Blue Light Inhibits the Growth of B16 Melanoma Cells

Ohara, Masaru; Kawashima, Yuzo; Katoh, Osamu; Watanabe, Haruo

doi:10.1111/j.1349-7006.2002.tb01290.x

Cited by 49 publications

(45 citation statements)

References 20 publications

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“…This was carried out to avoid the interaction between possible variables in the medium, such as color or soluble proteins, with the effect of blue light on the cells [7]. Our results contradict a previous paper in which the authors report that the exposure of B16 melanoma cells to blue light inhibited their growth [24]. A possible source of error for their setup is the inclusion of culture medium in the plate wells during the exposure to blue light, thereby introducing extra variables into the experiment.…”

Section: Discussioncontrasting

confidence: 84%

The effect of blue light exposure and use of intraocular lenses on human uveal melanoma cell lines

et al. 2006

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Little is known about the effect of blue light on inducing melanocytic malignant transformation. We chose to investigate the effect of blue light (475 nm wavelength) on the proliferation rates of uveal melanoma cells. In addition, we tested two different intraocular lenses to determine the possible effects of ultraviolet absorbing and blue light filtering intraocular lenses on the changes in proliferation. Four human uveal melanoma cell lines (92.1, MKT-BR, OCM-1, SP6.5) were exposed to blue light with and without the presence of ultraviolet absorbing and blue light filtering intraocular lenses. Cells covered by aluminum foil were used as a control. The proliferation rate of the cells compared with the control was then assessed using the Sulforhodamine-B proliferation assay. Cells exposed to blue light showed a statistically significant (P<0.05) increase in proliferation. Those exposed to blue light through a standard ultraviolet absorbing intraocular lens showed a smaller increase in proliferation, whereas those exposed with a blue light filtering intraocular lens showed no increase in proliferation than the control in all four cell lines. The exposure of cells to blue light led to an increase in proliferation in all cell lines compared with the control. The use of blue light filtering intraocular lenses abolished these increases in proliferation in the four cell lines. These results indicate that blue light filtering intraocular lenses may have a protective effect on the proliferation rates of uveal melanoma cells exposed to blue light.

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Section: Discussioncontrasting

confidence: 84%

The effect of blue light exposure and use of intraocular lenses on human uveal melanoma cell lines

et al. 2006

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“…14 Animal and human studies also have demonstrated the potential utility of blue light for cancer therapy. 15,16 The mechanisms by which blue light affects cells remain unclear, but roles for cellular 14,27,28 These data support a hypothesis that blue light induced-ROS mediate, at least in part, the suppression of mitochondrial function. However, the roles of intra-and extracellular sources of the ROS in causing blue light effects, a possible role for riboflavin or flavins in vitro in generating ROS, and the degree to which these two sources interact to cause cellular responses are unclear.…”

Section: Introductionsupporting

confidence: 71%

“…15,19,23 This hypothesis predicts that riboflavin (or other extracellular components) produce ROS that migrate intracellularly and affect cell responses. The current results demonstrate that peroxide concentrations generated in…”

Section: External Peroxide Vs Intracellular Rosmentioning

confidence: 99%

Intra‐ and extracellular reactive oxygen species generated by blue light

Omata

Lewis

Rotenberg

et al. 2006

J Biomedical Materials Res

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Acknowledgement of Financial AssistanceThe authors thank the Medical College of Georgia and Ministry of Funding in Japan for seed funds to do this work. 2 SYNOPSISBlue light from dental photopolymerization devices has significant biological effects on cells.These effects may alter normal cell function of tissues exposed during placement of oral restorations, but, recent data suggest that some light-induced effects also may be therapeutically useful, for example in the treatment of epithelial cancers. Reactive oxygen species (ROS) appear to mediate blue light effects in cells, but the sources of ROS (intra-vs. extra-cellular) and their respective roles in the cellular response to blue light are not known. In the current study, we tested the hypothesis that intra-and extra-cellular sources of blue lightgenerated ROS synergize to depress mitochondrial function. Methods: Normal human epidermal keratinocytes (NHEK) and oral squamous cell carcinoma (OSC2) cells were exposed to blue light (380-500 nm; 5-60 J/cm 2 ) from a dental photopolymerization source (quartztungsten-halogen, 550 mW/cm 2 ). Light was applied in cell-culture media or balanced salt solutions with or without cells present. Intracellular ROS levels were estimated using the dihydrofluorescein diacetate (DFDA) assay; extracellular ROS levels were estimated using the leucocrystal violet assay. Cell response was estimated using the MTT mitochondrial activity assay. Results: Blue light increased intracellular ROS equally in both NHEK and OSC2. Blue light also increased ROS levels in cell-free MEM or salt solutions, and riboflavin supplements increased ROS formation. Extracellularly applied ROS rapidly (50-400 μM, < 1 min) increased intracellular ROS levels, which were higher and longer-lived in NHEK than OSC2. The type of cell-culture medium significantly affected the ability of blue light to suppress cellular mitochondrial activity; the greatest suppression was observed in DMEM-containing or NHEK media. Collectively, the data support our hypothesis that intra-and extracellularly generated ROS synergize to affect cellular mitochondrial suppression of tumor cells in response to blue light. However, the identity of blue light targets that mediate these changes remain unclear.These data support additional investigations into the risks of coincident exposure of tissues to blue light during material polymerization of restorative materials, and possible therapeutic 14,27,28 These data support a hypothesis that blue light induced-ROS mediate, at least in part, the suppression of mitochondrial function. However, the roles of intra-and extracellular sources of the ROS in causing blue light effects, a possible role for riboflavin or flavins in vitro in generating ROS, and the degree to which these two sources interact to cause cellular responses are unclear. In the current study, we show that both intra-and extra-cellular ROS are generated by blue light in epithelial cultures, and that both mediate blue light induced-mitochondrial responses. Our data support furthe...

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“…The effect of blue light in cells has being studied by many groups [28][29][30][31][32] and it has been reported that blue light suppressed the growth (time-dependent) and also inhibited melanin synthesis in B16 melanoma [24,26]. The same effect did not occur for green and red light.…”

Section: Introductionmentioning

confidence: 91%

“…The same effect did not occur for green and red light. These authors suggested that the effect of blue light could be due to the inhibition of DNA synthesis and cell division [28,[30][31][32].…”

Section: Introductionmentioning

confidence: 97%

Photodynamic therapy for Photogem® and Photofrin® using different light wavelengths in 375 human melanoma cells

et al. 2007

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Melanoma is the most lethal of all cutaneous malignancies and additional treatment paradigms are needed. We explored the activity of PDT on human A-375 melanoma cells in vitro with different light wavelengths using porphyrin photosensitizers. The effect of red, green, and blue light in the cytotoxicity of these cells was measured, and the results suggest that there are no differences in the photodynamic activity of Photofrin and Photogem when irradiated with the blue, green and red light, if the effect of the molar absorption coefficient and the effect of light penetration in the tissue are taking into account. We report the ID 10 (irradiation dose responsible for killing 90% of cells) for Photofrin and Photogem irradiated in the three wavelengths. The fact that melanoma cells have problems with the absorption of the light due to poor penetration of the light administrated in PDT (red light: 630 nm) suggests that the melanoma cells can be irradiated with blue and green light producing the same cytotoxic result, only with an adjustment of the light dose. This may offer a means to improve clinical PDT for patients with this diagnosis.

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Blue Light Inhibits the Growth of B16 Melanoma Cells

Cited by 49 publications

References 20 publications

The effect of blue light exposure and use of intraocular lenses on human uveal melanoma cell lines

The effect of blue light exposure and use of intraocular lenses on human uveal melanoma cell lines

Intra‐ and extracellular reactive oxygen species generated by blue light

Photodynamic therapy for Photogem® and Photofrin® using different light wavelengths in 375 human melanoma cells

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