Blood Plasma Levels of Volatile Chlorinated Solvents and Metabolites in Occupationally Exposed Workers

Pfaffenberger, C.D.; Peoples, A.; Briggle, Thomas V.

doi:10.1016/s0166-1116(08)72136-8

Cited by 1 publication

(2 citation statements)

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“…As previously mentioned, pharmacokinetic modeling of TCE exhibits complicated conversion of in vivo to in vitro concentrations ( U.S. Environmental Protection Agency [U.S. EPA], 2011 ). We used a much lower TCE concentration in the present study compared to our previously published work ( Abdraboh et al, 2017 ), however, the used dose (0.25 μM which is equivalent to 32.85 μg/L) is still higher than the reported human serum concentrations ( Pfaffenberger et al, 1984 ; Jia et al, 2012 ).…”

Section: Resultsmentioning

confidence: 84%

“…It is worth to mention that previous studies estimating serum TCE levels in human beings were highly variable due to different populations, occupational versus non-occupational exposure and various measurement methodologies. For example, in an occupational study in the United States, TCE has been estimated in the blood of 157 metal workers who had an average concentration of 2.5 μg/L (range: 0–22 μg/L) ( Pfaffenberger et al, 1984 ). On the other hand, analysis of TCE levels in samples taken from 290 subjects revealed that the mean concentration was 0.013 μg/L besides that 88% of samples were found to be below the limit of detection ( Jia et al, 2012 ).…”

Section: Resultsmentioning

confidence: 99%

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Toxic Effects of Trichloroethylene on Rat Neuroprogenitor Cells

et al. 2018

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Trichloroethylene (TCE) is a common volatile organic solvent which is considered as an ubiquitous environmental pollutant. It is claimed to be a developmental neurotoxicant. Our group evaluated previously its impact on three-dimensional neurospheres in vitro. The current work aims to investigate the neurotoxic effects of a lower concentration of TCE on the same system. To perform the experiment, neural progenitor cells were obtained from the brains of nine newborn rats. Afterward, these cells were cultured in both growth and differentiation media to get the neurospheres. Cell cultures were divided into two groups: group 1 (control), group 2 (exposed to 0.25 μM TCE). Neurospheres were photographed at different durations and assessment of the morphological changes such as proliferation and differentiation of neurospheres was done. In addition, cell viability, apoptosis, and necrosis were analyzed using flow cytometry to clarify the mechanism of involved cytotoxicity. The results revealed that TCE-treated neurospheres showed significantly decreased proliferation on days 7 and 14. These cells failed to show the neurogenic differentiation seen in the neurospheres of the control group. Furthermore, a highly significant decrease in viability and a significant increase in the number of apoptotic cells were observed in the treated cells in comparison to the control group. The present work confirmed that TCE, at very low doses relevant to daily life exposure in humans, caused neurotoxic effects in 3D neurosphere model through the affection of neural proliferation and differentiation as well as disturbance of cell viability and apoptosis.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%