Blood COX-2 and PGES gene transcription during the peripartum period of dairy cows with normal puerperium or with uterine infection

Silva, E.; Gaivão, Maria; Leitão, Sara; Amaro, Ana; Lopes-da-Costa, Luís; Mateus, L.

doi:10.1016/j.domaniend.2008.07.001

Cited by 20 publications

(13 citation statements)

References 45 publications

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“…Upon a pathogenic stimulus such as LPS administration, the innate immune system elicits several reactions with the intent of controlling or eliminating the infection (Abbas and Lichtman, 2007). These include synthesis of proinflammatory cytokines such as TNFα from leukocytes and subsequent increase in body temperature via COX2 modulation and hepatic synthesis of acute phase proteins including haptoglobin (Carroll and Forsberg, 2007;Silva et al, 2008). Accordingly, rectal temperature increased (P ≤ 0.02) from 2 to 6 h, plasma haptoglobin concentrations increased (P ≤ 0.05) from 16 to 96 h, and serum TNFα concentrations and blood mRNA expression TNFα and COX2 were increased (P < 0.01) at 2 h relative to LPS administration across all treatments (Table 3).…”

Section: Methodsmentioning

confidence: 99%

“…Responses were quantified on the basis of the threshold cycle (C T ), the number of PCR cycles required for target amplification to reach a predetermined threshold. The CT responses from TNFα and COX2 were normalized to β2-microglobulin (Silva et al, 2008), and the CV for β2microglobulin CT values across all samples was 4.2%.…”

Section: β2-microglobulin Nm_173893mentioning

confidence: 99%

“…Primer sequences obtained fromSilva et al (2008) for β2-microglobulin and cyclooxygenase-2 andRiollet et al (2000) for tumor necrosis factor α.Downloaded from https://academic.oup.com/jas/article-abstract/93/10/5018/4701830 by guest on 09 June 2020…”

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confidence: 99%

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Effects of oral meloxicam administration to beef cattle receiving lipopolysaccharide administration or vaccination against respiratory pathogens1

Rodrigues

Cooke

Marques

et al. 2015

Journal of Animal Science

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This study evaluated the effects of oral meloxicam administration on metabolic, inflammatory, and acute-phase responses of beef cattle receiving a lipopolysaccharide (LPS) challenge (Exp. 1; d -1 to 6) or vaccinated against respiratory pathogens (Exp. 2; d 7 to 21). Twenty-one Angus steers ( = 11) and heifers ( = 10) were housed in individual pens on d -15 and were offered free-choice water, mineral-vitamin mix, and hay until d 21. In Exp. 1, cattle were ranked on d -1 by sex and BW and assigned to 1) oral meloxicam administration (1 mg/kg BW daily) from day -1 to 6 (MEL8), 2) oral meloxicam administration (1 mg/kg BW) on d 0 and oral lactose monohydrate administration (1 mg/kg BW) on d -1 and from d 1 to 6 (MEL1), or 3) oral lactose monohydrate administration (1 mg/kg BW daily) from d -1 to 6 (CON). On d 0, cattle received an intravenous LPS bolus (0.5 μg/kg BW) concurrently with treatment administration. Rectal temperature (RTEMP) was assessed, and blood samples were collected at -2, 0, 2, 4, 6, 8, 12, 16, 24, 36, 48, 60, 72, 96, 120, and 144 h relative to LPS administration. No treatment effects were detected ( ≥ 0.36) for RTEMP, concentrations of serum tumor necrosis factor α (TNFα), plasma haptoglobin, cortisol, insulin, and leptin, as well as blood mRNA expression of α and cyclooxygenase-2, although all variables increased ( < 0.01) across treatments after LPS administration. In Exp. 2, cattle received the same treatments that they were assigned to in Exp. 1 from d 7 to d 13 and were vaccinated against respiratory pathogens concurrently with treatment administration on d 8. Blood samples were collected, and RTEMP was assessed as in Exp. 1 in addition to 168, 240, and 336 h relative to vaccination. No treatment effects were detected ( ≥ 0.26) for RTEMP, the same plasma and serum variables evaluated in Exp. 1, and serum concentrations of antibodies against or serum titers against bovine respiratory syncytial virus, bovine herpesvirus-1, bovine viral diarrhea virus-1, and parainfluenza-3 virus. All variables increased ( < 0.01) across treatments after vaccination, except for serum TNFα and titers against bovine viral diarrhea virus-1 ( ≥ 0.40). Collectively, this study found no evidence that oral meloxicam administration, at the doses and intervals utilized herein, mitigated the metabolic, inflammatory, and acute-phase reactions elicited by LPS administration or vaccination against respiratory pathogens.

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Section: Methodsmentioning

confidence: 99%

Section: β2-microglobulin Nm_173893mentioning

confidence: 99%

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Effects of oral meloxicam administration to beef cattle receiving lipopolysaccharide administration or vaccination against respiratory pathogens1

Rodrigues

Cooke

Marques

et al. 2015

Journal of Animal Science

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“…Endotoxin activates expression of pro-inflammatory genes through the binding of the LPS-binding protein to CD14, a membrane glycophosphatidylinositol anchored protein (Lahti et al, 2000) and Toll-like receptor-4 (Silva et al, 2008;Worku and Morris, 2009) on neutrophils and macrophages. Leukocytes such as the neutrophil are recruited from blood to remove bacteria (Black, 2000).…”

Section: Introductionmentioning

confidence: 99%

Influence of Heirloom Tomato Polyphenol Extracts on Expression of Inflammation Genes in Bovine

A.¹

2012

American Journal of Animal and Veterinary Sciences

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The effect of tomato on diseases of economic importance continues to be of broad interest. To evaluate these relationships, the effect of crude extracts of Cherokee purple tomato on pro-inflammatory genes and cytokines were evaluated. Neutrophils from three Holstein Fresian cows were treated with Phosphate Buffered Saline (PBS) or E. coli O111-B4 Lipopolysaccharide (LPS). Treated cells were exposed to different concentrations of polyphenol extracts of fresh and heated Cherokee purple. Transcription of Cyclooxygenase-2 (Cox-2) and Tumor Necrosis Factor-alpha (TNF-α) was detected using real time PCR. Secretion of cytokines was evaluated using specific Enzyme-Linked Immunosorbent Assay (ELISA)'s for three pro-inflammatory genes. Transcription of Cox-2 and TNF-α in bovine neutrophils were found. No significant treatment effect of tomato polyphenols was found in the LPS treated neutrophils. TNF-α and Cox-2 expression in bovine neutrophils were modulated by polyphenol treatment. Variable concentration of polyphenol extracts had no effect on Cox-2 transcription. Gene expression analysis of TNF-α and Cox-2 mRNA showed significantly decreased transcription of Cox-2 in neutrophils exposed to polyphenol extracts (p<0.0326). Polyphenol exposure did not have an influence on induction of Cox-2 by LPS. Treatment with polyphenols decreased transcription of TNF-α at the level of 200 ng mL −1 (p<0.05). Fresh or heated polyphenol did not influence transcription of TNF-α. Significant variation was observed among cows in transcription of TNF-α (p<0.05). No significant treatment effect was observed for translation in Cox-2, TNF-α and GCSF exposed to fresh or heated polyphenol extracts. Relative secretion of the antiviral pro-inflammatory cytokine IFNr was increased compared to the control in samples exposed to heated tomato polyphenol extracts (p<0.05). Results showed that tomato polyphenols modulate expression of proinflammatory genes in bovine neutrophils and may provide avenues to boost innate immunity. To our knowledge, this is the first study to discuss the role Cherokee purple polyphenol extracts plays in innate immunity.

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“…The activity of the eicosanoid-producing enzymes cyclooxygenase (COX) and 15 lipoxygenase is increased during the transition period [1,27]. COX pathway products are related to inflammatory events and in dairy cows, the COX pathway is composed of 2 enzyme isoforms, COX-1 and COX-2 [11,7].…”

Section: Introductionmentioning

confidence: 99%

Hematological Parameters in Cows in Early Lactation Treated with Ketoprofen and their Relationship with Lipid Mobilization and Ketogenesis

Kovačević

Stojanović

Cincović

et al. 2016

Acta Scientiae. Vet.

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Background: Dairy cows are exposed to numerous hematological and biochemical changes, what is bringing cows into the state of increased metabolic activity and physiological adaptations. These adaptive processes have resulted in increased lipid mobilization and ketogenesis with increased concentration of non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHB). As a non steroidal anti-inflammatory drug, ketoprofen produces anti-inflammatory effects. The main objective of the present study was to found relationship between hematological parameters and ketoprofen administration and high lipid mobilization and ketogenesis in cows treated with ketoprofen immediately after calving. Materials, Methods & Results: Ketoprofen was used (3 mg/kg body weight) intramuscularly for three consecutive days post-partum on 15 cows of Holstein-Friesian breed. Cows of the control group (n = 15) were not treated with ketoprofen. Blood samples were collected at the day of calving, in the first and in the second week after parturition from the coccygeal vein of the both groups. Hematological parameters (erythrocytes, hemoglobin, neutrophils, lymphocytes and mean platelet volume) were determined on the automatic hematological counter. Metabolic parameters (NEFA, BHB) were determined by standard colorimetric kits using a semi-automatic biochemistry analyzer. Student's t-test was used to determine the influence of the application of ketoprofen to the hematological parameters. The data analysis was performed using SPSS, version 19.0, software package for Microsoft Windows (IBM, Armonk, NY, USA). The results of comparison with (P < 0.05) were considered to represent statistically significant differences. Difference in correlation test between NEFA and BHB and other blood parameters in experimental and control group of cows were determined by the Fischer r-to-z transformation. The research results show that there was a increased red blood cell count and a higher concentration of hemoglobin (P < 0.05), fewer neutrophils (P < 0.01), lower number of lymphocytes (P < 0.01) and fewer mean platelet volume in cows that received ketoprofen after calving than those of the control. Determined hematological parameters and values of NEFA and BHB are in correlation. The correlation coefficients were significantly lower in the experimental group of cows. The intensity of the connection between hematological parameters and value of NEFA and BHB decreases. Discussion: It is well known that dairy cows with excessive adiposity manifest a low-grade inflammation and that elevated NEFA concentrations present positive risk factors for many proinflammatory diseases. Analyzing the results in this examination, it has been noticed that the increase of NEFA and BHB after calving were significantly lower in group of cows treated with ketoprofen. Also, determined hematological parameters and values of NEFA and BHB are in correlation. The intensity of these connection decreases in ketoprofen treated cows. In current study, in cows treated with ketoprofen t...

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Blood COX-2 and PGES gene transcription during the peripartum period of dairy cows with normal puerperium or with uterine infection

Cited by 20 publications

References 45 publications

Effects of oral meloxicam administration to beef cattle receiving lipopolysaccharide administration or vaccination against respiratory pathogens1

Effects of oral meloxicam administration to beef cattle receiving lipopolysaccharide administration or vaccination against respiratory pathogens1

Influence of Heirloom Tomato Polyphenol Extracts on Expression of Inflammation Genes in Bovine

Hematological Parameters in Cows in Early Lactation Treated with Ketoprofen and their Relationship with Lipid Mobilization and Ketogenesis

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