Blood-Tissue Barriers in the Male Reproductive Tract of the Dog: A Morphological Study Using Lanthanum Nitrate as an Electron-Opaque Tracer

Mann, Mireille Cambrosio; Frieß, A. E.; Stoffel, Michael

doi:10.1159/000072719

Cited by 18 publications

(6 citation statements)

References 34 publications

(46 reference statements)

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“…Male ZO-2 chimera show reduced fertility and pathological changes in the testis. Lanthanum tracer experiments showed a compromised BTB function in these mice 42112. Based on the gene expression and localization analyses, the authors found that the expression level of ZO-1 , ZO-3 , Cldn-11 and Ocln is not apparently affected when compared to the controls.…”

Section: Defects In Spermatogenesis In Ko Mice Of Btb-associated Protmentioning

confidence: 95%

“…Lanthanum tracer experiments showed a compromised BTB function in these mice. 42 112 Based on the gene expression and localization analyses, the authors found that the expression level of ZO-1 , ZO-3 , Cldn-11 and Ocln is not apparently affected when compared to the controls. ZO-1 and Ocln still localize to the BTB region, but Cldn-11 and Connexin43 are misslocalized from BTB.…”

Section: Defects In Spermatogenesis In Ko Mice Of Btb-associated Protmentioning

confidence: 99%

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Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice

et al. 2014

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The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferous tubes. It is a compound proteinous structure, composed of several types of cell junctions including tight junctions (TJs), adhesion junctions and gap junctions (GJs). Some of the junctional proteins function as structural proteins of BTB and some have regulatory roles. The deletion or functional silencing of genes encoding these proteins may disrupt the BTB, which may cause immunological or other damages to meiotic and postmeiotic cells and ultimately lead to spermatogenic arrest and infertility. In this review, we will summarize the findings on the BTB structure and function from genetically-modified mouse models and discuss the future perspectives.

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Section: Defects In Spermatogenesis In Ko Mice Of Btb-associated Protmentioning

confidence: 95%

Section: Defects In Spermatogenesis In Ko Mice Of Btb-associated Protmentioning

confidence: 99%

Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice

et al. 2014

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“…1995; Lopez et al. 1997; Cambrosio Mann et al. 2003), the molecular architecture of inter‐Sertoli and epididymal epithelial TJs is unrevealed in male reproductive tracts of rabbit.…”

Section: Introductionmentioning

confidence: 99%

Expression of Occludin in Testis and Epididymis of Wild Rabbits, Lepus sinensis coreanus

Yoon

Park

Nah

et al. 2009

Reprod Domestic Animals

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Tight junctions (TJs) in inter-Sertoli junctional areas and epididymal epithelia build up the blood-testis barrier (BTB) and the blood-epididymal barrier (BEB), respectively. In this study, the expression of occludin, an integral member of the TJs, was examined in testis and different regions of epididymis of Lepus sinensis coreanus, an Korean wild rabbit species. In testis, intense occludin immunoreactivity was found in the basally located inter-Sertoli junctional area together with diffused immunoreactivity of occludin in the cytoplasm of Sertoli cells. It can be suggested that occludin is one of the robust elements of BTB in seminiferous tubules of rabbit testis. In proximal and distal caput epididymis, occludin immunoreactivity was found in the lateral as well as apical contacts of epithelial cells. In corpus epididymis, intense occludin immunoreactivity was found in the basolateral as well as apical contacts of epithelial cells together with cytoplasmic signal. In cauda epididymis, occludin immunoreactivity in luminal epithelia was relatively strong but largely found in the cytoplasm. This suggests that intriguing regulatory mechanisms differentially recruit occludin to the TJ in the different regions of epididymal epithelia. The differences in the subcellular localization as well as expression levels of occludin among the epididymal segments may reflect differential paracellular permeability of epithelia along the epididymal tubules and be correlated with sperm maturation in rabbit. In Western blot, a major form of occludin was MW 62 kDa together with small fragments of MW 34-39 kDa in testis and epididymis, suggesting the peptide cleavage of occludin. This is the first report on the molecular nature of TJs in a wild rabbit testis and epididymis.

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“…Considering the inefficiency and the aggressive mode of cancer treatments, numerous efforts were made to find decent therapies with more specificity and safety among them during recent years. The most appropriate candidate in this area is the genes explicitly expressed in the testis tissue while not expressed or little expressed in other healthy tissues of the body [11,12]. Accordingly, testis cancer genes are promising targets for cancer vaccines and immunotherapy.…”

Section: Discussionmentioning

confidence: 99%

The Evaluation of RHOXF1 Gene Expression in Different Cell Lines

2019

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Evaluating RHOXF1 Gene Expression in Different Cell LinesBackground: RHOXF1 transcription factor is highly expressed in reproductive tissues and probably plays a role in spermatogenesis. RHOXF1 is expressed in some cancer-derived cell lines, too. The antiapoptotic role of RHOXF1, together with its hormone-dependent function, may increase its probable role in tumorigenesis as well as spermatogenesis. RHOXF1 represents the properties of cancer-testis genes-the promising targets for immunotherapy. Therefore, its study in cancer cell lines can considerably contribute to future research on therapeutic interventions. This study used both experimental and in silico investigation of RHOXF1 gene expression in different cancer cell lines. Materials and Methods:In the present study, we investigated RHOXF1 in silico gene expression levels in breast cancer and prostate cancer cell lines as well as in the Human Protein Atlas, GEMiCCL, and Expression Atlas databases. We also evaluated HEK 293 cells using RT-PCR. Finally, the results were compared and analyzed. Results:The obtained data showed an increase in RHOXF1 in cilico expression levels in the MCF7 cell line, which has a clear contradiction with the Human Protein Atlas and GEMiCCL databases. In this study, RHOXF1 gene expression was not observed in HEK293, MDA-MB-231, PC3, and DU145. The expression of the RHOXF1 gene in the database was also absent in some other cell lines, including HEK 293. Conclusion:We discovered the discrepancy of some databases with the experimental findings, also we found higher expression levels of the RHOXF1 gene as a cancer-testis gene in the MCF7 breast cancer cell line, which can be investigated as a possible candidate for immunotherapy.

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Blood-Tissue Barriers in the Male Reproductive Tract of the Dog: A Morphological Study Using Lanthanum Nitrate as an Electron-Opaque Tracer

Cited by 18 publications

References 34 publications

Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice

Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice

Expression of Occludin in Testis and Epididymis of Wild Rabbits, Lepus sinensis coreanus

The Evaluation of RHOXF1 Gene Expression in Different Cell Lines

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