Blubber proteome response to repeated ACTH administration in a wild marine mammal

Deyarmin, Jared; Hekman, Ryan; Champagne, Cory D.; McCormley, Molly; Stephan, Alicia; Crocker, Daniel E.; Houser, Dorian S.; Khudyakov, Jane

doi:10.1016/j.cbd.2019.100644

Cited by 7 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They reported that genes encoding metabolic enzymes and extracellular matrix components were significantly downregulated in blubber and skeletal muscle during fasting, suggesting significant metabolic and structural reprogramming of tissues in response to food deprivation (Martinez et al, 2018;Wright et al, 2020). We also previously showed that experimental elevation of cortisol, which normally increases during fasting, resulted in altered expression of genes and abundance of proteins associated with insulin resistance, lipid metabolism, lipid droplet homeostasis and adipogenesis, among other functions, in NES blubber and skeletal muscle (Deyarmin et al, 2020(Deyarmin et al, , 2019Khudyakov et al, 2017Khudyakov et al, , 2015. However, more work is necessary to understand how these metabolic and cellular adjustments are coordinated within and across multiple tissues to maintain metabolic homeostasis during energy-intensive fasting periods in NES.…”

Section: Introductionmentioning

confidence: 81%

“…Peptide samples were diluted to 200 ng µl −1 with 0.1% formic acid in LC/MS-grade water and 5 μl were used per injection (with three injections per sample) for HPLC as described previously (Deyarmin et al, 2020). Mass spectrometry analyses were performed using an Orbitrap Fusion™ Tribrid™ mass spectrometer equipped with an EASY-Spray™ ion source (Thermo Fisher Scientific) operated in a data-dependent acquisition (DDA) manner by Xcalibur 4.0 software (Thermo Fisher Scientific).…”

Section: Lc-ms/msmentioning

confidence: 99%

See 1 more Smart Citation

Changes in apolipoprotein abundance dominate proteome responses to prolonged fasting in elephant seals

Khudyakov

Holser

Vierra

et al. 2022

Journal of Experimental Biology

View full text Add to dashboard Cite

Unlike many animals that reduce activity during fasting, northern elephant seals (NES) undergo prolonged fasting during energy-intensive life-history stages such as reproduction and molting, fueling fasting energy needs by mobilizing fat stores accrued during foraging. NES display several unique metabolic features such as high fasting metabolic rates, elevated blood lipid and high-density lipoprotein (HDL) cholesterol levels, efficient protein sparing and resistance to oxidative stress during fasting. However, the cellular mechanisms that regulate these adaptations are still not fully understood. To examine how metabolic coordination is achieved during prolonged fasting, we profiled changes in blubber, skeletal muscle and plasma proteomes of adult female NES over a 5 week fast associated with molting. We found that while blubber and muscle proteomes were remarkably stable over fasting, over 50 proteins changed in abundance in plasma, including those associated with lipid storage, mobilization, oxidation and transport. Apolipoproteins dominated the blubber, plasma and muscle proteome responses to fasting. APOA4, APOE and APOC3, which are associated with lipogenesis and triglyceride accumulation, decreased, while APOA1, APOA2 and APOM, which are associated with lipid mobilization and HDL function, increased over fasting. Our findings suggest that changes in apolipoprotein composition may underlie the maintenance of high HDL levels and, together with adipokines and hepatokines that facilitate lipid catabolism, may mediate the metabolic transitions between feeding and fasting in NES. Many of these proteins have not been previously studied in this species and provide intriguing hypotheses about metabolic regulation during prolonged fasting in mammals.

show abstract

Section: Introductionmentioning

confidence: 81%

Section: Lc-ms/msmentioning

confidence: 99%

Changes in apolipoprotein abundance dominate proteome responses to prolonged fasting in elephant seals

Khudyakov

Holser

Vierra

et al. 2022

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Lipid composition of blubber consists mainly of triacylglycerols and reflect both diet and de novo lipogenesis (Bottino, 1978;Gross, 2020;Hansen & Cheah, 1969;Iverson & Koopman, 2018;Kawai et al, 1988;Krahn et al, 2001Krahn et al, , 2004Lafontan & Langin, 2009;Linder et al, 2010;Lockyer et al, 1984;Miller et al, 2020;Ruchonnet et al, 2006;Tilbury et al, 1997;Waugh et al, 2012). Blubber is also an endocrine and complex organ involved in a multitude of physiological and metabolic processes including immune response, energy balance, and lipid metabolism (Deyarmin et al, 2019(Deyarmin et al, , 2020Galligan et al, 2018Galligan et al, , 2020Kershaw et al, 2018). Finally, blubber also serves as an efficient and adjustable thermal insulator, buoyancy adjuster, and hydrodynamic facilitator (Bagge et al, 2012;Iverson & Koopman, 2018;Lockyer, 1991).…”

Section: Introductionmentioning

confidence: 99%

A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway

Bories

Rikardsen

Leonards

et al. 2021

Ecology and Evolution

View full text Add to dashboard Cite

In cetaceans, blubber is the primary and largest lipid body reservoir. Our current understanding about lipid stores and uses in cetaceans is still limited, and most studies only focused on a single narrow snapshot of the lipidome. We documented an extended lipidomic fingerprint in two cetacean species present in northern Norway during wintertime. We were able to detect 817 molecular lipid species in blubber of killer whales ( Orcinus orca ) and humpback whales ( Megaptera novaeangliae ). The profiles were largely dominated by triradylglycerols in both species and, to a lesser extent, by other constituents including glycerophosphocholines, phosphosphingolipids, glycerophosphoethanolamines, and diradylglycerols. Through a unique combination of traditional statistical approaches, together with a novel bioinformatic tool (LION/web), we showed contrasting fingerprint composition between species. The higher content of triradylglycerols in humpback whales is necessary to fuel their upcoming half a year fasting and energy‐demanding migration between feeding and breeding grounds. In adipocytes, we assume that the intense feeding rate of humpback whales prior to migration translates into an important accumulation of triacylglycerol content in lipid droplets. Upstream, the endoplasmic reticulum is operating at full capacity to supply acute lipid storage, consistent with the reported enrichment of glycerophosphocholines in humpback whales, major components of the endoplasmic reticulum. There was also an enrichment of membrane components, which translates into higher sphingolipid content in the lipidome of killer whales, potentially as a structural adaptation for their higher hydrodynamic performance. Finally, the presence of both lipid‐enriched and lipid‐depleted individuals within the killer whale population in Norway suggests dietary specialization, consistent with significant differences in δ 15 N and δ 13 C isotopic ratios in skin between the two groups, with higher values and a wider niche for the lipid‐enriched individuals. Results suggest the lipid‐depleted killer whales were herring specialists, while the lipid‐enriched individuals might feed on both herrings and seals.

show abstract

Lipid-loss in blubber biopsies is universal in cetaceans highlighting a need for new health assessment measures

Ryan¹,

Kershaw

2022

Mar Biol

View full text Add to dashboard Cite

Blubber proteome response to repeated ACTH administration in a wild marine mammal

Cited by 7 publications

References 54 publications

Changes in apolipoprotein abundance dominate proteome responses to prolonged fasting in elephant seals

Changes in apolipoprotein abundance dominate proteome responses to prolonged fasting in elephant seals

A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway

Lipid-loss in blubber biopsies is universal in cetaceans highlighting a need for new health assessment measures

Contact Info

Product

Resources

About