Blood monocyte concentration is critical for enhancement of collateral artery growth

Heil, Matthias; Ziegelhoeffer, Tibor; Pipp, Frederic; Kostin, Sawa; Martin, Sarah E.; Clauss, Matthias; Schäper, Wolfgang

doi:10.1152/ajpheart.01098.2001

Cited by 260 publications

(248 citation statements)

References 37 publications

Supporting

Mentioning

224

Contrasting

Unclassified

Order By: Relevance

“…This finding has been confirmed by recent studies, which demonstrate that monocyte recruitment is central to postnatal blood vessel formation [92][93][94]. This mechanism has, in part, been attributed to the critical role of VEGF in adult neovascularization, and prevention of neovessel regression [95][96][97].…”

Section: Decellularized Tissue-derived Matrices: the Biologic Countersupporting

confidence: 63%

Tissue engineering on matrix: future of autologous tissue replacement

et al. 2011

View full text Add to dashboard Cite

show abstract

Section: Decellularized Tissue-derived Matrices: the Biologic Countersupporting

confidence: 63%

Tissue engineering on matrix: future of autologous tissue replacement

et al. 2011

View full text Add to dashboard Cite

show abstract

“…3 A and B). Rapid early monocyte recruitment has been shown to be intricately involved in postnatal blood vessel formation (32)(33)(34). In particular, successful arteriogenesis is dependent on MCP-1-induced monocyte recruitment to preexistent collateral arterioles (12)(13)(14)(15)35).…”

Section: Expression) (C-e) Luminex Assaymentioning

confidence: 99%

Tissue-engineered vascular grafts transform into mature blood vessels via an inflammation-mediated process of vascular remodeling

Roh

Sawh-Martinez

Brennan

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

495

492

View full text Add to dashboard Cite

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are the earliest tissue-engineered vascular grafts (TEVGs) to be used clinically. These TEVGs transform into living blood vessels in vivo, with an endothelial cell (EC) lining invested by smooth muscle cells (SMCs); however, the process by which this occurs is unclear. To test if the seeded BMCs differentiate into the mature vascular cells of the neovessel, we implanted an immunodeficient mouse recipient with human BMC (hBMC)-seeded scaffolds. As in humans, TEVGs implanted in a mouse host as venous interposition grafts gradually transformed into living blood vessels over a 6-month time course. Seeded hBMCs, however, were no longer detectable within a few days of implantation. Instead, scaffolds were initially repopulated by mouse monocytes and subsequently repopulated by mouse SMCs and ECs. Seeded BMCs secreted significant amounts of monocyte chemoattractant protein-1 and increased early monocyte recruitment. These findings suggest TEVGs transform into functional neovessels via an inflammatory process of vascular remodeling.bone marrow | monocyte chemoattractant protein-1 | tissue engineering | neovascularization C ongenital heart disease is a leading cause of infant mortality, often requiring early surgical intervention to correct fatal cardiovascular malformations. Prosthetic vascular grafts are widely used in these reconstructive operations, but revisions are often necessary because of their inability to grow or effectively remodel within a growing child (1-3). A strategy to address this issue is the use of living tissue-engineered vascular grafts (TEVGs). Constructed from biodegradable polyester tubes seeded with autologous bone marrow mononuclear cells (BMCs), these grafts undergo extensive remodeling in animal recipients and appear to transform into living blood vessels, similar in morphology and function to the native veins into which they are interposed (4, 5). Ongoing clinical studies evaluating BMC-seeded grafts as venous conduits for congenital heart surgery report excellent safety profiles and 100% patency rates at 1-3 years of follow-up (6-8). Additionally, these grafts demonstrate growth potential, suggesting they may be more effective for the pediatric patient population than currently available vascular grafts (8,9).Although the functional efficacy and clinical utility of TEVGs are promising, little is known about how these BMC-seeded polyester tubes transform into living blood vessels in host recipients. It has been proposed that stem cells within the seeded BMC population differentiate into the endothelial cells (ECs) and smooth muscle cells (SMCs) of the developing neovessel, ultimately replacing the degrading polyester tube (10). This hypothesis, however, has not been directly examined.We recently developed a method for constructing small-diameter biodegradable synthetic scaffolds suitable for use as vascular grafts in mice (11). These tubular scaffolds are composed of the same materials and design used in clinical TEVGs...

show abstract

“…In another subgroup comprising 9 active-and 6 inactive-RA patients as well as 6 HRs, CACs, known to be derived mainly from the monocyte/leukocyte lineage 24 and attributed with proangiogenic effects, 32 were quantified by culture assay. In brief, PBMCs were isolated by density gradient centrifugation on Ficoll-Paque.…”

Section: Cac Culture Assaymentioning

confidence: 99%

Depletion of Endothelial Progenitor Cells in the Peripheral Blood of Patients With Rheumatoid Arthritis

et al. 2005

View full text Add to dashboard Cite

Background-Rheumatoid arthritis (RA) is characterized by increased cardiovascular morbidity and mortality that cannot be explained solely by traditional cardiovascular risk factors. Cardiovascular morbidity is related to disease activity and can be normalized by effective therapy. Because the quantity of endothelial progenitor cells (EPCs) in the peripheral blood is correlated inversely with cardiovascular risk, we studied whether such abnormalities could also be observed in patients with RA. Methods and Results-EPCs were determined in 52 RA patients and in 16 healthy referents (HRs) by fluorescence-activated cell-sorting (FACS) analysis. Patients were divided into groups characterized by active disease (nϭ36) and low disease activity (nϭ16). Cells that were positive by flow cytometry for CD34/KDR/AC133 within the lymphocyte population were characterized as EPCs. Furthermore, in subgroups of patients, circulating EPCs were also quantified by a colony-forming unit (CFU) and a circulating angiogenic cell (CAC) assay.

show abstract

Blood monocyte concentration is critical for enhancement of collateral artery growth

Cited by 260 publications

References 37 publications

Tissue engineering on matrix: future of autologous tissue replacement

Tissue engineering on matrix: future of autologous tissue replacement

Tissue-engineered vascular grafts transform into mature blood vessels via an inflammation-mediated process of vascular remodeling

Depletion of Endothelial Progenitor Cells in the Peripheral Blood of Patients With Rheumatoid Arthritis

Contact Info

Product

Resources

About