Blue Light-Activated Riboflavin Phosphate Promotes Collagen Crosslinking to Modify the Properties of Connective Tissues

Kang, Yeyoung; Kim, Jae Hak; Kim, Seo Young; Koh, Won‐Gun; Lee, Hyun Jong

doi:10.3390/ma14195788

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…UV irradiation with a weak intensity of 3 mW cm −2 is applied for a period of approximately 30 min. Kang et al demonstrated that RFP-induced crosslinking increased the Young’s modulus and tensile strength of skin tissues by approximately three times by applying the same procedure to skin collagen as that for corneal collagen [ 46 ]. Blue light is safer than UV light when applied to the skin; therefore, visible light with a wavelength of 450 nm was employed.…”

Section: Riboflavin-induced Photochemistrymentioning

confidence: 99%

“…Similar to the cornea, skin tissue is exposed to the environment, and collagen is a significant component of the dermis. Applying RFP-mediated photochemistry to skin tissue increases its elasticity and strength [ 46 ]. For practical applications, further studies are required to achieve the delivery of sufficient amounts of RFP to the dermal layer for photochemistry.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

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Green Chemistry for Crosslinking Biopolymers: Recent Advances in Riboflavin-Mediated Photochemistry

et al. 2023

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Riboflavin (RF), which is also known as vitamin B2, is a water-soluble vitamin. RF is a nontoxic and biocompatible natural substance. It absorbs light (at wavelengths of 380 and 450 nm) in the presence of oxygen to form reactive singlet oxygen (1O2). The generated singlet oxygen acts as a photoinitiator to induce the oxidation of biomolecules, such as amino acids, proteins, and nucleotides, or to initiate chemical reactions, such as the thiol-ene reaction and crosslinking of tyramine and furfuryl groups. In this review, we focus on the chemical mechanism and utilization of the photochemistry of RF, such as protein crosslinking and hydrogel formation. Currently, the crosslinking method using RF as a photoinitiator is actively employed in ophthalmic clinics. However, a significant broadening is expected in its range of applications, such as in tissue engineering and drug delivery.

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Section: Riboflavin-induced Photochemistrymentioning

confidence: 99%

Section: Biomedical Applicationsmentioning

confidence: 99%

Green Chemistry for Crosslinking Biopolymers: Recent Advances in Riboflavin-Mediated Photochemistry

et al. 2023

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“…Collagen glycation also proved to be an effective stiffening method, although it is time consuming [ 81 , 82 ]. Finally, the use of ultraviolet crosslinking in the presence of riboflavin possesses the advantage of being a simple and effective method that may also act on a local scale: by using light masks that spatially filter light transmission, it is possible to create patterns or gradients of crosslinked fibrils in the gel [ 83 , 84 ]. Light irradiation, especially in the UV range, may induce cytotoxic effects.…”

Section: 3d Fibrillar Collagen Gelsmentioning

confidence: 99%

Collagen-Based Biomimetic Systems to Study the Biophysical Tumour Microenvironment

Cambi

Ventre

2022

Cancers

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The extracellular matrix (ECM) is a pericellular network of proteins and other molecules that provides mechanical support to organs and tissues. ECM biophysical properties such as topography, elasticity and porosity strongly influence cell proliferation, differentiation and migration. The cell’s perception of the biophysical microenvironment (mechanosensing) leads to altered gene expression or contractility status (mechanotransduction). Mechanosensing and mechanotransduction have profound implications in both tissue homeostasis and cancer. Many solid tumours are surrounded by a dense and aberrant ECM that disturbs normal cell functions and makes certain areas of the tumour inaccessible to therapeutic drugs. Understanding the cell-ECM interplay may therefore lead to novel and more effective therapies. Controllable and reproducible cell culturing systems mimicking the ECM enable detailed investigation of mechanosensing and mechanotransduction pathways. Here, we discuss ECM biomimetic systems. Mainly focusing on collagen, we compare and contrast structural and molecular complexity as well as biophysical properties of simple 2D substrates, 3D fibrillar collagen gels, cell-derived matrices and complex decellularized organs. Finally, we emphasize how the integration of advanced methodologies and computational methods with collagen-based biomimetics will improve the design of novel therapies aimed at targeting the biophysical and mechanical features of the tumour ECM to increase therapy efficacy.

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“…Visible light-mediated photo-cross-linking initiated the reaction by irradiating blue light to convert riboflavin phosphate (RFP), a vitamin B derivative, into an excited state. RFP is used as a photoinitiator because of its capacity to absorb light. − After being exposed to light, RFP gets excited and takes on electrons from electron donors, thus, transferring their energy. The intermediate receiving the energy oxidizes substrates, such as biomolecules, and this sequential reaction is called RF-mediated oxidation .…”

Section: Introductionmentioning

confidence: 99%

Visible Light-Mediated Environmentally Friendly and Universally Applicable Green Chemistry for Hair Cross-Linking

Kim

Choi³

et al. 2023

ACS Sustainable Chem. Eng.

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The cosmetics industry is experiencing a rise in the demand for eco-friendly products due to growing environmental awareness. In response, eco-friendly forms of raw materials, production processes, and packaging have been developed. For hair products, the research focus is on a harmless method of crosslinking hair using eco-friendly raw materials. In this regard, we have recently proposed clean and nontoxic one-step protein crosslinking using eco-friendly chemicals composed of riboflavin phosphate (RFP), citric acid (CA), and polyols. In this study, we further explored the optimal concentration that induced maximum hair cross-linking by adjusting the ratio of CA, isopentyldiol (IPD), and sorbitol required for hair keratin protein cross-linking. Various combinations of CA in the range of 0.5−3% and IPD and sorbitol in the range of 1−5% were used to induce RFP-mediated photo-cross-linking. As a result, the combination of CA 3%, IPD 5%, and sorbitol 3% induced optimal cross-linking and increased tensile stress and Young's modulus of hair by 41% and 44%, respectively. In addition, RFP treatment was applied to black, brown, and blond hair to confirm the variation in the photo-cross-linking reaction according to hair color. Photo-cross-linking was successfully induced in the hair of all colors, and the increase in mechanical properties and hair-setting effect were verified. Hair color had no discernible impact on visible light-mediated cross-linking. Lastly, we demonstrated that the visible light-mediated cross-linking method we developed can be used for any hair color, and it is expected to be widely adopted as an environmentally friendly hair care product.

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Blue Light-Activated Riboflavin Phosphate Promotes Collagen Crosslinking to Modify the Properties of Connective Tissues

Cited by 8 publications

References 26 publications

Green Chemistry for Crosslinking Biopolymers: Recent Advances in Riboflavin-Mediated Photochemistry

Green Chemistry for Crosslinking Biopolymers: Recent Advances in Riboflavin-Mediated Photochemistry

Collagen-Based Biomimetic Systems to Study the Biophysical Tumour Microenvironment

Visible Light-Mediated Environmentally Friendly and Universally Applicable Green Chemistry for Hair Cross-Linking

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