Eight populations in the southern part of the Korean peninsula were studied to elucidate their genetic structure and to understand the genetic relationships between them. For this purpose, several blood groups and other genetic markers were analyzed. In total, over 9,000 blood specimens were collected. About 2,000 specimens were tested for 9 blood groups.The gene frequencies were estimated as A = 0.2555, B = 0.2244,·= 0.5201, M = 0.5263, 5 = 0.0560, d = 0.0472, C = 0.6542, E = 0.3126, Fya = 0.9210, Jka = 0.4386, DP = 0.0539, P1 = 0.1939, K = 0.0010, Le = 0.6866 and Se = 0.6311 in the total sample. A continuous variation was observed for the frequency of gene B.