Blood gas analyzer utility in evaluating oxygen kinetics of the aqueous humor

Abstract

Purpose: To measure the partial pressure of oxygen (PO 2 ) and carbon dioxide (PCO 2 ) and the pH of aqueous humor (AH) and arterial blood samples from rabbits using a blood gas analyzer. Methods: Twenty New Zealand rabbits were anesthetized intramuscularly with ketamine and xylazine and were then allowed to breathe room air. Using a gas blood analyzer, arterial blood and AH samples were analyzed for PO 2 , PCO 2 , and pH. Results: The mean arterial blood pressure was 87.14 ± 15.0 mmHg. The mean blood and AH PO 2 were 95.18 ± 11.76 mmHg and 88.83 ± 9.92 mmHg, the mean blood and AH PCO 2 were 25.86 ± 5.46 mmHg and 29.50 ± 5.36 mmHg, and the mean blood and AH pH were 7.38 ± 0.06 and 7.33 ± 0.09, respectively. Conclusions: The blood gas analyzer was easily employed to evaluate the aqueous humor in rabbits. When comparing the results of studies evaluating aqueous PO 2 , care should be taken to determine the methods used in these studies.

The impact of oxygen content on Staphylococcus epidermidis pathogenesis in ocular infection based on clinical characteristics, transcriptome and metabolome analysis

“…Bacteria transfer from the ocular surface to the intraocular is faced with changes in many environmental factors, such as pH, oxygen concentration, immune response, availability of nutrients, etc. ( Ersan et al, 2015 ; Agujetas et al, 2018 ; Braunger et al, 2023 ), and one change in oxygen concentration is just one of the environmental factors, this condition is not fully representative of the complex ocular surface and intraocular environment. Recent studies also suggest that microaerobic lifestyles are important in host-associated microbial communities and the prevalence of microoxygen environments ( Morris and Schmidt, 2013 ; Pedroza-Dávila et al, 2020 ), so we selected changes in environmental oxygen concentration as the experimental variable.…”

Intracameral tenecteplase during phacoemulsification in rabbits: clinical assessment of the anterior segment and biochemical analysis of the aqueous humor

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