Blood micronutrients and DNA damage in children

Milne, Elizabeth; Greenop, Kathryn R.; Ramankutty, P.; Miller, Margaret; Klerk, Nicholas de; Armstrong, Bruce K.; Almond, Theodora; O’Callaghan, Nathan; Fenech, Michael

doi:10.1002/mnfr.201500110

Cited by 8 publications

(6 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar scenarios have also been observed for deficiencies in vitamin B-12, riboflavin, vitamin A, and minerals such as calcium and zinc (99). Conversely, supplementation with folic acid (97, 100-108), riboflavin, pantothenic acid, biotin (4), cobalamin (93,103), vitamin C (109)(110)(111)(112)(113)(114)(115), vitamin E (114), and vitamin A and its derivatives (107,112) was associated with decreased genotoxic damage and micronucleus reduction (4). Much research has been conducted to show the effects of folic acid supplementation and folate status on reduced micronucleus frequency.…”

Section: Micronutrients and Chromatin Instabilitymentioning

confidence: 61%

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Hernández-Saavedra

Strakovsky

Ostrosky‐Wegman

et al. 2017

Advances in Nutrition

View full text Add to dashboard Cite

The centromere is a genomic locus required for the segregation of the chromosomes during cell division. This chromosomal region together with pericentromeres has been found to be susceptible to damage, and thus the perturbation of the centromere could lead to the development of aneuploidic events. Metabolic abnormalities that underlie the generation of cancer include inflammation, oxidative stress, cell cycle deregulation, and numerous others. The micronucleus assay, an early clinical marker of cancer, has been shown to provide a reliable measure of genotoxic damage that may signal cancer initiation. In the current review, we will discuss the events that lead to micronucleus formation and centromeric and pericentromeric chromatin instability, as well transcripts emanating from these regions, which were previously thought to be inactive. Studies were selected in PubMed if they reported the effects of nutritional status (macro- and micronutrients) or environmental toxicant exposure on micronucleus frequency or any other chromosomal abnormality in humans, animals, or cell models. Mounting evidence from epidemiologic, environmental, and nutritional studies provides a novel perspective on the origination of aneuploidic events. Although substantial evidence exists describing the role that nutritional status and environmental toxicants have on the generation of micronuclei and other nuclear aberrations, limited information is available to describe the importance of macro- and micronutrients on centromeric and pericentromeric chromatin stability. Moving forward, studies that specifically address the direct link between nutritional status, excess, or deficiency and the epigenetic regulation of the centromere will provide much needed insight into the nutritional and environmental regulation of this chromosomal region and the initiation of aneuploidy.

show abstract

Section: Micronutrients and Chromatin Instabilitymentioning

confidence: 61%

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Hernández-Saavedra

Strakovsky

Ostrosky‐Wegman

et al. 2017

Advances in Nutrition

View full text Add to dashboard Cite

show abstract

“…By contrast, another study evaluating the effect of supplementation with 100 μM Zn sulfate showed that human lymphocyte viability was not affected (Sliwinski et al 2009). Elevated MNi frequency in lymphocytes has been shown to be associated prospectively with an increased risk of cancer in a cohort study, with severe adverse cardiovascular events in coronary artery disease patients and with mortality from cancer or cardiovascular disease in a case-control study (Milne et al 2015).…”

Section: Discussionmentioning

confidence: 94%

Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?

Padula

Ponzinibbio

Gambaro

et al. 2017

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Micronutrients are important for the prevention of degenerative diseases due to their role in maintaining genomic stability. Therefore, there is international concern about the need to redefine the optimal mineral and vitamin requirements to prevent DNA damage. We analyzed the cytostatic, cytotoxic, and genotoxic effect of in vitro zinc supplementation to determine the effects of zinc deficiency and excess and whether the upper estimate of the physiological requirement recommended for children is safe. To achieve zinc deficiency, DMEM/Ham's F12 medium (HF12) was chelated (HF12Q). Lymphocytes were isolated from healthy female donors (age range, 5-10 yr) and cultured for 7 d as follows: negative control (HF12, 60 μg/dl ZnSO); deficient (HF12Q, 12 μg/dl ZnSO); lower level (HF12Q + 80 μg/dl ZnSO); average level (HF12Q + 180 μg/dl ZnSO); upper limit (HF12Q + 280 μg/dl ZnSO); and excess (HF12Q + 380 μg/dl ZnSO). The comet (quantitative analysis) and cytokinesis-block micronucleus cytome assays were used. Differences were evaluated with Kruskal-Wallis and ANOVA (p < 0.05). Olive tail moment, tail length, micronuclei frequency, and apoptotic and necrotic percentages were significantly higher in the deficient, upper limit, and excess cultures compared with the negative control, lower, and average limit ones. In vitro zinc supplementation at the lower and average limit (80 and 180 μg/dl ZnSO) of the physiological requirement recommended for children proved to be the most beneficial in avoiding genomic instability, whereas the deficient, upper limit, and excess (12, 280, and 380 μg/dl) cultures increased DNA and chromosomal damage and apoptotic and necrotic frequencies.

show abstract

“…For the study we chose method for blood cell cultivation with cytochalasine B usually used for detection of micronuclei in binuclear cells [16]. In this study we wide the ability of the method, taking into consideration, practically, all types of cells, micronuclei in them, apoptosis and mitotic activity ( Figure 4).…”

Section: Human Emotional Stress and Environmental Pollutionmentioning

confidence: 99%

“…This experimental approach is directed mainly to the evaluation of the activity of apoptosis, because the role of the low-priming-dose of ionizing radiation, which itself, practically, doesn't induce detectable levels of genetic damage, is, mostly, to induce apoptosis [10]- [16].…”

Section: Human Emotional Stress and Environmental Pollutionmentioning

confidence: 99%

See 1 more Smart Citation

Children’s, Adult’s and Family's Emotional Stress in Context of Genomic Instability

Ingel¹,

Krivtsova²,

Urtseva³

et al. 2018

JSS

View full text Add to dashboard Cite

Scientific data concerning the impact of emotional stress to human genomic instability very seldom describes in literature. For many scientists this connection is not supposed to be obvious, although oncologists and psychologists know that a prolonged state of heightened emotional tension is fraught with serious problems for the nervous-immune-endocrine system of the organism. Moreover, oncologists know that cancer is often the result of resentment and loneliness. At the same time, the role of genome instability in processes of tumor induction and progression is proved very correctly. In the report will be paying attention to 3 aspects of human life in context of emotional stress expression and its connection with genomic instability: environmental pollution, genomic predisposes, ethic and social-economic problems. The report will contain data from literature and results of own research directed to the analyzing the impact of the degree of emotional stress expression on the children's and adults' genomic instability. Special attention will be paid to the investigation how emotional state of parents and teachers impact on young children's genomic instability. Methods. For evaluation of stress expression levels we used the complex of standard psychological tests: questionnairesfor adults and 8-coloured M. Luscher's test-for children. Estimation of genomic instability was carried out in blood cultures by test on chromosome aberration and micronuclei test with Cytochalasin B. Alteration of biochemical indices were detected by standard methods.

show abstract

Blood micronutrients and DNA damage in children

Abstract: DNA damage in healthy children may be influenced by blood micronutrient levels and certain genotypes. Further investigation of associations between nutritional status and genomic integrity in children is needed to shed additional light on potential mechanisms.

Cited by 8 publications

References 77 publications

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?

Children’s, Adult’s and Family's Emotional Stress in Context of Genomic Instability

Contact Info

Product

Resources

About

Blood micronutrients and DNA damage in children

Abstract: DNA damage in healthy children may be influenced by blood micronutrient levels and certain genotypes. Further investigation of associations between nutritional status and genomic integrity in children is needed to shed additional light on potential mechanisms.

Cited by 8 publications

References 77 publications

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Epigenetic Regulation of Centromere Chromatin Stability by Dietary and Environmental Factors

Genomic instability related to zinc deficiency and excess in an in vitro model: is the upper estimate of the physiological requirements recommended for children safe?

Children’s, Adult’s and Family&#39;s Emotional Stress in Context of Genomic Instability

Contact Info

Product

Resources

About

Children’s, Adult’s and Family's Emotional Stress in Context of Genomic Instability