Blueberry latent virus: An amalgam of the Partitiviridae and Totiviridae

Martín, Robert R.; Zhou, Jing; Tzanetakis, Ioannis E.

doi:10.1016/j.virusres.2010.09.020

Cited by 77 publications

(89 citation statements)

References 30 publications

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“…S2; total length, 671 aa) were concatenated in order to construct a maximumlikelihood phylogeny using PhyML (LG model) and branch support was measured by approximate likekihood ratio tests (aLRT; Guindon et al, 2010). We used monopartite plant viruses belonging to the proposed family Amalgaviridae as an outgroup (Martin et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Additional heritable virus in the parasitic wasp Leptopilina boulardi: prevalence, transmission and phenotypic effects

Martinez

Lepetit

Ravallec

et al. 2016

Journal of General Virology

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Parasitoid wasps can be found in association with heritable viruses. Although some viruses have been shown to profoundly affect the biology and evolution of parasitoid wasps, the genetic and phenotypic diversity of parasitoid-associated viruses remains largely unexplored. We previously discovered a behaviour-manipulating DNA virus in the parasitoid wasp Leptopilina boulardi. In this species, which lays its eggs inside Drosophila larvae, Leptopilina boulardi filamentous virus (LbFV) forces the females to lay their eggs in already parasitized Drosophila larvae. This behavioural manipulation increases the chances for the horizontal transmission of the virus. Here, we describe in the same parasitoid species another virus, which we propose to call Leptopilina boulardi toti-like virus (LbTV). This doublestranded RNA virus is highly prevalent in insect laboratory lines as well as in parasitoids caught in the field. In some cases, LbTV was found in coinfection with LbFV, but did not affect the behaviour of the wasp. Instead we found that the presence of LbTV correlates with an increase in the number of offspring, mostly due to increased survival of parasitoid larvae. LbTV is vertically transmitted mostly through the maternal lineage even if frequent paternal transmission also occurs. Unlike LbFV, LbTV is not horizontally transmitted. Its genome encodes a putative RNA-dependent RNA polymerase (RdRp) showing similarities with RdRps of Totiviridae. These results underline the high incidence and diversity of inherited viruses in parasitoids as well as their potential impact on the phenotype of their hosts.

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Section: Methodsmentioning

confidence: 99%

Additional heritable virus in the parasitic wasp Leptopilina boulardi: prevalence, transmission and phenotypic effects

Martinez

Lepetit

Ravallec

et al. 2016

Journal of General Virology

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“…Blueberry latent virus (BBLV), found during an etiological study of blueberry fruit drop disease, was associated with latent infection of highbush blueberry trees (Martin et al 2006(Martin et al , 2011. The BBLV genome is a single dsRNA with two partially overlapping open reading frames (designated as ORF1 and ORF2 from the 5 0 end of the genome) similar to those of totiviruses, which consist of fungal or protozoal viruses (Wickner et al 2005).…”

mentioning

confidence: 99%

“…The BBLV genome is a single dsRNA with two partially overlapping open reading frames (designated as ORF1 and ORF2 from the 5 0 end of the genome) similar to those of totiviruses, which consist of fungal or protozoal viruses (Wickner et al 2005). In comparison to RNAdependent RNA polymerase domain (RdRp domain) encoded by the ORF2, BBLV is closely related to but distinct from partitiviruses whose genomes have two segments each per coat protein (CP) and RdRp (Martin et al 2011). Recently, BBLV-like viruses, which include Southern tomato virus, Vicia cryptic virus-M and rhododendron virus A were also found in tomato, broad bean and rhododendron, respectively (Liu and Chen 2009;Sabanadzovic et al 2009Sabanadzovic et al , 2010.…”

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confidence: 99%

Histochemical detection of Blueberry latent virus in highbush blueberry plant

et al. 2011

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Blueberry latent virus (BBLV) was detected in 27 of 95 asymptomatic highbush blueberry trees in a blueberry field in Japan. In situ hybridization showed that the viral RNAs were detected in the palisade mesophyll, spongy mesophyll and vascular bundle of leaves. In western blot analysis, an antibody to the viral protein encoded by open reading frame 1 (ORF1) reacted with a 48-kDa protein specific for blueberry trees in which BBLV was detected. Immunogold electron microscopy revealed that amorphous bodies in the cytoplasm of blueberry cells were labeled with antibodies to the ORF1 protein.

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“…Amalgaviruses are persistent, appear to be cryptic, share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae (Martin et al, 2011). In this report, we present the characterization of two novel tentative members of the Amalgaviridae family and the Amalgavirus genus, the first viruses associated to T. kok-saghyz.…”

Section: Introductionmentioning

confidence: 95%

“…http://dx.doi.org/10.1101/229443 doi: bioRxiv preprint first posted online Dec. 5, 2017; Novel Amalgaviruses of Rubber Dandelion was reported for Blueberry latent virus, when 35 diverse cultivars were assessed and over 99% sequence identity among isolates was observed (Martin et al, 2011). Additionally, SNP were predicted by implementing the FreeBayes tool (Garrinson & Marth, 2012), and 259 variants were identified among the CDS of RdLV1 & RdLV2 (Figure 2.E); 78.37% of the polymorphisms involved the 3 rd position of the predicted codon, suggesting a tentative constraint to avoid amino acid changes and thus maintain structure and functional domains of the respective viruses.…”

Section: Cc-by-nc-nd 40 International License Peer-reviewed) Is the mentioning

confidence: 99%

Molecular Identification and Characterization of Two Rubber Dandelion Amalgaviruses

Debat¹,

Luo

Iaffaldano

et al. 2017

Preprint

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SummaryThe Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the detected virus sequences suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop.

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Blueberry latent virus: An amalgam of the Partitiviridae and Totiviridae

Cited by 77 publications

References 30 publications

Additional heritable virus in the parasitic wasp Leptopilina boulardi: prevalence, transmission and phenotypic effects

Additional heritable virus in the parasitic wasp Leptopilina boulardi: prevalence, transmission and phenotypic effects

Histochemical detection of Blueberry latent virus in highbush blueberry plant

Molecular Identification and Characterization of Two Rubber Dandelion Amalgaviruses

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