Bloodstream Infections with Metallo-β-Lactamase-Producing
 Pseudomonas aeruginosa
 : Epidemiology, Microbiology, and Clinical Outcomes

Marra, Alexandre R.; Pereira, Carlos Alberto Pires; Gales, Ana Cristina; Menezes, Liana Carballo; Cal, Ruy Guilherme Rodrigues; Souza, José Marconi A. de; Edmond, Michael B.; Faro, Cynthia; Wey, Sérgio Barsanti

doi:10.1128/aac.50.1.388-390.2006

Cited by 82 publications

(72 citation statements)

References 18 publications

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“…The possible explanation for this event is the concomitant presence of another extended-spectrum beta-lactamase (Such as the Ambler's class A). Similar results were found in a work by Marra and coworkers (2006) [12], where, among four P. aeruginosa isolates positive for SPM-1, only two were resistant to ATM, while all strains were resistant to CPM and IPM. In this study, the authors also found isolates harboring the IMP-16, which were equally resistant to ATM.…”

Section: Resultssupporting

confidence: 78%

“…According to Marra et al (2006) [12] and Magalhães et al (2005) [13], the SPM-1 enzyme, which is highly prevalent in Brazil, could be equally detected by the 2-MPA method and the EDTA-based method. Zavascki et al (2005) [14] reported that 77.1% of their P. aeruginosa isolates, resistant to IPM, could be detected by 2-MPA and 87.5% of the strains carried the bla SPM-1 gene.…”

Section: Resultsmentioning

confidence: 99%

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Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

Wirth

Picoli²,

Cantarelli

et al. 2009

Braz J Infect Dis

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This study determined the prevalence of metallo-β β β β β-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Rio Grande do Sul, Brazil (twenty-three from a hospital in Porto Alegre City and eight isolates from a hospital in Vale dos Sinos Region). All strains suggestive of possessing MBLs by phenotypic methods were included in this study. Phenotypic detection of MBLs was carried out simultaneously by using both the MBL Etest® and disk approximation test using 2-mercaptopropionic acid close to a ceftazidime disk. Strains positive were further confirmed using molecular techniques for bla VIM , bla IMP and bla SPM-1 . The prevalence of MBLs from samples of inpatients from the hospital located in Porto Alegre was 30.4% and that of inpatients from Vale dos Sinos hospital was only 3.1%. Only MBL type SPM-1 was detected in these samples by molecular analysis and all were detected by the Etest® MBL strips. The prevalence of P. aeruginosa that produce MBLs can be markedly different in distinct geographical areas, even among different hospitals in the same area. In our study, the EDTA-based method was the only method able to detect all strains harboring the SPM-1 enzyme.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

Wirth

Picoli²,

Cantarelli

et al. 2009

Braz J Infect Dis

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“…Moreover, the treatment alternatives are unavailable, or expensive/toxic with poor outcome. [4] Plasmid mediated MBL genes spread rapidly to other species of gram negative bacilli; [5] therefore rapid detection of metallo-ß-lactamases production is necessary to modify therapy and to initiate effective infection control to prevent their dissemination. To date there has been one report from Pakistan suggesting presence of MBL producing isolate.…”

mentioning

confidence: 99%

Metallo-β-lactamase-producing clinical isolates of Acinetobacter species and Pseudomonas aeruginosa from intensive care unit patients of a tertiary care hospital

Irfan¹,

Zafar²,

Guhar³

et al. 2008

Indian J Med Microbiol

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Prompt detection of metallo-β-lactamase (MBL) producing isolates is necessary to prevent their dissemination. Frequency of MBLs producing strains among multidrug resistant (MDR) Acinetobacter species and Pseudomonas aeruginosa was evaluated in critical care patients using imipenem-EDTA disk method. One hundred MDR Acinetobacter spp. and 42 Pseudomonas aeruginosa were checked for MBL production, from January to June 2001. MBL was produced by 96.6 % of imipenem-resistant Acinetobacter isolates, whereas 100% imipenem-resistant Pseudomonas aeroginosa isolates were MBL producers. Carbapenem resistance in MDR Acinetobacter spp. and Pseudomonas aeruginosa isolates in this study was due to MBLs. This calls for strict infection control measures to prevent further dissemination. Key words: Acinetobacter spp, metallo-β-lactamase, Pseudomonas aeruginosaCarbapenem group of antibiotics play a vital role in the management of hospital-acquired gram negative infections, because of their broad spectrum activity and stability to hydrolysis by most of the β-lactamases, including extended spectrum β-lactamases (ESBLs). Nosocomial outbreaks of carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter spp. due to metallo-β-lactamases (MBLs) production have been reported from different regions. [1][2][3] The emergence of these MBLs in gram negative bacilli is becoming a therapeutic challenge as these enzymes possess high hydrolytic activity that leads to degradation of higher generation cephalosporins. Moreover, the treatment alternatives are unavailable, or expensive/toxic with poor outcome.[4] Plasmid mediated MBL genes spread rapidly to other species of gram negative bacilli; [5] therefore rapid detection of metallo-ß-lactamases production is necessary to modify therapy and to initiate effective infection control to prevent their dissemination. To date there has been one report from Pakistan suggesting presence of MBL producing isolate.[6] There is however need for a systematic study to assess the extent of this form of resistance amongst our isolates. The purpose of this study was to evaluate the metallo-ß-lactamase (MBL) production among multidrug resistant (MDR) Acinetobacter spp. and Pseudomonas aeruginosa, isolated from clinical specimens of intensive care unit patients. Materials and MethodsThis study was conducted in the clinical microbiology laboratory of The Aga Khan University Hospital, Karachi, Pakistan. One hundred MDR clinical isolates of Acinetobacter species and 42 Pseudomonas aeruginosa were studied for MBL production. These organisms were isolated from respiratory secretions, wound swabs, urine and blood culture specimens of intensive care unit patients, admitted from January to April 2001. A multidrug resistant isolate was deÞ ned as resistance to two or more drugs or drug classes of therapeutic relevance. [7] All the isolates of Pseudomonas aeruginosa were characterised to species level using standard procedures. Similarly, Acinetobacter isolates were characterised to the genus level using these proced...

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“…Other authors also had similar findings (Zavascki et al, 2006). In our study reveals that, P. aeruginosa infection was predominantly found among males 51% and another study showed 85.7% were most commonly seen in males infected with P. aeruginosa (Mara et al, 2006).…”

Section: Resultsmentioning

confidence: 75%

Performance of Modified Hodge Test for Detection of Carbapenemase Producing Clinical Isolates of Pseudomonas aeruginosa

Jayalakshmi¹,

Pandurangan²

2016

Int.J.Curr.Microbiol.App.Sci

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Bloodstream Infections with Metallo-β-Lactamase-Producing Pseudomonas aeruginosa : Epidemiology, Microbiology, and Clinical Outcomes

Cited by 82 publications

References 18 publications

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil

Metallo-β-lactamase-producing clinical isolates of <i> Acinetobacter</i> species and <i> Pseudomonas aeruginosa</i> from intensive care unit patients of a tertiary care hospital

Performance of Modified Hodge Test for Detection of Carbapenemase Producing Clinical Isolates of Pseudomonas aeruginosa

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