Blueberries in Romania: past, present and future perspective

Asănică, A.; Bădescu, Alexandru; Bădescu, Codruţa

doi:10.17660/actahortic.2017.1180.39

Cited by 4 publications

(1 citation statement)

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“…In light of the growing demand for blueberries worldwide, the everchanging demand by consumers for different fruit colors, taste, flavors, together with climate change and the need for varieties resistant to biotic and abiotic stress, the pressure for creating new varieties resistant to environmental factors that will also satisfy the customers' demands is ever-increasing [13][14][15][16]. One way to hasten the process of creating novel varieties is to employ molecular biology techniques such as the next-generation sequencing (NGS) of whole genomes [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Looking in the Scaffold 22 Hotspot for Differentially Regulated Genes Genomic Sequence Variation in Romanian Blueberry Cultivars

Mihai,

Bădulescu,

Asănică

et al. 2024

Horticulturae

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Since its domestication about a century ago in North America, highbush blueberry (Vaccinium corymbosum L.) has gained appreciation by consumers worldwide, and the demand for new blueberry varieties is increasing. Whole-genome resequencing can help plant breeders to decrease the time needed to create novel varieties by identifying novel genes linked to fruit-quality traits. The present study analyzed the genetic variability of eight V. corymbosum genotypes, seven Romanian varieties (‘Prod’, ‘Vital’, ‘Azur’, ‘Simultan’, ‘Delicia’, ‘Compact’, and ‘Safir’), and the American variety, ‘Bluecrop’. The analysis of the first ~10 Mb from scaffold 22, a hotspot of genomic variation, in the above-mentioned varieties revealed multiple differences in 11 upregulated and 50 downregulated genes involved in fruit growth and development. Of these differentially regulated genes, two upregulated and five downregulated genes were fully covered by at least 1× coverage depth by sequencing. The genes’ sequence analysis confirmed the high genetic variability of the region, with most of the genes presenting numerous SNPs and some InDels, and indicated that an attempted 10× medium-coverage depth of sequencing for V. corymbosum varieties yields useful preliminary data for use in breeding programs.

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