Blood protein polymorphisms and the gut bacteria: impact of probiotic Lactobacillus acidophilus Narine on Salmonella carriage in sheep

Abstract

Related to previous reports on correlations between an animal’s genotype, its commensal microbiota, and the ability to resist infections, the aim of the current study was to investigate the associations between sheep genotype and 5-methylcytosine (5-mC) DNA methylation patterns, sheep genotype and cell surface hydrophobicity of sheep gut commensal bacteria. In addition, the effect of the probiotic Lactobacillus acidophilus strain INMIA 9602 Er 317/402 (probiotic formulation Narine) on Salmonella carriage in sheep at Armenian farms was also investigated. Allelotypes and genotypes of different pathogen-sensitive sheep breeds from Armenian farms were studied based on genetic markers of blood transferrin, albumin, and ceruloplasmin. Additionally, the differences between the breeds of Mazekh, Balbas, and Mazekh/Balbas hybrids were reported. The relationship between host sheep blood transferrin and albumin polymorphisms and cell surface hydrophobicity/5-mC DNA methylation patterns from the predominant gut commensal bacteria was shown. The Narine probiotic eliminates Salmonella from the sheep gut microbiota. At the same time, no significant changes in the percentage of 5-mC DNA methylation of predominant gut bacteria after probiotic administration were observed. The evaluation of bacterial cell surface hydrophobicity, the most significant factor affecting bacterial adhesion, as well as 5-mC DNA methylation, might be used for specific sheep husbandry/breeding programs. This study suggests that the commercial probiotic Narine could potentially be used to reduce Salmonella carriage in sheep.

Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli

“…317/402 from the commercial synbiotic “NARUM CAPS FAST” and commercial synbiotic “NARUM TAB”, (accessed on 28 December 2021)) were also used. * Predominated gut isolates from the sheep with the lowest cell surface hydrophobicity [ 59 ]. p 1 —comparison with the human/sheep/milk LAB probiotics (and Bacillus strains).…”

“… X Bacteria were grown in the growth medium: E. coli (LB medium); LAB and Bacillus (MRS broth). * Predominated gut isolates from the sheep with the lowest cell surface hydrophobicity [ 59 , 60 ]. ^ Probiotic bacterial strains of fish/shrimp origin from the microbial collections of the Southern Federal University of Russia ( Bacillus subtilis str.…”

One Health Probiotics as Biocontrol Agents: One Health Tomato Probiotics

“…The term “probiotics” as “active substances that are essential for a healthy development of life” was described by Werner Kollath in 1953 and by Ferdinand Vergin in 1954 [ 59 , 60 ]. Probiotics are alive bacterial cells that, when administered in adequate amounts, benefit the host’s health [ 61 ] and can have a substantial impact on the functionality of human and animal [ 48 , 62 , 63 , 64 , 65 , 66 , 67 ] organisms, as well as on plants [ 68 ]. Plant probiotics are used to increase resistance to pathogens [ 69 ] and improve yields by reducing or even eliminating chemical fertilizers [ 68 ].…”

Host genetics and gut microbiota composition: Baseline gut microbiota composition as a possible prognostic factor for the severity of COVID-19 in patients with familial Mediterranean fever disease

“…On the other hand, the associations between host physiology and gut bacteria (Khan et al, 2019 ; Ragonnaud and Biragyn, 2021 ; Tsaturyan et al, 2022b ), including host blood characteristics and gut bacteria (Pluznick, 2014 ; Balayan et al, 2015 ; Pepoyan et al, 2015a , 2020c ), gut microbiota composition, and COVID-19-accompanying diseases as well as between host gender and microbiota composition are at the researchers' attention (Pepoyan et al, 2021 ). Faecalibacterium prausnitzii, Eubacterium rectale , and Bifidobacteria, the gut commensals with known immunomodulatory potential, were described to be underrepresented in patients with COVID-19.…”