“…Primary myofibroblast cultures were treated in vitro with IL-15, IL-15/IL-15Rα complex, and/or TGF-β for 48 h in the presence of monensin (2.0 mM; Thermo Fisher Scientific, Illkirch, France, #00-4505) for the last 18 h. Cells were detached with Accutase (Sigma-Aldrich, St. Quentin Fallavier, France, A6964), and intracellular expression of MCP-1 and fibronectin was assessed by flow cytometry as previously described [ 63 ]. Briefly, after fixation and permeabilization using cytofix/cytoperm solution (BD Biosciences, Franklin Lakes, NY, USA), cells were stained with antibodies against MCP-1 (Thermo Fisher Scientific, Illkirch, France, #MA5-17040) or fibronectin (Abcam, Cambridge, UK, Ab2413) for 45 min in the dark at 4 °C, washed with 1× Perm/Wash solution, and labeled with a phycoerythrin (PE)-conjugated secondary antibody for 45 min in the dark at 4 °C.…”