Blood cells and plasma proteins of chickens fed a diet supplemented with (1 ? 3),(1 ? 6)‐β‐D‐Glucan and enrofloxacin

Abstract

The effects of (1-->3),(1-->6)-beta-D-glucan from Saccharomyces cerevisiae and of the fluochinolone enrofloxacin were studied on red and white blood cells and plasma proteins of growing chickens up to the 35th day of life. The prominent findings within the leukocyte population on a per cent scale are: (i) increase of leukocyte count; increase of neutrophils and decrease of lymphocytes in the control and in the antibiotic group from day 17 to day 35; (ii) a minor decrease of neutrophils and no change of lymphocytes in the glucan group; (iii) the monocytes increase from 2.5 +/- 1.8% to 6.5 +/- 7.6% in the glucan group; (iv) the basophils increase in the control group and scale down in the other groups from day 17 to day 35. The total count of leukocytes increases in the controls and in the glucan group. The total protein content of blood plasma, beta-globulin and gamma-globulin increase and the albumin-globulin-ratio and alpha-globulin decline during chickens growth. These changes are most prominent in the glucan group. The haemoglobin concentration shows in all three dietary groups a highly significant increase from day 17 to day 35 by about 17 to 27 per cent; no changes are seen in packed cell volume and number of erythrocytes per litre blood.

Immunomodulating effect of B-glucans and mannan oligosaccharide on broiler chicks vaccinated with Newcastle disease virus

“…It was observed that the source of increased in TLC is the significantly increased lymphocyte counts due to use of Alphamune®, Increased TLC in group 4 can be attributed to immunostimulation effect of compounds of Alphamune. This result was previously observed by (Fleischer et al, 2000;Acevedo et al, 2001) who recoded increased TLC with administration of MOS and b-glucan respectively. Chicken group 4 that fed on Alphamune® supplemented ration and vaccinated with NDV vaccine showed significant lower AST and ALT levels at 35 days of age (Table 8, 9).…”

The influence of antibiotics on B-cell number, percentage, and distribution in the bursa of Fabricius of newly hatched chicks

“…However, enrofloxacin may lead to an increase in the bactericidal action of phagocytes, such as neutrophils, in pigs (Schoevers et al, 1999). It has also been shown that after enrofloxacin administration the number of lymphocytes in the peripheral blood of 17-d-old chickens was significantly higher than that in the control group (Fleischer et al, 2000). Moreover, tilmicosin, florfenicol, or enrofloxacin administration reduced the humoral immune response in poultry, but had beneficial effects on the cell-mediated immune response (Khalifeh et al, 2009).…”

Immunomodulating and zootechnical effect of some bacterial components on broiler chicken vaccinated with Newcastle disease vaccine

“…Data presented in Table (2, 6 and 7) showed the results of HI titer for NDV vaccine in different chicken groups and its effect on challenge with vvNDV where group that received Inmunair® and Betapolo® showed significant higher NDV genomatric mean HI titer (6.4, 5.4) and (5.9), respectively at 35 days of age. For Betapolo® (Fleischer et al, 2000;Acevedo et al 2001) observed increased humoral response to ND with B-glucan. While Inmunair® contains LPS and inactivated cells of Propionibacterium acnes, our results agree with the observations (Flo et al, 1996).…”