Blood compatibility of novel water soluble hyperbranched polyglycerol-based multivalent cationic polymers and their interaction with DNA

Kainthan, Rajesh K.; Gnanamani, Muthiah; Ganguli, Munia; Ghosh, Tanay; Brooks, Donald E.; Maiti, Souvik; Kizhakkedathu, Jayachandran N.

doi:10.1016/j.biomaterials.2006.06.021

Cited by 236 publications

(179 citation statements)

References 38 publications

Supporting

Mentioning

179

Contrasting

Order By: Relevance

“…Thereby, one observable is replaced by another in the following way: (5) Here and are the respective potentials and is one of the natural variables of potential, . The list of common transformations is shown below.…”

Section: Ensembles and Potentialsmentioning

confidence: 99%

“…Due to their architecture, HBP show a lower viscosity in melt and solution compared to their linear analogue and the rich amount of various functional groups offers a tuneable solubility in different solvents. Based on these advantages of HBP different applications have been suggested, for instance possible implementations of HBP are discussed in the field of medicine [1][2][3][4][5][6][7][8], catalysis [9][10][11][12][13], membrane materials [14][15][16][17][18][19][20], chemical engineering [21][22][23][24], sensors [25][26][27][28], thermosets [29][30][31] or as rheological modifier [32,33]. Polymeric drug delivery systems offer great opportunities to effectively control the drug release in human body [34][35][36][37][38][39][40][41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phase Diagrams for Systems Containing Hyperbranched Polymers

et al. 2012

View full text Add to dashboard Cite

Abstract:Hyperbranched polymers show an outstanding potential for applications ranging from chemistry over nanotechnology to pharmacy. In order to take advantage of this potential, the underlying phase behaviour must be known. From the thermodynamic point of view, the modelling of these phase diagrams is quite challenging, because the thermodynamic properties depend on the architecture of the hyperbranched polymer as well as on the number and kind of present functional end groups. The influence of architecture can be taken into account via the lattice cluster theory (LCT) as an extension of the well-known Flory-Huggins theory. Whereas the Flory-Huggins theory is limited to linear polymer chains, the LCT can be applied to an arbitrary chain architecture. The number and the kind of functional groups can be handled via the Wertheim perturbation theory, applicable for directed forces between the functional groups and the surrounding solvent molecules. The combination of the LCT and the Wertheim theory can be established for the modelling or even prediction of the liquid-liquid equilibria (LLE) of polymer solutions in a single solvent or in a solvent mixture or polymer blends, where the polymer can have an arbitrary structure. The applied theory predicts large demixing regions for mixtures of linear polymers and hyperbranched polymers, as well as for mixtures made from two hyperbranched polymers. The introduction of empty lattice sites permits the theoretical investigation of pressure effects on phase behaviour. The calculated phase diagrams were compared with own experimental data or to experimental data taken from literature. OPEN ACCESSPolymers 2012, 4 73

show abstract

Section: Ensembles and Potentialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phase Diagrams for Systems Containing Hyperbranched Polymers

et al. 2012

View full text Add to dashboard Cite

show abstract

“…[163,[304][305][306][307][308][309][310][311][312] It is important to mention that the amine shells were attached to the PG core through carbamate linkers to induce pH-responsive cleavability of the shell fragment and consequent decomplexation of the genetic cargo.…”

Section: Scientific Goalsmentioning

confidence: 99%

siRNA transfection by dendritic core–shell nanocarriers

Fischer

Claderon

Ofek

et al. 2010

Journal of Controlled Release

View full text Add to dashboard Cite

show abstract

“…27,28 As shown in Figure 3A (a-f), the hemolytic phenomenon was only observed at high concentrations of the glycogen derivatives ($250 µg/mL), after they were incubated with the erythrocytes. The hemolytic phenomenon became more pronounced, however, in the presence of bPEI ( Figure 3A [g]), mainly because of the erythrocyte membrane disruption.…”

mentioning

confidence: 93%

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Liu¹,

Ren²,

Liu³

et al. 2014

IJN

View full text Add to dashboard Cite

Background The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector. Methods A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy. Their buffer capacity was assessed by acid–base titration in aqueous NaCl solution. Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis. The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy. Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively. pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines. In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency. Results The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized. They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI). They were able to bind and condense pDNA to form the complexes of 100–250 nm in size. The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI. Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group. Conclusion The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.

show abstract

Blood compatibility of novel water soluble hyperbranched polyglycerol-based multivalent cationic polymers and their interaction with DNA

Cited by 236 publications

References 38 publications

Phase Diagrams for Systems Containing Hyperbranched Polymers

Phase Diagrams for Systems Containing Hyperbranched Polymers

siRNA transfection by dendritic core–shell nanocarriers

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Contact Info

Product

Resources

About