Blood lactate minimum of rats during swimming test using three incremental stages

Abstract

The purpose of this study was to determine the lactate minimum intensity (LMI) by swimming LACmintest using three incremental stages (LACmintest3) and to evaluate its sensitivity to changes in aerobic fitness (AF). Twenty Wistar rats performed: LACmintest3 (1): induction of hyperlactacidemia and incremental phase (4%, 5% and 6.5% of bw); Constant loads tests on (2) and above (3) the LMI. Half of the animals were subjected to training with the individual LMI and the tests were performed again. The mean exercise load in LACmintest3 was 5.04 ± 0.13% bw at 5.08 ± 0.55 mmol L-1 blood lactate minimum (BLM). There was a stabilize and disproportionate increase of blood lactate in tests 2 and 3, respectively. After the training period, the mean BLM was lower in the trained animals. The LACmintest3 seems to be a good indicator of LMI and responsive to changes in AF in rats subjected to swim training.

The Lactate Minimum Test: Concept, Methodological Aspects and Insights for Future Investigations in Human and Animal Models

“…Additionally, several investigations involving animal models of different backgrounds were published. Some examples include methodological analyses (de Araujo et al, 2007 ; Beck et al, 2012 ; Sena et al, 2015 ), effects of periodized or non-periodized training (de Oliveira et al, 2007 ; de Araujo et al, 2012 , 2013a , 2016 ; Scariot et al, 2016 ) effects of luminosity and time of day (Beck and Gobatto, 2013 , 2016 ; Beck et al, 2014 ; Beck et al, 2014a , b ) and comparion with the MLSS protocol (Rodrigues et al, 2016 ).…”

Heteropterys tomentosa A. Juss: Toxicological and adaptogenic effects in experimental models

“…Male albino rats ( n = 20; 85 ± 10 days old), of the Rattus norvegicus strain, Wistar variety (weighing 301.4 ± 2.3 g), were used for the progressive loading test. The animals were randomly distributed into four groups ( n = 5 animals/group): vehicle and HEHt (25, 100 and 400 mg/kg) after 5 days of adaptation to the liquid medium and rest for 2 days before the start of the assay (Sena et al, 2015). The objective of the adaptation was to reduce the stress from exercise performed in water (Gobatto et al, 2001).…”