regulatory Mechanisms of Vitellogenesis in Insects

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

The miR‐282‐5p regulates larval moulting process by targeting chitinase 5 in Bombyx mori

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