Blue light induced free radicals from riboflavin on E. coli DNA damage

Liang, Ji-Yuan; Yuann, Jeu-Ming P.; Cheng, Chien-Wei; Jian, Hong-Lin; Lin, Chin-Chang; Chen, Liang‐Yu

doi:10.1016/j.jphotobiol.2012.12.007

Cited by 71 publications

(47 citation statements)

References 13 publications

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“…It is noteworthy that except for the functional category of chaperone/heat shock proteins, the expression of genes belonging to these functional categories is up‐regulated. This may reflect the protective response against the particularly oxidative stress known to be induced by blue light (Liang et al, ). After a blue light treatment, up‐regulation of bacterial genes coding for chaperones/heat shock proteins would be an expected consequence of the high energy status associated to blue light.…”

Section: Resultsmentioning

confidence: 99%

Pseudomonas syringaepv. tomato exploits light signals to optimize virulence and colonization of leaves

Santamaría‐Hernando

Rodríguez‐Herva

Martínez-García

et al. 2018

Environmental Microbiology

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Light is pervasive in the leaf environment, creating opportunities for both plants and pathogens to cue into light as a signal to regulate plant-microbe interactions. Light enhances plant defences and regulates opening of stomata, an entry point for foliar bacterial pathogens such as Pseudomonas syringae pv. tomato DC3000 (PsPto). The effect of light perception on gene expression and virulence was investigated in PsPto. Light induced genetic reprogramming in PsPto that entailed significant changes in stress tolerance and virulence. Blue light-mediated up-regulation of type three secretion system genes and red light-mediated down-regulation of coronatine biosynthesis genes. Cells exposed to white light, blue light or darkness before inoculation were more virulent when inoculated at dawn than dusk probably due to an enhanced entry through open stomata. Exposure to red light repressed coronatine biosynthesis genes which could lead to a reduced stomatal re-opening and PsPto entry. Photoreceptor were required for the greater virulence of light-treated and dark-treated PsPto inoculated at dawn as compared to dusk, indicating that these proteins sense the absence of light and contribute to priming of virulence in the dark. These results support a model in which PsPto exploits light changes to maximize survival, entry and virulence on plants.

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Section: Resultsmentioning

confidence: 99%

Pseudomonas syringaepv. tomato exploits light signals to optimize virulence and colonization of leaves

Santamaría‐Hernando

Rodríguez‐Herva

Martínez-García

et al. 2018

Environmental Microbiology

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“…The conventional model describing the BLI susceptibility mechanism is through damage from the generation of ROS, most notably singlet oxygen (Farr & Kogoma, 1991;Feuerstein et al, 2005Feuerstein et al, , 2006Liang et al, 2013;Lipovsky et al, 2009Lipovsky et al, , 2010Lubart et al, 2011;Malik et al, 1990;Marugán et al, 2010). Studies also indicate that low-level stimulation of ROS can enhance proliferation of bacterial growth with the resulting daughter cells sometimes exhibiting altered replication rates (Dai et al, 2012;Lipovsky et al, 2009Lipovsky et al, , 2010Lubart, Lavi, Friedmann, & Rochkind, 2006;Lubart et al, 2011;Nussbaum et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Much of the previous work evaluating the efficacy of BLI in growth reduction of E. coli and other gram-negative bacteria investigated only one strain from each bacterial species (Alves et al, 2015;Hanakova et al, 2014;Liang et al, 2013;Nussbaum et al, 2002;Popov et al, 2005;Tschowri, Busse, & Hengge, 2009;Tschowri, Lindenberg, & Hengge, 2012). While analyzing a single, model strain provides an initial basis for comparison, understanding how different strains within a species respond to BLI is essential to develop successful therapeutic approaches against them, especially given the strain heterogeneity within species like E. coli (Croxen & Finlay, 2010).…”

Section: Strain-and Growth Phase-specific Responses To Bli 455 For mentioning

confidence: 99%

“…Previous studies indicated that light promotes PSs to an excited state, after which electrons (or energy) are transferred to molecules such as molecular oxygen, forming reactive oxygen species (ROS) ( Fig. S1b and (Feuerstein, Ginsburg, Dayan, Veler, & Weiss, 2005;Liang et al, 2013;Lubart et al, 2011;Malik, Hanania, & Nitzan, 1990;Marugán, van Grieken, Pablos, & Sordo, 2010)). Increased ROS levels can cause damage to cellular proteins, lipids, and DNA (Farr & Kogoma, 1991;Feuerstein, Moreinos, & Steinberg, 2006;Liang et al, 2013;Lipovsky, Nitzan, Friedmann, & Lubart, 2009;Lipovsky et al, 2010).…”

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confidence: 99%

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Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli

et al. 2017

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Blue light irradiation (BLI) is an FDA‐approved method for treating certain types of infections, like acne, and is becoming increasingly attractive as an antimicrobial strategy as the prevalence of antibiotic‐resistant “superbugs” rises. However, no study has delineated the effectiveness of BLI throughout different bacterial growth phases, especially in more BLI‐tolerant organisms such as Escherichia coli. While the vast majority of E. coli strains are nonpathogenic, several E. coli pathotypes exist that cause infection within and outside the gastrointestinal tract. Here, we compared the response of E. coli strains from five phylogenetic groups to BLI with a 455 nm wavelength (BLI 455), using colony‐forming unit and ATP measurement assays. Our results revealed that BLI 455 is not bactericidal, but can retard E. coli growth in a manner that is dependent on culture age and strain background. This observation is critical, given that bacteria on and within mammalian hosts are found in different phases of growth.

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“…The generation of ROS was expected during the isomerization of trans-CA, which is governed by the activated excitation state. Our aim was to improve the bactericidal activity of UV-A light mediated by ROS such as hydrogen peroxide and hydroxyl radicals Hamamoto et al, 2007 via the utilization of photo-activated compounds, in a similar way to photobactericidal agents such as riboflavin Liang et al, 2013, methylene blue Giroldo et al, 2009and rose Bengal Shrestha and Kishen, 2012 . If excess ROS are produced by the combination of trans-CA and UV-A, viable cells can suffer extensive damage.…”

Section: Synthesismentioning

confidence: 99%

Improved Photobactericidal Activity of Ultraviolet-A Light in Combination with Isomerizable <i>p</i>-Coumaric Acid Derivatives

et al. 2015

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In order to improve the photobactericidal activity of ultraviolet-A (UV-A) mediated by reactive oxygen species (ROS), the present study focused on trans-coumaric acid (trans-CA), which is isomerized by UV-A. Generation of ROS was expected during the isomerization of trans-CA. Trans-CA derivatives, in which the carboxyl group was modified with a methyl, n-butyl or phenyl group, thereby changing the interaction with the cellular membrane by quenching the anionic properties of the carboxyl group and changing the UV adsorption properties, were used. The photobactericidal activities of trans-CA derivatives were evaluated by using UV-A light (wavelength 350 to 385 nm). The number of surviving Escherichia coli NBRC12713 was determined by colony-forming assay. Derivative 4c, which was esterified with a phenyl group, reduced survival by more than 5.0-log at a dose of 7.4 J/cm(2) and by 3.2-log at a dose of 4.9 J/cm(2). This synergistic activity may have been caused by the absorption of photon energy from UV-A, which is attributable to the UV spectrum of 4c. The photobactericidal activity was comparable to that of riboflavin, a known photo-activated agent. Isomerized molecules serve as a promising lead for improving the photobactericidal activity of UV-A by activating molecule-mediated ROS generation.

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Blue light induced free radicals from riboflavin on E. coli DNA damage

Cited by 71 publications

References 13 publications

Pseudomonas syringaepv. tomato exploits light signals to optimize virulence and colonization of leaves

Pseudomonas syringaepv. tomato exploits light signals to optimize virulence and colonization of leaves

Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli

Improved Photobactericidal Activity of Ultraviolet-A Light in Combination with Isomerizable <i>p</i>-Coumaric Acid Derivatives

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