Blood transfusion practices affect CD4+ CD25+ FOXP3+ regulatory T cells/T helper-17 cells and the clinical outcome of geriatric patients with hip fracture

Abstract

Hip fracture (HF) is common among older individuals and associated with high mortality, poor vitality and functional impairment. HF patients suffer whole body immunological changes and that lead to severe consequences, including immobilization, physical impairment and a high risk of complications. The objective of this study was to decipher the pattern of dynamic immunological changes, especially in two major T cell subsets, CD4 + CD25 + FOXP3 + regulatory T (Treg) cells and T helper-17 (Th17) cells, and their balance, during the hospital stay and to observe whether blood transfusion could influence these cells and clinical patietns’ prognosis. In this study, ninety-eight consecutive HF patients were initially enrolled, and finally fifty-one patients qualified for the study, and correlation analysis of their clinical parameters was carried out to predict the meaning of their distribution in clinical practice. Our results showed that the frequency of Tregs gradually decreased, while the frequency of Th17 cells slowly increased in HF patients who received blood transfusion. The Treg frequency was inversely correlated with the level of hemoglobin (Hb), and Th17 cell frequency was positively related to fluctuations in Hb levels in HF patients after trauma. HF patients with a better prognosis and survival time showed decreased a Treg frequency and a decreased Treg/Th17 ratio. Transfusion helped reverse the imbalance in the frequencies of Tregs and Th17 cells and the Treg/Th17 ratio and especially contributed to a better outcome in HF patients with moderate-to-severe anemia. In conclusion, a higher frequency of peripheral blood Tregs and a higher Treg/Th17 ratio may be associated with unfavorable outcomes in HF patients, and blood transfusion may benefit moderate-to-severe HF patients rebalance their immune response.

Comprehensive analysis of the potential cuproptosis-related biomarker LIAS that regulates prognosis and immunotherapy of pan-cancers

“…Cancer-associated fibroblast was reported to induce cancer cell proliferation, invasion, and metastasis through various pathways ( 28 , 29 ). Moreover, Tregs that expressed the transcription factor Foxp3 could possess importance in immune homeostasis ( 26 , 30 ). In this study, we found that LIAS expression was strongly correlated with the infiltration of immune cells, including B cell, cancer-associated fibroblast, T cell CD8+, DC, macrophage, and Tregs.…”

Thorough examination of the potential biological implications of the cuproptosis-related gene LIPT2 in the prognosis and immunotherapy in pan-cancer

“…These findings suggest that LIPT2 plays a [41]. Regulatory T cells (Tregs) contribute to immune homeostasis through diverse pathways [42]. In our investigation, we explored the relationship between LIPT2 expression levels and immune infiltration across GBM, LIHC, and PCPG tumors.…”

Does Autologous Transfusion Decrease Allogeneic Transfusion in Liposuction Surgery of Lymphedema Patients?

“…For instance, during allogeneic transfusion, Th2 cell is activated and the response of Th1 cell is thus decreased ( 23 ). Other mechanisms of immunomodulation might include the balance between T regulatory cell (Treg) and Th 17 cells: transfusion helped reverse the imbalance of Treg/Th17 ratios in hip fracture patients evidenced by the decrease and increased frequency of Tregs and Th17, respectively, after blood transfusion ( 24 ). In addition, in polytransfused sickle cell disease patients, high levels of CXCR5 + PD1 + CD4 + T lymphocyte (TL) may be a biomarker for the inhibited functions of T cells ( 25 ).…”