Blue light- and ubiquitin-dependent influence on phototropin 1 abundance and movement at the plasma membrane

Askinosie, Scott K.

doi:10.32469/10355/59818

Cited by 3 publications

(5 citation statements)

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“…Common career advice is to "find your calling," with a host of book titles boasting phrases such as Find Your Calling, Love Your Life; Find Your Calling and Feed Your Soul; and Do What You Love, The Money Will Follow (Askinosie & Askinosie, 2017;Finney & Dasch, 1998;Sinetar, 1989). When people feel a strong calling toward their work, they experience it with a sense of meaningfulness, passion, and deep fulfillment (e.g., Bunderson & Thompson, 2009;Wrzesniewski et al, 1997).…”

mentioning

confidence: 99%

Do what you love and you’ll never work a day in your life? Testing fundamental assumptions about calling, effort, and enjoyment

Tosti-Kharas,

Dobrow,

Kappes

2024

Journal of Management Scientific Reports

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We test the fundamental assumptions that people experiencing a stronger sense of calling invest more effort in their work tasks, and find those tasks more enjoyable, than people with a weaker sense of calling. Both assumptions have been expressed theoretically, yet received limited empirical support. Among 2,839 workers in a crowdsourced marketplace, we found that people with a stronger calling toward their work completed more of a relatively unengaging work task and enjoyed the task more than those with a weaker calling. The calling-effort relationship was particularly strong when there was no financial incentive for effort (i.e., paid a fixed amount), highlighting the risk of exploitation for strong-calling employees. People with stronger callings nonetheless responded to financial incentives—they completed more work when offered additional pay to do so. The relationship between calling and enjoyment of the task was particularly strong when there was a financial incentive for effort (i.e., paid piece-rate), indicating that extrinsic rewards did not “crowd out” intrinsic rewards. Our findings are thus consistent with research about the presence of multiple motives for behavior. Our empirical support for these assumptions using more appropriate, rigorous methods paves the way to further develop novel calling theory.

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confidence: 99%

Do what you love and you’ll never work a day in your life? Testing fundamental assumptions about calling, effort, and enjoyment

Tosti-Kharas,

Dobrow,

Kappes

2024

Journal of Management Scientific Reports

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“…In contrast, under low intensity blue light phot1 is monoubiquitinated rather than polyubiquitinated (Roberts et al, 2011). The function of phot1 monoubiquitination has recently been suggested as means to retain phot1 signaling at the plasma membrane (Askinosie, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Recent data suggests that the purpose of mono/multiubiquitination is to keep phot1 at the plasma membrane as a signaling competent receptor (Askinosie, 2016).…”

Section: A Role For Nph3 As a Substrate Adaptor In An E3 Ubiquitin Ligase Complexmentioning

confidence: 99%

“…In contrast, under low light, phot1 is solely monoubiquitinated (Roberts et al, 2011). A resent study has shown that this monoubiquitination promotes retention of dispersed phot1 at the plasma membrane as a means to facilitate signaling (Askinosie, 2016). Roberts (2013) mapped ubiquitination sites within phot1 and identified one site which was shown to be blue light-and NPH3-dependent: K899.…”

Section: Chapter 4 Conclusion and Future Directions Introductionmentioning

confidence: 99%

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Mechanisms of the phototropic pathway

Morrow¹

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Plants are sessile organisms and therefore are limited in their ability to gather resources. Therefore, they have evolved several mechanisms that aid them in their quest to gather light, water and nutrients. One such mechanism is phototropism, a plants ability to bend towards or away from a light source. This mechanism is mediated by the blue light photoreceptor phototropin (phot). Arabidopsis thaliana contains two phototropins, phot1 which is the primary photoreceptor under low intensity light and phot2, which acts redundantly with phot1 under high intensity light. The perception of blue light by the phototropins (phot1 and phot2) initiates signaling events that lead to a lateral redistribution of the plant hormone auxin; which ultimately results in differential growth and the bending response. In addition to phototropism, these proteins mediate several other growth and developmental responses such as leaf movement, chloroplast movement, and stomatal opening. A second protein which is critical to the phototropic response and interacts with phot1 is NONPHOTOTROPIC HYPOCOTYL3 (NPH3). In addition to phototropism, NPH3 has a role in phot1-mediated leaf movement. NPH3 has been shown to act as a substrate adapter in an E3 ubiquitin ligase complex with the protein CULLIN3 (CUL3). This CRL3NPH3 complex is responsible for ubiquitinating the phot1 photoreceptor in a blue light fashion. This ubiquitination has been shown to be necessary for the bending response, but it's role in receptor ubiquitination is still not fully understood. To better understand this component of phot1-mediated phototropism, we characterized an allelic series of NPH3 mutants to further understand the role of this substrate adapter in this mechanism. Additionally, we characterized several mutant plant lines containing a mutant phot1 protein in which critical ubiquitination sites were mutated in an effort to render the protein unable to be ubiquitinated to further understand phot1 ubiquitination.

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“…Recent data suggests that the purpose of mono/multiubiquitination is to keep phot1 at the plasma membrane as a signaling competent receptor (Askinosie & Liscum, 2016).…”

Section: Signal Transductionmentioning

confidence: 99%

Mechanisms of photoreceptor mediated responses in photomorphogenesis and phototropism

Willenburg¹

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Photoreceptor facilitated light perception provides plants with critical environmental information from which they can direct their growth. We investigated the early photomorphogenic responses in Glycine max and Glycine soja with the aim of elucidating the effect that domestication has had on early light responses. Our study indicates that G. max exhibits growth in darkness that is more characteristic of growth in light. This finding suggests that domestication has resulted in a crop species that is partially constitutively photomorphogenic. We additionally investigated the effects of blue light-induced ubiquitination sites that were ubiquitinated dependently by NON-PHOTOTROPIC HYPOCOTYL3 (NPH3) in the red light photoreceptor phytochrome A (phyA) in Arabidopsis thaliana. We discovered that transgenic lines expressing phyA with the ubiquitination sites K555 and K603 converted to non-ubiquitinatable arginine residues displayed reduced degradation in blue light and additionally reduced phototropic curvature in high intensity blue light, while functioning normally in other responses tested: very low and low intensity blue light phototropism, far-red light hypocotyl growth inhibition, and red light-induced phyA degradation. This finding suggests a mechanism for a more direct route of blue and red light-signaling crosstalk than has been previously shown.

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Blue light- and ubiquitin-dependent influence on phototropin 1 abundance and movement at the plasma membrane

Cited by 3 publications

References 0 publications

Do what you love and you’ll never work a day in your life? Testing fundamental assumptions about calling, effort, and enjoyment

Do what you love and you’ll never work a day in your life? Testing fundamental assumptions about calling, effort, and enjoyment

Mechanisms of the phototropic pathway

Mechanisms of photoreceptor mediated responses in photomorphogenesis and phototropism

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