Blue LED light modulates inflammatory infiltrate and improves the healing of superficial wounds

Magni, Giada; Tatini, Francesca; Bacci, Stefano; Paroli, Gaia; Siena, Gaetano De; Cicchi, Riccardo; Pavone, Francesco S.; Pini, Roberto; Rossi, Francesca

doi:10.1111/phpp.12527

Cited by 23 publications

(24 citation statements)

References 10 publications

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“…Further studies in murine model enabled us to optimize treatment conditions to obtain a better healing process, testing lower fluences (21 J/cm 2 , 680 mW/cm 2 and 30 s treatment time) and thus reducing the photothermal effects. The results evidence a modulation of the inflammatory infiltrate and of the cytokines release [16]. The first studies in selected human patients [14,15] pointed out effects in the healing of hard-to-heal wounds that can be ascribed to a photobiomodulation process, with a negligible photothermal effect (irradiation parameters in patients: 120 mW/cm 2 , 60 s irradiation time and 7.2 J/cm 2 fluence).…”

Section: Discussionmentioning

confidence: 90%

“…We thus decided to investigate the interaction of this particular blue irradiation at a cellular level. In the present study, we focused on fibroblast derived from human healthy tissue, as we previously reported in vivo that the activity of fibroblasts is modulated by the blue LED light [16,17]. Moreover, they play an important role in the wound healing process and tissue morphology reconstruction.…”

Section: Introductionmentioning

confidence: 99%

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Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

et al. 2021

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In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here, we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts from healthy skin and keratinocytes. Different light doses (3.43, 6.87, 13.7, 20.6, 30.9 and 41.2 J/cm2) were used to treat the cells, evidencing inhibitory and stimulatory effects following a biphasic dose behavior. Electrophysiology was used to investigate the effects on membrane currents: healthy fibroblasts and keratinocytes showed no significant differences between treated and not treated cells. Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation: a significant decrease in peak intensity of healthy fibroblast was evidenced, while it is less pronounced in keratinocytes. In conclusion, we observed that the blue LED light can be used to modulate metabolism and proliferation of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

et al. 2021

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“…Blue light (wavelengths of 400-500 nm) has been widely used for curing the hyperbilirubinemia of infantile jaundice and the polymerization of dental composite (13)(14)(15). A study on the improvement of superficial wound healing after blue light irradiation in mice was also reported (16). Recently, we have published our experimental data showing that blue (450 nm) light irradiation without photosensitizers suppressed cancer cell proliferation, migration and invasion (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Photobiogoverning Role of Blue Light Irradiation on Viral Replication

Kang

Ryu

et al. 2021

Photochem & Photobiology

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Most recently, severe acute respiratory syndrome coronavirus-2 has triggered a global pandemic without successful therapeutics. The goal of the present study was to define the antiviral effect and therapeutic action of blue light irradiation in SARS-CoV-2-infected cells. Vero cells were infected with SARS-CoV-2 (NCCP43326) or mock inoculum at 50 pfu/well. After blue light irradiation, the inhibitory effect was assessed by qPCR and plaque reduction assay. When Vero cells were irradiated to blue light ranging from 1.6 to 10 J cm À2 , SARS-CoV-2 replication was inhibited by up to 80%. The antiviral effect of blue light irradiation was associated with translation suppression via the phosphorylation of eIF2a by prolonging endoplasmic reticulum (ER) stress. The levels of LC3A/B and Beclin-1, which are key markers of autophagy, and the levels of PERK and PDI for ER stress were highly increased, whereas caspase-3 cleavage was inhibited after blue light irradiation in the later stage of infection. Our data revealed that blue light irradiation exerted antiviral and photo-biogoverning activities by prolonging ER stress and stimulating autophagy progression during viral infection. The findings increase our understanding of how photo-energy acts on viral progression and have implications for use in therapeutic strategies against COVID-19.Abbreviations: SARS-CoV-2, severe acute respiratory syndrome coronavirus-2; 2-DE/MALDI-TOF, 2-dimensional gel electrophoresis/matrix-assisted laser desorption/ionizationtime of flight; ER, endoplasmic reticulum; LC3, light chain 3; PERK, protein kinase RNA-like endoplasmic reticulum kinase; PDI, protein disulfide isomerase; LASP-1, LIM and SH3 domain protein 1; TCTP, translationally controlled tumor protein; ENO-1, alpha-enolase; NAC, N-acetylcysteine; eIF2a, eukaryotic translation initiation factor 2 alpha.

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“…Further studies in murine model, enabled us to optimize treatment conditions to obtain a better healing process, testing lower fluences (21 J/cm 2 , 680 mW/cm 2 , 30 seconds treatment time) and thus reducing the photothermal effects. The results evidenced a modulation of the inflammatory infiltrate and of the cytokines release [14]. The first studies in selected human patients [12,13] pointed out effects in the healing of hard-to-heal wounds that can be ascribed to a photobiomodulation process, with a negligible photothermal effect (irradiation parameters in patients: 120 mW/cm 2 , 60 seconds irradiation time, 7.2 J/cm 2 fluence).…”

Section: Discussionmentioning

confidence: 99%

“…The main goal of the present study is to observe the effects at a cellular level of blue LED light emitting in the range 410-430 nm, in order to investigate if these effects can be ascribed to a PBM therapy with blue light. We focused in the experimental study on fibroblast derived from human healthy tissue, as we previously reported in vivo that the activity of fibroblasts is modulated by the blue LED light [14,15] and, moreover, they play an important role in the wound healing process and tissue morphology reconstruction. The simultaneous use of a well-known cellular model, HaCaT cells, and primary human cell cultures, gives us the opportunity to compare our result with the literature and at the same time to exploit the complexity of fresh, non-immortalized cell lines.…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Rossi

Magni

Tatini

et al. 2020

Preprint

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In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts and keratinocytes. Different light doses were used to treat the cells, evidencing inhibitory and stimulatory effects. Electrophysiology was used to investigate the effects on membrane currents, while Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation. In conclusion, we observed that the blue LED light can be used to modulate the activity of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

show abstract

Blue LED light modulates inflammatory infiltrate and improves the healing of superficial wounds

Cited by 23 publications

References 10 publications

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Evaluation of Photobiogoverning Role of Blue Light Irradiation on Viral Replication

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

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