BM88/CEND1 coordinates cell cycle exit and differentiation of neuronal precursors

Politis, Panagiotis K.; Makri, Georgia; Thomaidou, Dimitra; Geissen, Markus; Rohrer, Hermann; Matsas, Rebecca

doi:10.1073/pnas.0610973104

Cited by 62 publications

(110 citation statements)

References 35 publications

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“…Forced expression of BM88/Cend1 in the early neural tube led neural progenitors to overcome the anti-differentiating barrier of lateral inhibition by downregulating Notch1 and its downstream effector protein, Hes5, thus giving birth to large numbers of neighbouring post-mitotic neurons in the otherwise Notch1 expressing area of the VZ. 12 As a result, misexpression of BM88/Cend1 in VZ precursors was sufficient to prematurely initiate and successfully conclude their differentiation program producing terminally differentiated neurons within the VZ. These ectopic neurons expressed characteristic molecular markers of the neuronal cytoskeleton, such as βIII-tubulin and neurofilament proteins as well as appropriate transcription factors and neurotransmitters.…”

Section: Bm88/cend1 Is a Dual Function Molecule Promoting Cell Cycle mentioning

confidence: 99%

“…12,42 Our overall analysis suggested that BM88/Cend1 exerts dual action on neuron generation: first, it strongly increases the probability that neuronal progenitors exit the cell cycle and start differentiating and, second, it drives postmitotic cells to become terminally differentiated neurons. The result was precocious and overt neuronal differentiation.…”

Section: Bm88/cend1 Is a Dual Function Molecule Promoting Cell Cycle mentioning

confidence: 99%

“…40 The apparently low expression of BM88/Cend1 in neuronal progenitors is readily elevated upon precursor to neuron switch and can be induced by retinoic acid, 12,27,41,42 or other differentiation promoting agents, such as the histone deacetylase inhibitor trichostatin A (our unpublished observations). In the embryonic mouse and chick spinal cord a medio-lateral and dorso-ventral gradient of BM88/Cend1 expression is apparent, with lower BM88/Cend1 levels in the neural stem/ progenitor cell population of the VZ and higher in the differentiated cells of the MZ in the medio-lateral axis.…”

Section: The In Vivo Expression Pattern Of Bm88/cend1 Suggests a Rolementioning

confidence: 99%

“…A number of key factors regulating cell cycle progression have been implicated in cell fate determination and differentiation of neuronal precursors, while specification-and/or differentiation-inducing molecules are beginning to emerge as cell cycle regulators. [7][8][9][10][11][12] Early in development, a population of competent ectodermal cells are committed to neural fate to form the neural plate, and later produce the neural tube, of which the anterior part generates the forebrain and the posterior part the spinal cord. The spinal cord develops from a small number of highly plastic neural stem/progenitor cells that proliferate, acquire regional identities and generate a progressively restricted repertoire of cell types, first neurons and later oligodendroglial and astroglial cells.…”

Section: Introductionmentioning

confidence: 99%

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Coordination of cell cycle exit and differentiation of neuronal progenitors

Politis

Thomaidou²,

Matsas³

2008

Cell Cycle

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During development, co-ordinate regulation of cell cycle exit and differentiation of neuronal precursors is essential for generation of appropriate number of neurons and proper wiring of neuronal circuits. Recent studies have identified some of the molecules implicated in the regulation of these cellular events, but the complex machinery that orchestrates these processes into a coherent developmental program remains unclear. BM88/Cend1 is a neuronal protein associated in vivo with terminal neuron-generating divisions, marking the exit of proliferative cells from the cell cycle. Genetic studies in neural cell lines, neural stem/progenitor cells using the neurosphere system and in the developing chicken neural tube in vivo have shown that BM88/Cend1 is a dual function molecule co-ordinating cell cycle exit and differentiation of neuronal progenitors. These studies have thus shed light on a molecular determinant that participates, along with other known and possibly still unknown regulators, in the complex processes by which a progenitor cell becomes a mature neuron.

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Section: Bm88/cend1 Is a Dual Function Molecule Promoting Cell Cycle mentioning

confidence: 99%

Section: Bm88/cend1 Is a Dual Function Molecule Promoting Cell Cycle mentioning

confidence: 99%

Section: The In Vivo Expression Pattern Of Bm88/cend1 Suggests a Rolementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Coordination of cell cycle exit and differentiation of neuronal progenitors

Politis

Thomaidou²,

Matsas³

2008

Cell Cycle

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“…is first expressed in RGCs from E16, as faint staining is BM88, which was isolated from porcine brain and is identical to C38, is reported to down-regulate proliferation and enhance neuronal differentiation of neural progenitor cells derived from embryonic spinal cord as well as adult neural precursors in the subventricular zone 13,14) . However, in these cells, cell fate determination and the following neuronal maturation are a continuing phenomenon and it is difficult to distinguish between them.…”

Section: -4 Developmental Expression Patterns Of C38 In the Retina mentioning

confidence: 99%

C38 is a Neuron Specific Mitochondrial Protein that Controls Neuronal Development

Wakabayashi

Kimura²,

Yamada³

2010

J Kansai Med Univ

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Abstract:The retina has a well-organized cellular structure and belongs to the central nervous system. The retina has been used as a model of the central nervous system, especially neuronal degeneration and axonal regeneration. Monoclonal C38 antibody has been used as a molecular marker of retinal ganglion cells, but the molecular function of its antigen has not been clarified. A recent study has demonstrated that C38 is a neuron-specific mitochondrial protein that enhances neuronal maturation. This review outlines the functions of C38, as investigated through a retina model.

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Profiling of mouse synaptosome proteome and phosphoproteome by IEF

et al. 2010

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Synapses play important roles in neurotransmission and neuroplasticity. For an in-depth analysis of the synaptic proteome and phosphoproteome, synaptosomal proteins from whole mouse brain were analyzed by IEF and MS resulting in the largest synaptosome proteome described to date, with 2980 unique proteins identified with two or more peptides. At the same time, 118 synaptosomal phosphoproteins were identified, eight of which are reported for the first time as phosphorylated. Expression of selected proteins in synaptosomes was investigated by Western blot. We demonstrate that IEF is a powerful method to interrogate complex samples such as brain tissue both at the proteome and the phosphoproteome level without the need of additional enrichment for phosphoproteins. The detailed synaptoproteome data set reported here will help to elucidate the molecular complexity of the synapse and contribute to our understanding of synaptic systems biology in health and disease.

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BM88/CEND1 coordinates cell cycle exit and differentiation of neuronal precursors

Cited by 62 publications

References 35 publications

Coordination of cell cycle exit and differentiation of neuronal progenitors

Coordination of cell cycle exit and differentiation of neuronal progenitors

C38 is a Neuron Specific Mitochondrial Protein that Controls Neuronal Development

Profiling of mouse synaptosome proteome and phosphoproteome by IEF

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