Blood meal drives de novo lipogenesis in the fat body of Rhodnius prolixus

Saraiva, Felipe B.; Auger, Michèle; Majerowicz, David; Paes-Vieira, Lisvane; Braz, Valdir; Almeida, Muriel G.M.D.; Meyer‐Fernandes, José Roberto; Gondim, Kátia C.

doi:10.1016/j.ibmb.2020.103511

Cited by 14 publications

(22 citation statements)

References 62 publications

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“…The dynamic coordination of gene expression may be a key process to regulate lipid metabolism in sunn pest. Saraiva et al (2021) investigated the regulation of Rhodnius prolixus ACC (RhoprACC) expression and de novo lipogenesis activity in adult females under different nutritional and metabolic conditions. In the fat body, gene expression increased in fasted females, and it decreased after a blood meal.…”

Section: Discussionmentioning

confidence: 99%

Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.

et al. 2021

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The ladybird Coccinella septempunctata L., a predatory insect, serves as an excellent biological control agent against common agricultural pests. It undergoes a diapause phenomenon, during which a large amount of fat accumulates in the abdomen. A comprehensive analysis of this lipid accumulation can reveal the molecular mechanisms underlying diapause regulation, which can be exploited to improve the shipping and transport of the insect for agricultural applications. In this study, we compared the transcriptome of C. septempunctata during non-diapause, diapause, and post-diapause and screened four key genes related to lipid metabolism. The cDNA of these four relevant enzymes, acetyl-CoA carboxylase (ACC), long-chain fatty acid-CoA ligase (ACSL), elongase of very-long-chain fatty acids (ELO), and very-long-chain 3-oxoacyl-CoA reductase (KAR), were cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Their expression profiles were analyzed during the preparation and maintenance phases of diapause and the post-diapause phase. The functions of these four key enzymes in diapause were further verified using RNA interference. All four genes were most closely related to the homeotic gene from Tribolium castaneum. The expression profiles of these four genes were significantly affected under diapause-inducing conditions; their expression level was the highest in the diapause preparation phase, and it gradually decreased with the diapause induction time. RNA interference showed that the target genes play important roles in fat storage during early diapause, and the decrease in their expression leads to a decrease in lipid content in C. septempunctata. These results indicate an important role of ACC, ACSL, ELO, and KAR in lipid accumulation. Our findings could help elucidate the production and accumulation of lipids by insects during the preparation for diapause and improve biological control.

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Section: Discussionmentioning

confidence: 99%

Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.

et al. 2021

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“…Thus, hematophagous insects rely on amino acids derived from blood proteins to synthesize lipids (Gondim et al, 2018). Moreover, as these insects usually feed on large and infrequent meals, and it may take a long time before the next meal, lipid synthesis is finely regulated, and in R. prolixus, it is highly activated soon after feeding (Saraiva et al, 2021). In the de novo fatty acid synthesis pathway, the first step is catalyzed by the enzyme acetyl-CoA carboxylase (ACC), where acetyl-CoA is converted to malonyl-CoA that is a substrate for fatty acid synthase (FASN).…”

Section: Introductionmentioning

confidence: 99%

“…In R. prolixus, after feeding, Lp transports diacylglycerol, fatty acids, phospholipids, and cholesterol to the vitellogenic ovaries (Gondim et al, 1989;Santos et al, 2011;Entringer et al, 2021). These lipids are loaded onto circulating Lp at the midgut during blood digestion, but they may also be acquired from the fat body, where-except cholesterol-they may result from de novo lipogenesis (Saraiva et al, 2021). In this insect, lipid synthesis by the fat body is triggered by the blood meal, and amino acids released by the digestion of blood proteins are used as substrates (Saraiva et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…These lipids are loaded onto circulating Lp at the midgut during blood digestion, but they may also be acquired from the fat body, where-except cholesterol-they may result from de novo lipogenesis (Saraiva et al, 2021). In this insect, lipid synthesis by the fat body is triggered by the blood meal, and amino acids released by the digestion of blood proteins are used as substrates (Saraiva et al, 2021). Unlike vertebrates, insects and all other arthropods have only one copy of the ACC gene (Saraiva et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Deficiency of Acetyl-CoA Carboxylase Impairs Digestion, Lipid Synthesis, and Reproduction in the Kissing Bug Rhodnius prolixus

et al. 2022

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Rhodnius prolixus is a hematophagous insect, vector of Chagas disease. After feeding, as blood is slowly digested, amino acids are used as substrates to fuel lipid synthesis, and adult females accumulate lipids in the fat body and produce eggs. In order to evaluate the importance of de novo fatty acid synthesis for this insect metabolism, we generated acetyl-CoA carboxylase (ACC) deficient insects. The knockdown (AccKD) females had delayed blood digestion and a shorter lifespan. Their fat bodies showed reduced de novo lipogenesis activity, did not accumulate triacylglycerol during the days after blood meal, and had smaller lipid droplets. At 10 days after feeding, there was a general decrease in the amounts of neutral lipids and phospholipids in the fat body. In the hemolymph, no difference was observed in lipid composition at 5 days after blood meal, but at day ten, there was an increase in hydrocarbon content and a decrease in phospholipids. Total protein concentration and amino acid composition were not affected. The AccKD females laid 60% fewer eggs than the control ones, and only 7% hatched (89% for control), although their total protein and triacylglycerol contents were not different. Scanning electron microscopy of the egg surface showed that chorion (eggshell) from the eggs laid by the AccKD insects had an altered ultrastructural pattern when compared to control ones. These results show that ACC has a central role in R. prolixus nutrient homeostasis, and its appropriate activity is important to digestion, lipid synthesis and storage, and reproductive success.

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“…The utilization rate of chicken blood is very low, although it is rich in nutrients. Most animal blood, especially chicken blood, is mostly processed into low-value animal primary feed or blood curds employed as a cheap source of protein and iron in Asian diets [6] .…”

Section: Introductionmentioning

confidence: 99%

Effect of ultrasound assisted konjac glucomannan treatment on properties of chicken plasma protein gelation

Zou

Yang

et al. 2021

Ultrasonics Sonochemistry

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Blood meal drives de novo lipogenesis in the fat body of Rhodnius prolixus

Cited by 14 publications

References 62 publications

Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.

Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.

Deficiency of Acetyl-CoA Carboxylase Impairs Digestion, Lipid Synthesis, and Reproduction in the Kissing Bug Rhodnius prolixus

Effect of ultrasound assisted konjac glucomannan treatment on properties of chicken plasma protein gelation

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