Blood Group Antibody Screening Tests during Pregnancy

Bowell, P. J.; Allen, David L.; Entwistle, C. C.

doi:10.1097/00006254-198707000-00006

Cited by 20 publications

(30 citation statements)

References 4 publications

(5 reference statements)

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“…In the Western population, alloimmunization against red cell blood group antigens occurs in about 1% of pregnancies (Bowell et al, 1986). Anti-D, anti-Kell and anti-c are the antibodies most often implicated in the causation of moderate to severe HDN and account for about 40% of the antibodies detected in pregnant women (Bowell et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Anti-D, anti-Kell and anti-c are the antibodies most often implicated in the causation of moderate to severe HDN and account for about 40% of the antibodies detected in pregnant women (Bowell et al, 1986). Although Rh D typing and anti-D prophylaxis have resulted in a reduction of fetal loss and neonatal death due to HDN, alloimmunization due to other antigens such as Kell and c continues to be important (MacKenzie et al, 1992 Abbreviations: G, gravida; P, parity; NSD, normal spontaneous delivery; Hb, haemoglobin; DAT, direct antiglobulin test; rbc, red blood cell; HDN, haemolytic disease of the newborn; SLL, small lymphocytic lymphoma.…”

Section: Discussionmentioning

confidence: 99%

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Is antenatal antibody screening worthwhile in Chinese?

Wong¹,

Tse

Lee³

et al. 1997

Br J Haematol

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A total of 1997 pregnant women were screened during their first antenatal visit for irregular antibodies for the prevention of haemolytic disease of the newborn. Patient sera were tested against a panel of group O screen cells including one with the expression of Miltenberger determinants GP.Mur. 17 women (0.85%) had irregular antibodies of which four were of potential clinical significance, including one with anti‐D, two with anti‐E and one with anti‐D, anti‐E and anti‐G. Although antenatal antibody screening is mandatory in Western populations, our results suggest that this may not be necessary in the Chinese population except for those who are Rh D‐negative or who have a history of haemolytic disease of the newborn.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Is antenatal antibody screening worthwhile in Chinese?

Wong¹,

Tse

Lee³

et al. 1997

Br J Haematol

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“…In the province of Tirol Schönitzer [5] discovered a prevalence of relevant antibodies of 0.58% between 1971 and 1985. For the region of Oxford Bowell et al [6] described a prevalence of antibodies among pregnant women of 0.98% between 1984 and 1985. This variability of the prevalence might be caused by regional differences; on the other hand, the higher prevalence in Salzburg might also be due to the higher sensitivity of the ABST since the introduction of gel centrifugation techniques.…”

Section: Discussionmentioning

confidence: 99%

Prevalence Rate of Red Blood Cell Antibodies during Gravidity in the Federal Province of Salzburg

Mühlbacher¹,

Michl²

1999

Transfus Med Hemother

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Background: One of the aims of maternity care is the timely recognition of the risk of hemolytic disease of the newborn (HDN) by specific screening for antibodies. Although the antibody screening test (ABST) is recommended as an obligatory measure during gravidity in the draft of the Austrian guidelines for blood group serology and transfusion medicine part IV, the Austrian ordinance concerning the mother-child screening card provides for merely a confirmation of the blood group, though no screening for antibodies. Material and Methods:Over a period of 46 months (January 1, 1994 to October 31, 1997) the ABSTs of 24,538 (19,285 D-positive and 5,253 D-negative) pregnancies were evaluated in the province of Salzburg. Results:The prevalence of antibodies in pregnancies of D-positive women amounted to a total of 0.47%, that of D-negative women to 2.41%. HDNs being in need of transfusion were found in 0.25% of the children with D-negative and in 0.02% of children with D-positive mothers.

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“…RhD(-) women are then retested during the second and third trimes ters, in contrast to RhD(+) women. In a survey of pregnant women, Bowell et al [13] have shown that 66% of those producing antibodies during pregnancy were RhD(+). In our present study, after excluding the RhIG, 71% of those producing antibodies were RhD(+).…”

Section: The Sph Screening Testmentioning

confidence: 99%

Evaluation of a Solid-Phase Test for Erythrocyte Antibody Screening of Pregnant Women, Patients and Blood Donors

Sallander¹,

Shanwell²,

Âqvist³

1996

Vox Sanguinis

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We have studied a previously described solid-phase test (SPI 1) for screening and identification of antibodies directed against erythrocyte antigens, in large sample numbers and throughout routine processing. The screening results of all samples from pregnant women, patients and blood donors (n = 36,701) sent to our blood bank from August 1992 to July 1993 were compiled and evaluated. Pregnant women were screened by the SPH-antiglobulin technique (1AT) and the SPH-enzyme-enhanced technique (ENZ), while the patients and blood donors were screened only by the SPH-IAT. Samples with known alloimmunization were excluded from this study. Positive screening reactions were further investigated by the SPH-IAT and SPH-ENZ techniques, the manual hemagglutination tests low ionic-strength solution IAT and 2-stage papain. Pregnant women: A positive reaction in one or both of the SPH tests was found in 1.2% of the samples. The SPH tests identified 99 (98.0%) and the manual methods 79 (78.2%) of a total of 101 antibodies. Of the identified antibodies, 61 were to antigens in the Rh blood group system, including 34 Rh immune globulin. The remainder had specificity directed against antigens K, Jk(a), M, S, Le(a) and Le(b). Patients: A positive reaction in the SPH-IAT test occurred in 0.9% of the samples. The antibodies identified had specificity directed against antigens D, C, c and E in the Rh blood group system. The remainder had specificity directed against antigens Fy(a), K, Jk(a), M and Le(a). All antibodies identified by SPH-IAT, except 1 anti-M and 2 anti-Le(a), were also identified by manual methods. Blood donors: A positive reaction in the SPH-IAT test was noted in 0.7% of the samples. The identified antibodies had specificity directed against antigens D, C, E, Fy(a), K and M. All antibodies identified by the SPH-IAT, except 1 anti-D found in a sample from an RhD(+) male, were also identified by the manual methods. This study indicates that the SPH-IAT test is sensitive for detecting anti-M. This was also indicated for the SPH-ENZ test concerning the Jk(a) antibody. An advantage of using solid-phase antibody screening tests for a larger series of samples is the possibility of automated sample identification, dilution, dispensing and interpretation of results. The SPH-IAT and the SPH-ENZ tests permitted us to select test cells, preferably from our own donor pool. We conclude that the SPH-IAT and SPHENZ tests are suitable for routine antibody screening of pregnant women and the SPH-IAT test for screening patients and blood donors.

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Blood Group Antibody Screening Tests during Pregnancy

Cited by 20 publications

References 4 publications

Is antenatal antibody screening worthwhile in Chinese?

Is antenatal antibody screening worthwhile in Chinese?

Prevalence Rate of Red Blood Cell Antibodies during Gravidity in the Federal Province of Salzburg

Evaluation of a Solid-Phase Test for Erythrocyte Antibody Screening of Pregnant Women, Patients and Blood Donors

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