Blood plasma thermal behavior and protein oxidation as indicators of multiple sclerosis clinical status and plasma exchange therapy progression

Oxidized Milk Induces Spatial Learning and Memory Impairment by Altering Gut Microbiota in Offspring Mice during Pregnancy and Lactation

“…Similarly, our previous study showed that administration of freeze-dried milk powder containing high and low doses of dityrosine (2.8 and 1.4 mg/kg) increased oxidative stress and inflammation in rats and impaired learning and memory . Moreover, intake of different protein diets during lactation may affect infant growth and development . Previous studies reported the presence of casein oxidation products in spray-dried milk powder, and ingestion of milk powders containing casein oxidation products reduced individual growth responses and caused infant eczema and dry feces. , Fenaille et al.…”

“…The Organization for Economic Cooperation and Development (OECD) and the Food and Agriculture Organization of the United Nations (FAO) clearly stated in the ″Agricultural Outlook Report 2019-2028″ that the production and consumption of dairy products are expected to increase in the coming years. Raw milk and dairy products contain more unsaturated fatty acids, radiation, free radicals, and metal catalysts, they may induce milk to oxidize during processing and storage, causing changes in its physicochemical properties, such as gelling and emulsification, and changes in functions, such as hydration and digestion, affecting nutritional quality. − In addition, if dairy oxidatively modified products accumulate in the body, they may induce pathological changes such as aging, diabetes, and chronic kidney disease. − Over the past decades, researchers have reported various oxidants during food processing and storage, for instance, radiation, free radicals, and high oxygen, not only easily cause lipid oxidation rancidity but also induce protein oxidation. − Proteins, peptides, and amino acids undergo oxidative damage when subjected to ·OH and O 2– environments. , In addition, proteins and amino acids are strongly oxidized when placed in metal refinement oxidation systems, and proteins were mainly affected by ·OH. , Protein oxidation can produce a variety of chemical modifications resulting in oxidation products, including the formation of carbonyl derivatives, amino acid side-chain modifications, protein backbone cleavage, and formation of protein cross-linkages. , Some studies highlight that potentially toxic products can be ingested through food that is absorbed across the intestines and distributed in blood. , In the gastrointestinal tract and various internal organs, cellular responses to molecular changes in oxidative modification of proteins often involve specific signaling pathways . Our previous studies showed that oxidized casein damages the antioxidant defense system and causes oxidative stress and inflammation through the accumulation of protein oxidation products, such as dityrosine (Dityr), 3-nitrotyrosine (3-NT), and advanced oxidation protein products (AOPPs) .…”

“…18,19 Some studies highlight that potentially toxic products can be ingested through food that is absorbed across the intestines and distributed in blood. 20,21 In the gastrointestinal tract and various internal organs, cellular responses to molecular changes in oxidative modification of proteins often involve specific signaling pathways. 22 Our previous studies showed that oxidized casein damages the antioxidant defense system and causes oxidative stress and inflammation through the accumulation of protein oxidation products, such as dityrosine (Dityr), 3-nitrotyrosine (3-NT), and advanced oxidation protein products (AOPPs).…”

Comparison of human blood serum DSC profiles in aqueous and PBS buffer solutions

“…Recently, it has been recognized as having potential in diagnostics and monitoring of diseases. DSC has been used to characterize thermal denaturation transitions in various biological fluids, in particular, blood plasma [1][2][3][4][5][6][7][8][9], serum [10][11][12][13][14][15][16], synovial fluid [17][18][19], cerebrospinal fluid [20,21]. This highly sensitive method allows detecting, registering and analysis the changes taking place in various diseases, during treatment, after high-intensity exercise or because of other efforts and stressors .…”

“…Dilution of serum samples in PBS is the most common practice as it provides the closest to physiological conditions. Most publications showing the diagnostic power of DSC have analyzed plasma profiles in pH 7.4 buffer solutions [1][2][3][4][5][6][7][8][9]. We also used this buffer in some of our earlier research [14,15,24].…”