Blue light irradiation and its beneficial effect on Dupuytren’s fibroblasts

Borgschulze, Annika; Sahlender, Benita; Lögters, Tim; Windolf, Joachim; Grotheer, Vera

doi:10.1371/journal.pone.0209833

Cited by 11 publications

(14 citation statements)

References 31 publications

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“…This finding was rather interesting, because as described in aforementioned DD studies, the already existing ROS burden was associated with the pathogenesis of DD [14,15]. Our previous study could also determine an elevated ROS amount in DD fibroblasts and that blue light treatment with a wavelength of (λ = 453 nm), further gains ROS formation [20]. This raises the question of how blue light irradiation affects the intracellular ROS level and controls DD pathology and thus its therapy.…”

Section: Introductionsupporting

confidence: 62%

“…Before being released for delivery the irradiance of the LED-device was validated by the manufacturer using an integrating (Ulbricht) sphere. The power density was 38 mW/ cm 2 , at a distance of 8 cm, the time of exposure was 16.7 min, according to 40 J/cm 2 , a nontoxic dose [20]. The exact irradiation time was evaluated using the precise hand radiometer RM 21 (Dr. Gröbel UV-Elektronik GmbH (Ettlingen, Germany).…”

Section: Blue Light Irradiationmentioning

confidence: 99%

“…Intracellular ROS concentrations under physiological conditions are balanced in between their rates of production and protective antioxidant cellular mechanism. ROS homeostasis is mediated by different enzymes as NADPH Oxidase (NOX) and Xanthine Oxidase (XO), producing superoxide (O 2 -), the most reactive oxygen radical [19] Our previous in vitro study demonstrates that initial elevated and activated α-SMA expression in DD fibroblasts can be significantly inhibited by blue light irradiation (λ = 453 nm, 40 J/ cm 2 , 38 mW/cm 2 ) [20]. With a longer the blue light treatment, α-SMA protein expression decreases.…”

Section: Introductionmentioning

confidence: 99%

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Illuminating the effect of beneficial blue light and ROS-modulating enzymes in Dupuytren’s disease

et al. 2021

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Dupuytren’s disease (DD) is a fibroproliferative disorder of the palmar aponeurosis, which is characterized by a compound myofibrogenesis and evidenced by an increased expression of α-smooth muscle actin (α-SMA). In Dupuytren’s tissue, higher levels of reactive oxygen species (ROS) are documented, stimulating the proliferation and differentiation of myofibroblasts. Our preliminary study demonstrates that α-SMA-expression is significantly inhibited by blue light irradiation in DD. The objective of this study was to investigate the beneficial effect of blue light irradiation and to elucidate the influence of ROS on myofibrogenesis in the pathogenesis of DD. Therefore, an in-vitro model of human DD fibroblasts was used. DD fibroblasts and control fibroblasts isolated from carpal tunnel syndrome (CTS) were daily irradiated with 40 J/cm2 (λ = 453 nm, 38 mW/cm2). Protein expression of ROS-modulating enzymes (Catalase, NOX4, SOD1, MnSOD) and α-SMA were determined, and additionally analysed after a pharmacological inhibition of the TGF-β1-signaling with SB431542. Furthermore, the protein expression of α-SMA as surrogate parameter for myofibrogenesis was evaluated after applying different concentrations of long-lasting ROS. It could be determined that the beneficial blue light irradiation, which inhibited myofibrogenesis, is mediated by a significant inhibition of catalase protein expression. This effect should be accompanied with an increased intracellular ROS level. Proof of evidence was an H2O2-application on DD fibroblasts, also leading to a decreased myofibrogenesis. Furthermore, it could be demonstrated that endogenous MnSOD was significantly downregulated in resting DD fibroblasts. If DD fibroblasts were treated with the pharmacological inhibitor SB431542, myofibrogenesis was inhibited, but MnSOD expression was simultaneously elevated, which ought to affect ROS level by raising intracellular H2O2 amount. Blue light irradiation as well as the pharmacological action of SB431542 in consequence mediates their beneficial effect on disturbed myofibrogenesis in DD by further increasing ROS level. The present study demonstrates the importance of intracellular ROS homeostasis in DD and illuminates the beneficial effect of blue light as a promising therapy option for DD.

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Section: Introductionsupporting

confidence: 62%

Section: Blue Light Irradiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Illuminating the effect of beneficial blue light and ROS-modulating enzymes in Dupuytren’s disease

et al. 2021

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“…Cell Counting Kit-8 (CCK-8) was used to measure cellular metabolic activity [20,21], while Sulforhodamine B-based assay (SRB) was used to evaluate cell proliferation [22][23][24]. The CCK-8 uses WST-8, which produces a water-soluble formazan dye according to the mitochondrial dehydrogenase activity.…”

Section: Cell Counting Kit-8 and Sulforhodamine B Based Assaysmentioning

confidence: 99%

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

et al. 2021

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In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here, we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts from healthy skin and keratinocytes. Different light doses (3.43, 6.87, 13.7, 20.6, 30.9 and 41.2 J/cm2) were used to treat the cells, evidencing inhibitory and stimulatory effects following a biphasic dose behavior. Electrophysiology was used to investigate the effects on membrane currents: healthy fibroblasts and keratinocytes showed no significant differences between treated and not treated cells. Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation: a significant decrease in peak intensity of healthy fibroblast was evidenced, while it is less pronounced in keratinocytes. In conclusion, we observed that the blue LED light can be used to modulate metabolism and proliferation of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

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“…Cell Counting Kit-8 (CCK-8) was used to measure cellular metabolic activity, [22,23] while Sulforhodamine B based assay (SRB) was used to evaluate cell proliferation [24][25][26]. Both CCK-8 and SRB were purchased from Sigma-Aldrich (Milan, Italy) and used in according to manufacturer instructions.…”

Section: Cell Counting Kit-8 and Sulforhodamine B Based Assaysmentioning

confidence: 99%

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Rossi

Magni

Tatini

et al. 2020

Preprint

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In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts and keratinocytes. Different light doses were used to treat the cells, evidencing inhibitory and stimulatory effects. Electrophysiology was used to investigate the effects on membrane currents, while Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation. In conclusion, we observed that the blue LED light can be used to modulate the activity of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

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Blue light irradiation and its beneficial effect on Dupuytren’s fibroblasts

Cited by 11 publications

References 31 publications

Illuminating the effect of beneficial blue light and ROS-modulating enzymes in Dupuytren’s disease

Illuminating the effect of beneficial blue light and ROS-modulating enzymes in Dupuytren’s disease

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

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