Blue and red light affects morphogenesis and 20-hydroxyecdisone content of in vitro Pfaffia glomerata accessions

Silva, Tatiane Dulcineia; Batista, Diego Silva; Fortini, Evandro Alexandre; Castro, Kamila Motta de; Felipe, Sérgio Heitor Sousa; Fernandes, Amanda Mendes; Sousa, Raysa Mayara de Jesus; Chagas, Kristhiano; Silva, José Victor Siqueira da; Correia, Ludmila Nayara de Freitas; Farias, Letícia Monteiro; Leite, João Paulo Viana; Rocha, Diego Ismael; Otoni, Wagner Campos

doi:10.1016/j.jphotobiol.2019.111761

Cited by 38 publications

(19 citation statements)

References 66 publications

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“…The seedlings grown under B/R 3:1 and under monochromatic blue light showed a better photosynthetic performance index (Table 5) and higher photosynthetic rate (Figure 5), indicating that the blue wavelength increases the photosynthetic potential of B. gaudichaudii. Consistent with these data, seedlings of Pfaffia glomerata (Silva et al, 2020) and Fagus sylvatica (Košvancová-Zitová et al, 2009) also showed higher photosynthetic rates when grown in vitro under a higher ratio of blue light. The increase in the photosynthetic rate in response to blue light is probably due to the combined effect of this wavelength on different processes associated with carbon fixation, such as the induction of stomatal opening (Inoue and Kinoshita, 2017), the activation of genes encoding chloroplast proteins (Yu et al, 2010), the faster activation of Rubisco (Košvancová-Zitová et al, 2009), and even the movement of chloroplasts, to allow more efficient light capture (DeBlasio et al, 2003).…”

Section: Influence Of Light On the Physiological Characteristics Of B Gaudichaudiisupporting

confidence: 78%

“…One of the most effective ways to analyze the influence of light on the morphological, anatomical, and metabolic characteristics of plants is to use light-emitting diodes (LEDs; Silvestri et al, 2019 ; Chen et al, 2020 ). LEDs have numerous advantages over traditional lighting methods, such as low cost, small size, high energy efficiency, low heat emission, and strict control of the spectral composition, which allows the culture of plants at specific wavelengths (Monostori et al, 2018 ; Silva et al, 2020 ). The culture of plants using blue and red light plays an important role in the development and growth of the photosynthetic apparatus by regulating biomass production and allocation, plant growth, and antioxidant system activation.…”

Section: Introductionmentioning

confidence: 99%

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Combinations of Blue and Red LEDs Increase the Morphophysiological Performance and Furanocoumarin Production of Brosimum gaudichaudii Trécul in vitro

et al. 2021

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Brosimum gaudichaudii is a plant species with medicinal relevance due to its furanocoumarin accumulation. The accumulation of these compounds in the root promotes predatory extractivism, which threatens the conservation of the species. In addition, little is known about the conditions for culturing of this species in vitro. The present study aimed to investigate how the application of different spectra of LEDs (white, blue, red, and combinations of blue and red at 1:1 and 3:1 ratios) can impact the morphophysiological and biochemical characteristics of B. gaudichaudii under different in vitro conditions. To evaluate the production of furanocoumarins in its leaves, which are easy-to-collect perennial organs, we cultured nodal segments in 50-mL tubes with MS medium under 100 μmol m−2 s−1 light and a photoperiod of 16 h for 50 days. We then submitted the seedlings biometric, anatomical, biochemical, and physiological evaluations. The different spectral qualities influenced several characteristics of the seedlings. Plants grown under red light showed greater stem elongation and larger and thinner leaves, strategies aimed at capturing a higher ratio of radiant energy. Exposure to the blue/red ratio of 1:1 induced increases in the concentration of the furanocoumarin psoralen, probably due to the diversion of carbon from primary metabolism, which resulted in lower growth. Cultivation under blue light or blue:red light at 3:1 triggered anatomical and physiological changes that led to higher production of secondary metabolites in the leaves, and at the 3:1 ratio, the seedlings also had a high growth rate. These results highlight the fundamental role of light in stimulating the production of secondary metabolites, which has important implications for the production of compounds of interest and indirect consequences for the conservation of B. gaudichaudii.

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Section: Influence Of Light On the Physiological Characteristics Of B Gaudichaudiisupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Combinations of Blue and Red LEDs Increase the Morphophysiological Performance and Furanocoumarin Production of Brosimum gaudichaudii Trécul in vitro

et al. 2021

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“…Overall, the use of blue or red LED during in vitro propagation can be beneficial for the improvement of plant production. The application of blue and red light for the in vitro growth of medicinal plants has been reported in Ajuga bracteosa (Ali et al, 2019), Pfaffia glomerata (Silva et al, 2020), Fritillaria cirrhosa (Chen et al, 2020), and Lippia rotundifolia (de Hsie et al, 2019).…”

Section: Influence Of Light Qualities On Growth and Morphologymentioning

confidence: 99%

“…Particularly, under an in vitro environment, light triggers different metabolic activities of the plant (Kozai et al, 1997). Several reports have shown an improvement of in vitro growth in diverse plants by various combinations of light spectra (Batista et al, 2018;Ali et al, 2019;Chen et al, 2020;Silva et al, 2020). In addition, light quality can be applied for the elicitation of vital phytochemicals with pharmaceutical importance.…”

Section: Introductionmentioning

confidence: 99%

“…Polyphenolic compounds present in plants are associated with diverse functions in growth, defense, protection from UV radiation, and reproduction (Cheynier et al, 2013;Sharma et al, 2019;Tanase et al, 2019). Moreover, the light quality-mediated stimulation of the antioxidant system resulting in the production of secondary metabolites has been shown in several plants (Chen et al, 2020;Silva et al, 2020). In green vegetables, the supplementation of blue light increased the biomass and enhanced the content of vitamin C (Li and Kubota, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement of in vitro Growth in Scrophularia kakudensis—A Potential Medicinal Plant

et al. 2021

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The current study has determined the effect of red and blue lights on the enhancement of growth, antioxidant property, phytochemical contents, and expression of proteins in Scrophularia kakudensis. In vitro-grown shoot tip explants of S. kakudensis were cultured on the plant growth regulator-free Murashige and Skoog (MS) medium and cultured under the conventional cool white fluorescent lamp (control), blue light-emitting diodes (LED) light, or red LED light. After 4 weeks, growth, stomatal ultrastructure, total phenols and flavonoids, activities of antioxidant enzymes, and protein expressions were determined. Interestingly, blue or red LED treatment increased the shoot length, shoot diameter, root length, and biomass on comparison with the control. In addition, the LED treatments enhanced the contents of phytochemicals in the extracts. The red LED treatment significantly elicited the accumulation of flavonoids in comparison with the control. In accordance with the secondary metabolites, the LED treatments modulated the activities of antioxidant enzymes. Moreover, the proteomic insights using two-dimensional gel electrophoresis system revealed the proteins involved in transcription and translation, carbohydrate mechanism, post-translational modification, and stress responses. Taken together, the incorporation of blue or red LED during in vitro propagation of S. kakudensis can be a beneficial way to increase the plant quality and medicinal values of S. kakudensis.

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Impact of Abiotic Stresses on Production of Secondary Metabolites in Medicinal and Aromatic Plants

Jampílek,

Kráľová

2023

Environmental Science and Engineering

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Blue and red light affects morphogenesis and 20-hydroxyecdisone content of in vitro Pfaffia glomerata accessions

Cited by 38 publications

References 66 publications

Combinations of Blue and Red LEDs Increase the Morphophysiological Performance and Furanocoumarin Production of Brosimum gaudichaudii Trécul in vitro

Combinations of Blue and Red LEDs Increase the Morphophysiological Performance and Furanocoumarin Production of Brosimum gaudichaudii Trécul in vitro

Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement of in vitro Growth in Scrophularia kakudensis—A Potential Medicinal Plant

Impact of Abiotic Stresses on Production of Secondary Metabolites in Medicinal and Aromatic Plants

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