Blue light induced reactive oxygen species from flavin mononucleotide and flavin adenine dinucleotide on lethality of HeLa cells

Differential response of human dermal fibroblast subpopulations to visible and near‐infrared light: Potential of photobiomodulation for addressing cutaneous conditions

“…The results of our study also agrees with previously published data on the involvement of ROS as part of the immediate effect of light on cells . It has been demonstrated that exposure to short visible wavelength light induced ROS in various models and could result in DNA damage in human epithelial cells for example .…”

“…High quantities of ROS have been shown to be generated by short visible wavelength light acting on flavoproteins and nitrosated proteins [13][14][15][16] and this has been proposed to be the first point of light interaction with cells. We now know that ROS are involved in the molecular reactions generated by visible light, both at short but also at long wavelengths, in range of different cell types including but not limited to HeLa cells [18], adipose-derived stem cells [16], human skin fibroblasts and keratinocytes [19,20], and others [18,[21][22][23][24].…”

Identification of Flavin Mononucleotide as a Cell‐Active Artificial N6‐Methyladenosine RNA Demethylase

“…Direct conversion of the m 6 A oligonucleotide (blue line) yielded two new peaks (turquoise line), and eventually to one peak (yellow line). [13] Notably,t he total transcriptomic RNAi solated from the FMN/irradiation-treated HeLa cells showed ad ramatic decrease of the m 6 Al evel, with a p-value of less than 0.01, compared to that of untreated cells ( Figure 1F). Interestingly,t he other new peak that appeared during the demethylation was identified as the same sequence with f 6 A ( Figure S4), which strongly indicates that f 6 Ai sa no xidative intermediate,while no other reaction occurs in the process.T o further validate the generality of the demethylation, two 9-mer ssRNAoligonucleotides with m 6 Aincorporated in the internal and 5'-positions (5'-CUGGm 6 ACUGG-3' and 5'm 6 ACUGACUAG-3')were synthesized.…”

Identification of Flavin Mononucleotide as a Cell‐Active Artificial N6‐Methyladenosine RNA Demethylase

“…Thev iability assay of HeLa cells with above mentioned treatments indicated that both FMN and light irradiation had minor effect on cell growth (Figure S5). [13] Notably,t he total transcriptomic RNAi solated from the FMN/irradiation-treated HeLa cells showed ad ramatic decrease of the m 6 Al evel, with a p-value of less than 0.01, compared to that of untreated cells ( Figure 1F). In two negative controls,l ight irradiation or FMN incubation alone didnta ffect the m 6 Al evel, thus indicating that the native intracellular FMN (6.94 AE 0.90 nm,T able S2) [14] is not capable of demethylating m 6 Aa nd additional free FMN is required.…”