Blood vessel remodeling in pig ovarian follicles during the periovulatory period: an immunohistochemistry and SEM-corrosion casting study

Martelli, Alessandra; Palmerini, Maria Grazia; Russo, Valentina; Rinaldi, Carlo; Bernabò, Nicola; Giacinto, Oriana Di; Berardinelli, Paolo; Nottola, Stefania Annarita; Macchiarelli, Guido; Barboni, Barbara

doi:10.1186/1477-7827-7-72

Cited by 41 publications

(35 citation statements)

References 47 publications

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“…Morphometric and biochemical analyses were performed on at least 10 different serial sections/animals (3 sections for HE, 3 for vascular area, VA, and 4 for vascular endothelial growth factor, VEGF, expression analysis) [69]. Morphometric data were calculated on at least 8 different fields/sections, randomly distributed in the central (C) or peripheral (P) portion of the implanted area, were blind evaluated.…”

Section: Methodsmentioning

confidence: 99%

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

et al. 2013

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BackgroundEvidence has been provided that a cell-based therapy combined with the use of bioactive materials may significantly improve bone regeneration prior to dental implant, although the identification of an ideal source of progenitor/stem cells remains to be determined.AimIn the present research, the bone regenerative property of an emerging source of progenitor cells, the amniotic epithelial cells (AEC), loaded on a calcium-phosphate synthetic bone substitute, made by direct rapid prototyping (rPT) technique, was evaluated in an animal study.Material And MethodsTwo blocks of synthetic bone substitute (∼0.14 cm3), alone or engineered with 1×106 ovine AEC (oAEC), were grafted bilaterally into maxillary sinuses of six adult sheep, an animal model chosen for its high translational value in dentistry. The sheep were then randomly divided into two groups and sacrificed at 45 and 90 days post implantation (p.i.). Tissue regeneration was evaluated in the sinus explants by micro-computer tomography (micro-CT), morphological, morphometric and biochemical analyses.Results And ConclusionsThe obtained data suggest that scaffold integration and bone deposition are positively influenced by allotransplantated oAEC. Sinus explants derived from sheep grafted with oAEC engineered scaffolds displayed a reduced fibrotic reaction, a limited inflammatory response and an accelerated process of angiogenesis. In addition, the presence of oAEC significantly stimulated osteogenesis either by enhancing bone deposition or making more extent the foci of bone nucleation. Besides the modulatory role played by oAEC in the crucial events successfully guiding tissue regeneration (angiogenesis, vascular endothelial growth factor expression and inflammation), data provided herein show that oAEC were also able to directly participate in the process of bone deposition, as suggested by the presence of oAEC entrapped within the newly deposited osteoid matrix and by their ability to switch-on the expression of a specific bone-related protein (osteocalcin, OCN) when transplanted into host tissues.

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Section: Methodsmentioning

confidence: 99%

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

et al. 2013

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“…There were parallel increases in the expression of VEGFA mRNA in both granulosa and theca. Intriguingly, during early pre-antral follicle growth, there was a positive correlation between the degree of proliferation and vascular area (Martelli et al 2009). From this, it is tempting to speculate that preantral follicular selection is based on vascular supply.…”

Section: Pre-antral Follicular Vasculaturementioning

confidence: 95%

“…Not surprisingly, w40% of all proliferating cells in the theca are of endothelial origin (Martelli et al 2009). There were parallel increases in the expression of VEGFA mRNA in both granulosa and theca.…”

Section: Pre-antral Follicular Vasculaturementioning

confidence: 99%

Angiogenesis and vascular function in the ovary

Robinson

Woad²,

Hammond³

et al. 2009

Reproduction

232

210

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Ovarian function is dependent on the establishment and continual remodelling of a complex vascular system. This enables the follicle and/or corpus luteum (CL) to receive the required supply of nutrients, oxygen and hormonal support as well as facilitating the release of steroids. Moreover, the inhibition of angiogenesis results in the attenuation of follicular growth, disruption of ovulation and drastic effects on the development and function of the CL. It appears that the production and action of vascular endothelial growth factor A (VEGFA) is necessary at all these stages of development. However, the expression of fibroblast growth factor 2 (FGF2) in the cow is more dynamic than that of VEGFA with a dramatic upregulation during the follicular-luteal transition. This upregulation is then likely to initiate intense angiogenesis in the presence of high VEGFA levels. Recently, we have developed a novel ovarian physiological angiogenesis culture system in which highly organised and intricate endothelial cell networks are formed. This system will enable us to elucidate the complex inter-play between FGF2 and VEGFA as well as other angiogenic factors in the regulation of luteal angiogenesis. Furthermore, recent evidence indicates that pericytes might play an active role in driving angiogenesis and highlights the importance of pericyte-endothelial interactions in this process. Finally, the targeted promotion of angiogenesis may lead to the development of novel strategies to alleviate luteal inadequacy and infertility.

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“…Its development and maintenance depend on an adequate blood supply (reviewed in Fraser & Wulff (2003)). Prior to ovulation, the basement membrane of growing ovarian follicles constitutes the borderline between the vascularized and non-vascularized follicular compartments (Suzuki et al 1998, Grazul-Bilska et al 2007, Martelli et al 2009, Kowalewski et al 2015. Its rupture during ovulation is followed by rapid spreading of blood vessels into the follicular antrum and establishment of a dense vascular network that in mature CL provides virtually every lutein cell with access to a capillary.…”

Section: Introductionmentioning

confidence: 99%

Expression and functional implications of luteal endothelins in pregnant and non-pregnant dogs

Gram¹,

Latter²,

Boos³

et al. 2015

Reproduction

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Luteal development is regulated by many locally produced mediators, e.g., prostaglandins and angiogenic factors. However, the role and function of vasoactive factors in the canine corpus luteum (CL) remain largely unknown. Consequently, expression of the endothelin (ET) receptors-A and -B (ETA and ETB, revealing vasoconstriction and vasodilator properties respectively), the ET-converting enzyme (ECE1) and ET1, -2 and -3 were investigated in CL from non-pregnant dogs (days 5, 15, 25, 35, 45 and 65 post-ovulation), and at selected stages of pregnancy (pre-implantation, post-implantation, mid-gestation), and during normal and antigestagen-induced prepartum luteolysis/ abortion. The interrelationship between PGE2 and the ET system was investigated in PGE2-treated canine primary lutein cells from early CL. ET1 did not change significantly over time; ET2, ECE1 and ETB were elevated in early CL and were downregulated towards the mid/late-luteal phase. The prepartum increase of ET2 was significant. ET3 increased gradually, and was highest in late CL and/or at prepartum luteolysis. ETA remained constant until the late CL phase and increased only during prepartum luteolysis. ET1 was localized to the luteal cells, and ET2, ET3 and ETA to vascular endothelium. ECE1 and ETB were detected at both locations. Except for upregulated ET1 and lack of effect on ET2, antigestagen applied to mid-pregnant dogs evoked similar changes to those observed during normal luteolysis. PGE2 upregulated ETB in treated cells; ETA and ET1 remained unaffected, and ET2 decreased. A modulatory role of the ETs in canine CL, possibly in association with other factors (e.g., PGE2 and progesterone receptor), is strongly indicated.Reproduction (2015) 150 405-415

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Blood vessel remodeling in pig ovarian follicles during the periovulatory period: an immunohistochemistry and SEM-corrosion casting study

Cited by 41 publications

References 47 publications

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

Angiogenesis and vascular function in the ovary

Expression and functional implications of luteal endothelins in pregnant and non-pregnant dogs

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