Blood triggered rapid release porous nanocapsules

Gustafson, Tiffany P.; Dergunov, Sergey A.; Akers, Walter J.; Cao, Qian; Magalotti, Selena; Achilefu, Samuel; Pinkhassik, Eugene; Berezin, Mikhail Y.

doi:10.1039/c3ra22693j

Cited by 17 publications

(27 citation statements)

References 49 publications

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“…32 Our spectroscopy study showed that it is not the case. An addition of serum to a free HITC dye leads to a substantial bathochromic shift (10 nm) (Fig.…”

Section: Resultsmentioning

confidence: 78%

Temperature-dependent shape-responsive fluorescent nanospheres for image-guided drug delivery

Tourkakis

Berezin³

et al. 2016

J. Mater. Chem. C

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Temperature-responsive nanoparticles used in conjunction with hyperthermia promise to provide synergistic effects for increasing drug efficacy. We propose a near-infared (NIR) fluorescent system based on a upper critical solution temperature (UCST) polymer, ISP2, integrated with a NIR fluorescent dye HITC for in vivo tracking. The system forms a nanoparticle that increases its volume as temperature increases, similar to the expansion of a Hoberman sphere. The nanospheres nearly doubled in size, from 80 nm to 140 nm, during a temperature increase from 40°C to 60°C

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“…32 Our spectroscopy study showed that it is not the case. An addition of serum to a free HITC dye leads to a substantial bathochromic shift (10 nm) (Fig.…”

Section: Resultsmentioning

confidence: 78%

Temperature-dependent shape-responsive fluorescent nanospheres for image-guided drug delivery

Tourkakis

Berezin³

et al. 2016

J. Mater. Chem. C

Self Cite

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“…Similarly, encapsulation of fluorophores in micelles, liposomes, or polymeric nanoparticles affects their lifetime and, hence, their anisotropy. 53 Ionic strength does not directly affect the FA. However, very high affinity interactions (low-nM) are problematic for FA assays because they require the use of very low fluorophore concentrations, resulting in weak fluorescent signal.…”

Section: Steady-state Fluorescence Anisotropymentioning

confidence: 98%

“…Similarly, FA has validated the attachment of nanoparticles to fluorophore labeled DNA. 90 Gustafson et al 53 used FA to validate the encapsulation of an NIR imaging agent into porous nanocapsules designed for blood-triggered rapid release of cargo in emergency applications.…”

Section: Novel Applications: Biologics Nanotechnology and Imaginmentioning

confidence: 99%

Fluorescence anisotropy (polarization): from drug screening to precision medicine

Zhang

Berezin

2015

Expert Opinion on Drug Discovery

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Introduction Fluorescence anisotropy (FA) is one of the major established methods accepted by industry and regulatory agencies for understanding the mechanisms of drug action and selecting drug candidates utilizing a high-throughput format. Areas covered This review covers the basics of FA and complementary methods, such as fluorescence lifetime anisotropy and their roles in the drug discovery process. The authors highlight the factors affecting FA readouts, fluorophore selection, and instrumentation. Furthermore, the authors describe the recent development of a successful, commercially valuable FA assay for Long QT syndrome drug toxicity to illustrate the role that FA can play in the early stages of drug discovery. Expert opinion Despite the success in drug discovery, the FA-based technique experiences competitive pressure from other homogeneous assays. That being said, FA is an established yet rapidly developing technique, recognized by academic institutions, the pharmaceutical industry, and regulatory agencies across the globe. The technical problems encountered in working with small molecules in homogeneous assays are largely solved, and new challenges come from more complex biological molecules and nanoparticles. With that, FA will remain one of the major work-horse techniques leading to precision (personalized) medicine.

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“…Recently, we created NCs capable of burst release of encapsulated content upon contact with blood [84]. Rapid release is achieved by the expansion of intrinsic nanopores in the NC shell caused by the conformational change due to interactions with albumin.…”

Section: Biomedical Applications Of Polymer Nanocapsulesmentioning

confidence: 99%