Blood Parameters and Spleen Histology Following Chronic Consumption of Ethanolic Extract of Costus afer Stem and Juice on Albino Wistar Rats

Akpantah, Amabe O.; Akpan, Ubon; Eluwa, Mokutima A.; Isamoh, T. E.; Udonkang, Mfoniso; Ekanem, Theresa B.

doi:10.9734/bjmmr/2016/23414

Cited by 8 publications

(10 citation statements)

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“…The safety of the stem has been confirmed with acute toxicity higher than 5000mg/kg b.w. of mice and chronic consumption has been reported to show no significant adverse effect on the biochemistry and histology of major organs and tissues of the body (Goodies et al, 2015;Tonkiri et al, 2015;Akpantah et al, 2016). This study was therefore carried out to investigate the morphometric and histological effects of aqueous extract of Costus afer stem juice on the cutaneous wounds of adult albino rats.…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Morphometric and Histological Studies of Effects of Aqueous Extract of Costus Afer Stem Juice on Cutaneous Wound of Albino Rats.

Ekanem¹,

Udoh²,

Peter³

2018

IJAR

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Keywords:-wound healing, Costus afer stem, regeneration of skin, dermal organization, tensile strength.The aim of this study was to investigate the morphometric and histological effects of aqueous extact of Costus afer stem on rat skin wound. Phytochemical analysis of the extract showed the presence of saponin, alkaloids, lignans, terpenes, quinones, stilbenes and flavonoids. Calcium, magnesium, potassium, copper, manganese and phosphorus were also detected in the extract. Twenty-five adult female albino rats with weights between 243-320g were used for the study. The rats were divided equally into 5 groups. They were wounded on their dorsum under ketamine hydrochloride anaesthesia. Immediately hemostasis was attained, the rats were treated according to their groups. Group A wounds were left without treatment, group B were treated only on the first day with iodine ointment, group C were treated for 7days with iodine ointment, group D were treated only on the first day with Costus afer paste, and group E were treated for 7days with Costus afer paste. Using ruler android application, the rate of contraction was not significantly different across the groups and the rate of reepithelialization was significantly highest in group D at p<0.05. the tensile strength measured with tensiometer was significantly highest (p<0.05) in group D at 692.20g. Masson's trichrome sections showed randomly organized collagen fibres in group D and E. There was also indication of growing hair follicles in group D. In conclusion, aqueous extract of Costus afer stem prove to be more effective in wound healing than iodine and its stem juice can be used cautiously for wound treatment.

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Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Morphometric and Histological Studies of Effects of Aqueous Extract of Costus Afer Stem Juice on Cutaneous Wound of Albino Rats.

Ekanem¹,

Udoh²,

Peter³

2018

IJAR

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“…The juice obtained from the blender was filtered with a chess cloth. Aqueous crude extract of the filtrate was obtained by evaporation in beakers placed in water bath at 40°C [28]. The extract was made into a semiliquid paste of 4g/ml concentration with water [25].…”

Section: Plant Collection and Preparation Of Extractmentioning

confidence: 99%

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2018

TAPI

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This study investigated the expression of e-cadherin in the epidermis of re-epithelialized wound treated with Costus afer stem juice extract. Twenty-five adult female albino rats were used for the study. They were divided equally into 5 treatment groups of: A -no treatment; B -iodine treatment only on the first day; C -iodine treatment for 7 days; D -Costus afer stem juice extract only on the first day; and E -Costus afer stem juice extract for 7 days. The rats were aseptically wounded on their dorsum under ketamine hydrochloride anaesthesia. They were treated according to their groups and closely monitored till the wounds healed. On healing, the rats were euthanized and samples of the healed skin were collected and processed for immunohistochemical staining using antibodies for e-cadherin. It was found that group D showed highest intensity of e-cadherin expression among the treatment groups, suggesting the influence of Costus afer stem juice extract on the process of mesenchymal-epithelial transition.

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“…Various parts of these plants have also been used as a remedy for adjuvant-induced polyarthritis, rheumatic pains, and as a fodder to treat goats with retained placenta [5,6]. The plant is also used to treat hypertension, worms, and hemorrhoids [7]. It has been revealed that the plant is rich in flavonoids, anthraquinones, terpenoids, steroidal saponins, oxalates, furan derivatives, and diogenin [4,5,8].…”

Section: Introductionmentioning

confidence: 99%

Paracetamol and Hydrogen peroxide-induced tissue alterations are reduced by Costus afer extract

Okoko¹

2018

Am J Res Med Sci

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Aim/Objective: To evaluate the effect of the methanolic extract of Costus afer stem on paracetamol-induced tissue injury in rats (in-vivo) and its ability to reduce hydrogen peroxide-induced erythrocyte damage (in-vitro). Methods: Twenty-five rats were acclimatized for two weeks and randomized into five groups. Group I was untreated controls, while each rat in groups III-V was given a single intraperitoneal administration of paracetamol (800 mg/kg b.w.). Prior to the administration of paracetamol, rats in groups II and IV were orally given Costus afer extract at 200 mg/kg b.w, while rats in group V were given 400 mg/kg b.w. extract once daily for four days. In the other experiment, hydrogen peroxide was used to induce erythrocyte damage (after isolating erythrocytes from whole blood) and subsequently incubated with the extract at different concentrations. Markers for tissue and erythrocyte damage were assessed in serum, tissue homogenates, and erythrocytes. Results: Paracetamol caused marked elevations (p < 0.05) in the levels of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), urea, and creatinine in serum. The levels of thiobarbituric acid reactive species (TBARS) in both the liver and the kidney were also raised when compared to untreated control (p < 0.05) while the activities of superoxide dismutase (SOD) and catalase (CAT) were reduced when compared to untreated controls (p < 0.05). Pretreatment of rats with Costus afer extract (p.o.) caused a significant reduction in the serum levels of ALT, AST, ALP, urea, creatinine, and tissue TBARS closer to the control values (p < 0.05). Pretreatment of rats with the plant extract also increased the activities of SOD and CAT in both the liver and the kidney. Apart from the levels of TBARS in the liver and CAT in the kidney, rats pretreated with 400 mg/kg b.w. extract showed greater response than in pretreatment with 200 mg/kg b.w. The extract also reduced the hydrogen peroxide-induced erythrocyte damage assessed as hemolysis and lipid peroxidation, which was concentration-dependent (p < 0.05). Conclusion: This indicated that Costus afer possesses the ability to protect the various tissues against chemical-induced injury. ARTICLE HISTORY

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Blood Parameters and Spleen Histology Following Chronic Consumption of Ethanolic Extract of Costus afer Stem and Juice on Albino Wistar Rats

Cited by 8 publications

References 10 publications

Morphometric and Histological Studies of Effects of Aqueous Extract of Costus Afer Stem Juice on Cutaneous Wound of Albino Rats.

Morphometric and Histological Studies of Effects of Aqueous Extract of Costus Afer Stem Juice on Cutaneous Wound of Albino Rats.

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Paracetamol and Hydrogen peroxide-induced tissue alterations are reduced by Costus afer extract

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