Blood Collection from Mice and Hematological Analyses on Mouse Blood

Rathkolb, Birgit; Fuchs, Helmut; Gailus‐Durner, Valérie; Aigner, Bernhard; Wolf, Eckhard; Angelis, Martin Hrabé de

doi:10.1002/9780470942390.mo130054

Cited by 25 publications

(23 citation statements)

References 49 publications

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“…As described in a Current Protocols article by Rathkolb et al. (), deciding which blood collection method to use depends on several parameters such as: required assay volumes, allowable methods (i.e., approved), and whether the mouse must remain alive after the blood collection procedure. Survival blood draw protocols can produce enough plasma to perform a limited number of assays.…”

Section: Strategic Planningmentioning

confidence: 99%

“…Samples should be excluded from further analysis if there is evidence of poor blood draw, leading to partial activation of the blood coagulation system or macro‐ or microthrombi that could result in consumption of the plasma hemostatic factors. Some of the hallmarks of bad blood drawing, such as hemolysis, have been excellently described (Current Protocols article: Rathkolb et al., ). Here we would like to reiterate the importance of clean blood draws.…”

Section: Commentarymentioning

confidence: 99%

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Assessing Blood Clotting and Coagulation Factors in Mice

et al. 2019

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The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules have been discovered and characterized; however, many are still unknown. Genetically modified mouse technologies are providing a plethora of new mouse models for investigating the regulation of blood coagulation. Here we provide a protocol for the tail bleeding time as a primary assessment of in vivo blood coagulation, as well as in vitro methods such as the prothrombin time, activated partial thromboplastin time, and thrombin generation assay. We also provide protocols for the assessment of the activities of specific known factors involved in blood coagulation. © 2019 by John Wiley & Sons, Inc.

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Section: Strategic Planningmentioning

confidence: 99%

Section: Commentarymentioning

confidence: 99%

Assessing Blood Clotting and Coagulation Factors in Mice

et al. 2019

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“…Additional reagents and equipment for blood collection from mice (Rathkolb et al, 2013) and preparation of plasma (Basic Protocol 2) or serum (Basic Protocol 1) Sample preparation 1. Collect blood samples into plain, heparinized, and/or EDTA-coated tubes (Rathkolb et al, 2013), mix gently by inverting the tube several times, and prepare plasma (Basic Protocol 2) or serum (Basic Protocol 1) as recommended for the parameters to be measured (see Tables 1 to 3).…”

Section: Clinicalmentioning

confidence: 99%

“…Mouse Biol. 3:69-100 C 2013 by John Wiley & Sons, Inc.Keywords: mouse r clinical chemistry r steroids r osteocalcin r PINP r ELISA r mass spectrometry Protocols for blood sample collection can be found in our contribution about hematological analyses on mouse blood (Rathkolb et al, 2013). In Basic Protocols 1 and 2, we describe the steps necessary for plasma or serum preparation and the considerations that have to be taken into account for the selection of a suitable method for sample processing.…”

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confidence: 99%

Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

et al. 2013

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Besides hematological analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined from mouse blood samples. For most of these tests, plasma or serum samples are used. Data obtained by these investigations provide indications of genotype effects on metabolism and organ functions. Here we describe in detail the considerations that have to be taken into account to get adequate samples for plasma or serum analyses and the recommended sample processing for different investigations. Furthermore, we describe established methods used in the German Mouse Clinic (GMC) to determine clinical chemical parameters; for more in-depth analysis of specific classes of biomarkers, we provide instructions for ELISAs (sandwich and competitive) as well as LC-MS/MS, focusing on markers associated with bone or steroid metabolism in the mouse as working examples. Curr. Protoc. Mouse Biol. 3:69-100 © 2013 by John Wiley & Sons, Inc.

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“…Instead, the saphenous vein is a good source from which blood can be repeatedly obtained (Rathkolb et al, 2011b). …”

Section: Basic Protocol 1: Treatment Of Mecp2-mutant Mouse Models Witmentioning

confidence: 99%

Evaluation of Rett Syndrome Symptom Improvement by Metabolic Modulators in Mecp2‐Mutant Mice

et al. 2013

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Mouse models recapitulate many symptoms of Rett Syndrome, an X-linked disorder caused by mutations in methyl-CpG-binding protein 2 (MECP2). The study of Mecp2-null male mice has provided insight into pathogenesis of the disorder; most recently, dysregulation of cholesterol and lipid metabolism. Perisymptomatic treatment with statin drugs successfully mitigates the effects of this metabolic syndrome, increases longevity and improves motor function. Described here is a metabolic drug screening protocol and timeline for symptom evaluation in Mecp2-mutant mice. Specifically, mice are treated twice weekly with a compound of interest alongside subjective health assessments, bi-weekly body composition measurements and blood chemistries. Throughout treatment, behavioral phenotyping tests are carried out at specific time points. This protocol is highly adaptable to other neurological diseases; however, the time for completion depends on the specific mutant model under study. The protocol highlights the use of several different CPMo protocols to carry out testing in a preclinical model.

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Blood Collection from Mice and Hematological Analyses on Mouse Blood

Cited by 25 publications

References 49 publications

Assessing Blood Clotting and Coagulation Factors in Mice

Assessing Blood Clotting and Coagulation Factors in Mice

Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

Evaluation of Rett Syndrome Symptom Improvement by Metabolic Modulators in Mecp2‐Mutant Mice

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