Blood glucose determination with the reduction of resazurin as a fluorometric indicator reaction.

Directed Evolution of Cellobiose Dehydrogenase on the Surface of Yeast Cells Using Resazurin-Based Fluorescent Assay

“…Optimal concentration of resazurin dye was determined to be 0.1 mM since at higher concentrations it behaves as an inhibitor of CDH activity (Figure 2). Drop in fluorescence signal produced by enzymatic action at higher concentrations of resazurin (above 0.025 mM) was also previously reported for diaphorase coupled detection of glucose [35]. Drop in the enzyme activity at higher resazurin concentrations were results of substrate inhibition phenomenon that is often caused by nonproductive binding of the substrate for the enzyme active site.…”

“…Therefore, we tested fluorogenic resazurin dye as a substrate for detection of CDH activity on the surface of yeast cells. Resazurin reduction to resorufin that can be measured by red fluorescence was used previously for measuring viability of living cells [24] and blood glucose concentration [35]. The CDH assay using resazurin was optimized with respect to resazurin, cellobiose/lactose concentration, and pH of the reaction medium.…”

Spectrofluorometric Determination of Uric Acid and Glucose by Use of Fe(III)-Thiacalix[4]arenetetrasulfonate as a Peroxidase Mimic

“…To date, spectrofluorometric methods using various enzymes have been developed for the determination of uric acid 6) and glucose. [7][8][9][10] However, no report has been published on the spectrofluorometric method using a peroxidase mimic such as Fe 3+ -TCAS [4] A-500 .…”

Synthesis of a new long-wavelength latent fluorimetric indicator for analytes determination in the DT-Diaphorase coupling dehydrogenase assay system

“…Although redox indicators that directly or via a mediator accept an electron from an oxidizing enzyme instead of oxygen are preferred, they also have some problems. For example, resazurin, transition metal Os and Ru complexes are oxygen-independent fluorescent indicators for glucose (Matsu-Ura et al, 2002;Ryabov et al, 1999;Woltman et al, 1999). In the case of transition metals, their fluorescence efficiency varies with the oxygen content of the sample.…”