Blood thyroglobulin and TSH receptor mRNA detection by RT-PCR in the follow-up of differentiated thyroid cancer patients

Stabilization of circulating thyroglobulin mRNA transcripts in patients treated for differentiated thyroid carcinoma

“…Collectively, this indicates that for blood stored in K 2 -EDTA tubes, the utility of extracted RNA for measurement of TG expression levels decreases following storage at room temperature for 72 h. Several of the studies involving development of TG assays based on semi-quantitative RT-PCR for measurement of TG mRNA levels in blood use K 2 -EDTA tubes for collection of blood. 14,16,17 Although blood samples were processed immediately in most of these studies, the study by Eszlinger et al and our findings suggest that precaution should be taken using K 2 -EDTA tubes for collection of blood when measuring TG mRNA levels. Eszlinger et al 12 did not use K 2 -EDTA tubes for collection of blood when quantifying TG mRNA since RNA yield and quality was relatively low.…”

“…5 New methods are under development to prevent interference by TGAb and promising methods consist of TG assays based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) [6][7][8] and TG assays based on real-time PCR (RT-PCR). [9][10][11][12][13][14][15][16][17][18] In contrast to other TG assays, the RT-PCR-based TG assays allow the detection of TG mRNA levels in blood. Despite promising, the outcome of studies involving TG assays based on semiquantitative RT-PCR has been variable.…”

“…Some find a correlation between TG mRNA levels and the presence of disease 9,11 and others do not. 10,[12][13][14][15][16][17] Additionally, Boldarine et al were able to differentiate DTC patients free of disease from those with metastases using quantitative RT-PCR for measurement of blood TG mRNA levels. 18 As previously noticed by several groups, this discrepancy in the outcome of RT-PCR-based TG assays could be related to methodological aspects such as different mRNA extraction protocols, different normalization strategies and the use of different primers.…”

“…18 As previously noticed by several groups, this discrepancy in the outcome of RT-PCR-based TG assays could be related to methodological aspects such as different mRNA extraction protocols, different normalization strategies and the use of different primers. [17][18][19][20] Moreover, preanalytical factors as type of collection tube, storage time and temperature, if not properly recognized and controlled, may also contribute to this discrepancy since they may influence on TG mRNA quality and stability and lead to induction or down-regulation of TG mRNA expression. Interestingly, a number of studies show that in blood, alteration of gene expression occurs following short ex vivo incubation as a result of induction, down-regulation or RNA degradation.…”

Somatostatin receptors as a new active targeting sites for nanoparticles

“…GPCRs can be known as an excellent target for many drugs. Recent studies have reported that many GPCRs, such as chemokine receptors (Singh et al, 2010, Nimmagadda, 2012), endothelin receptors (Jia et al, 2008, Liakou et al, 2012), TSH receptor (Antonelli et al, 2008, Torosian et al, 2010), LH receptors (Szepeshazi et al, 2007), lysophosphatidic acid receptors (Dorsam and Gutkind, 2007), SSTRs (Msaouel et al, 2009, Luboldt et al, 2010, Oddstig et al, 2011, Sun and Coy, 2011, Treglia et al, 2012), estrogen receptors (Deroo and Korach, 2006), are expressed in cancers (Dorsam and Gutkind, 2007).…”