Blood Oxidative Stress Markers in Response to Aerobic Exercise

Goldfarb, Allan H.; You, T; Landes, Sabine; Murphy, Caitríona

doi:10.1097/00005768-200205001-01395

Cited by 5 publications

(9 citation statements)

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“…While PC in the present study was highest immediately postexercise following 30 minutes of cycling at approximately 73% V O 2 max, the elevation from baseline did not reach statistical significance (0.3020 Ϯ 0.0279 to 0.5180 Ϯ 0.0609 nM·mg protein Ϫ1 , mean Ϯ SEM). It is possible that aerobic exercise of a greater intensity or duration could have produced PC at greater concentrations after exercise, as shown previously by Goldfarb et al (15). It is interesting to note that the mean PC values for subjects in the present investigation were lower (0.518 nM·mg protein Ϫ1 ) than those reported by Goldfarb et al (15) in subjects following the 60% (0.60 nM·mg protein Ϫ1 ) and 80% (1.20 nM·mg protein Ϫ1 ) V O 2 max bouts.…”

Section: Discussionmentioning

confidence: 63%

“…It is possible that aerobic exercise of a greater intensity or duration could have produced PC at greater concentrations after exercise, as shown previously by Goldfarb et al (15). It is interesting to note that the mean PC values for subjects in the present investigation were lower (0.518 nM·mg protein Ϫ1 ) than those reported by Goldfarb et al (15) in subjects following the 60% (0.60 nM·mg protein Ϫ1 ) and 80% (1.20 nM·mg protein Ϫ1 ) V O 2 max bouts. Davis and coworkers (10) recently demonstrated an exercise duration effect on PC elevation following cycling exercise in trained men.…”

Section: Discussionmentioning

confidence: 63%

“…Based on these findings, which were obtained under similar cycling exercise conditions, it appears that the training status of the subjects could have impacted blood protein oxidation. That is, subjects in the present study, in addition to those in the Davis et al (10) investigation, were aerobically trained and experienced an attenuated increase in PC compared with untrained subjects in the Goldfarb et al (15) study.…”

Section: Discussionmentioning

confidence: 76%

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Effects of Acute Aerobic and Anaerobic Exercise on Blood Markers of Oxidative Stress

Goldfarb

Wideman

McKenzie

et al. 2005

Journal of Strength and Conditioning Research

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Bloomer, R.J., A.H. Goldfarb, L. Wideman, M.J. McKenzie, and L.A. Consitt. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J. Strength Cond. Res. 19(2):276-285. 2005.-The purpose of this study was to compare oxidative modification of blood proteins, lipids, DNA, and glutathione in the 24 hours following aerobic and anaerobic exercise using similar muscle groups. Ten cross-trained men (24.3 Ϯ 3.8 years, [mean Ϯ SEM]) performed in random order 30 minutes of continuous cycling at 70% of V O 2 max and intermittent dumbbell squatting at 70% of 1 repetition maximum (1RM), separated by 1-2 weeks, in a crossover design. Blood samples taken before, and immediately, 1, 6, and 24 hours postexercise were analyzed for plasma protein carbonyls (PC), plasma malondialdehyde (MDA), and whole-blood total (TGSH), oxidized (GSSG), and reduced (GSH) glutathione. Blood samples taken before and 24 hours postexercise were analyzed for serum 8-hydroxy-2Ј-deoxyguanosine (8-OHdG). PC values were greater at 6 and 24 hours postexercise compared with pre-exercise for squatting, with greater PC values at 24 hours postexercise for squatting compared with cycling (0.634 Ϯ 0.053 vs. 0.359 Ϯ 0.018 nM·mg protein Ϫ1 ). There was no significant interaction or main effects for MDA or 8-OHdG. GSSG experienced a shortlived increase and GSH a transient decrease immediately following both exercise modes. These data suggest that 30 minutes of aerobic and anaerobic exercise performed by young, crosstrained men (a) can increase certain biomarkers of oxidative stress in blood, (b) differentially affect oxidative stress biomarkers, and (c) result in a different magnitude of oxidation based on the macromolecule studied. Practical applications: While protein and glutathione oxidation was increased following acute exercise as performed in this study, future research may investigate methods of reducing macromolecule oxidation, possibly through the use of antioxidant therapy.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 76%

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Effects of Acute Aerobic and Anaerobic Exercise on Blood Markers of Oxidative Stress

Goldfarb

Wideman

McKenzie

et al. 2005

Journal of Strength and Conditioning Research

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“…To our knowledge, this was the first study to compare protein oxidation between men and women following different exercise durations. Only one study presented in abstract form has compared protein oxidation following aerobic exercise with regards to sex [5]. In the study by Goldfarb et al [5], subjects cycled for 30 minutes at both 60 and 80% V O 2max .…”

Section: Discussionmentioning

confidence: 99%

“…Little is known regarding the impact of exercise duration pertaining to protein oxidation. To our knowledge, only Goldfarb et al [5] has compared protein oxidation resulting from aerobic exercise between men and women. In this study, protein carbonyls were noted to be elevated following 30 minutes of cycling at both 60 % and 80 % V O 2max in both men and women, with no differences noted between sexes.…”

Section: Introductionmentioning

confidence: 99%

Plasma Protein Carbonyl Response to Increasing Exercise Duration in Aerobically Trained Men and Women

et al. 2007

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Abstract:The purpose of this study was to determine the effect of aerobic exercise duration on plasma protein carbonyl concentrations, a marker of protein oxidation, in aerobically trained men and women. Eight men (age: 27 ± 4 years, V·O 2peak : 4.09 ± 0.26 L · min -1 ; mean ± SD) and 7 women (age: 27 ± 6 years, V·O 2peak : 2.33 ± 0.24 L · min -1 ) exercised on an electrically-braked cycle ergometer at 70 % V·O 2peak for 30, 60 or 120 minutes on three separate days. Plasma samples collected before and immediately, 30-and 60-minutes post-exercise were analyzed for protein carbonyls. Mean oxygen uptake was greater for men in all conditions (2.75 ± 0.03 L · min -1 ; 38 ± 0.43 ml · kg -1 · min -1 ) compared to women (1.57 ± 0.03 L · min -1 ; 24.1 ± 0.47 ml · kg -1 · min -1 ). Total work performed during the exercise sessions was also greater for men than for women during the 30 (368 ± 11 versus 223 ± 7 kJ), 60 (697 ± 17 versus 423 ± 18 kJ), and 120-minute conditions (1173 ± 44 versus 726 ± 28 kJ) (Mean ± SEM). Although these comparisons were significant (p < 0.0001), sex differences in total work performed and mean V·O 2 did not result in sex differences in protein carbonyls. However, a condition by time interaction was observed with greater post-exercise values following the 120-minute condition compared to both the 30-and 60-minute conditions. Protein carbonyl concentration was greatest immediately post-exercise for both men and women and generally declined in a linear trend through one hour of recovery. These data suggest that protein carbonyl concentration is elevated by cycling exercise performed at 70 % V·O 2peak , is greater following longer duration rides, begins to recover within one hour following exercise, and is not different between men and women.

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Flight muscle protein damage during endurance flight is related to energy expenditure but not dietary polyunsaturated fatty acids in a migratory bird

Dick

Guglielmo

2019

Journal of Experimental Biology

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Migration poses many physiological challenges for birds, including sustaining high intensity aerobic exercise for hours or days. A consequence of endurance flight is the production of reactive oxygen species (ROS). ROS production may be influenced by dietary polyunsaturated fatty acids (PUFA), which, although prone to oxidative damage, may limit mitochondrial ROS production and increase antioxidant capacity. We examined how flight muscles manage oxidative stress during flight, and whether dietary long-chain PUFA influence ROS management or damage. Yellow-rumped warblers were fed diets low in PUFA, or high in long-chain n-3 or n-6 PUFA. Flight muscle was sampled from birds in each diet treatment at rest or immediately after flying for up to a maximum of 360 min in a wind tunnel. Flight increased flight muscle superoxide dismutase activity but had no effect on catalase activity. The ratio of glutathione to glutathione disulphide decreased during flight. Oxidative protein damage, indicated by protein carbonyls, increased with flight duration (Pearson r=0.4). Further examination of just individuals that flew for 360 min (N=15) indicates that oxidative damage was related more to total energy expenditure (Pearson r=0.86) than to flight duration itself. This suggests that high quality individuals with higher flight efficiency have not only lower energy costs but also potentially less oxidative damage to repair after arrival at the destination. No significant effects of dietary long-chain PUFA were observed on antioxidants or damage. Overall, flight results in oxidative stress and the degree of damage is likely driven more by energy costs than fatty acid nutrition.

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Blood Oxidative Stress Markers in Response to Aerobic Exercise

Cited by 5 publications

References 0 publications

Effects of Acute Aerobic and Anaerobic Exercise on Blood Markers of Oxidative Stress

Effects of Acute Aerobic and Anaerobic Exercise on Blood Markers of Oxidative Stress

Plasma Protein Carbonyl Response to Increasing Exercise Duration in Aerobically Trained Men and Women

Flight muscle protein damage during endurance flight is related to energy expenditure but not dietary polyunsaturated fatty acids in a migratory bird

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