Blood-Based Biomarkers for Metabolic Syndrome

O’Neill, Sadhbh; Bohl, Mette; Gregersen, Søren; Hermansen, Kjeld; O’Driscoll, Lorraine

doi:10.1016/j.tem.2016.03.012

Cited by 75 publications

(52 citation statements)

References 51 publications

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“…Clinical markers considered of beneficial metabolic nature, in our study represented by glucose/insulin, HDL-C and adiponectin (57,58), showed associations largely opposite to those established for hsCRP, BMI and GGT. Examples of this include, in case of adiponectin, the decreased galactosylation of nonfucosylated diantennaries (␤ A2F0G ϭ Ϫ0.06 Ϯ 0.01; p A2F0G ϭ 3.1 ϫ 10 Ϫ8 ), a decreased sialylation of diantennaries (␤ A2GS ϭ Ϫ0.05 Ϯ 0.01; p A2GS ϭ 2.1 ϫ 10…”

Section: N-glycans Associate With Inflammation and Metabolic Healthcontrasting

confidence: 93%

Human Plasma N-glycosylation as Analyzed by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance-MS Associates with Markers of Inflammation and Metabolic Health

Reiding

Ruhaak

et al. 2017

Molecular & Cellular Proteomics

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Glycosylation is an abundant co-and post-translational protein modification of importance to protein processing and activity. Although not template-defined, glycosylation does reflect the biological state of an organism and is a high-potential biomarker for disease and patient stratification. However, to interpret a complex but informative sample like the total plasma N-glycome, it is important to establish its baseline association with plasma protein levels and systemic processes. Thus far, large-scale studies (n >200) of the total plasma N-glycome have been performed with methods of chromatographic and electrophoretic separation, which, although being informative, are limited in resolving the structural complexity of plasma N-glycans. MS has the opportunity to contribute additional information on, among others, antennarity, sialylation, and the identity of high-mannose type species.Here, we have used matrix-assisted laser desorption/ ionization ( Glycosylation is a ubiquitous co-and post-translational protein modification of functional relevance to the processing and activity of the conjugate. Examples include quality control during protein folding, regulation of circulatory half-life, and modulation of receptor interactions by either providing the recognition motif or by affecting protein conformation (1-7). Consequentially, glycosylation has been associated with a multitude of diseases and states thereof, among which the progression and metastasis of cancer and the remission of rheumatoid arthritis (8 -11). Because the process of glycosylation is not template-defined, glycosylation integrates a large series of cellular conditions such as glycosidase/glycosyltransferase abundance and activity, endoplasmic reticulum From the ‡Center

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Section: N-glycans Associate With Inflammation and Metabolic Healthcontrasting

confidence: 93%

Human Plasma N-glycosylation as Analyzed by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance-MS Associates with Markers of Inflammation and Metabolic Health

Reiding

Ruhaak

et al. 2017

Molecular & Cellular Proteomics

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“…It is thus apparent that RUNX2, a recognized master gene for osteogenesis, sits at the intersection of multiple MSC pathways regulating commitment and differentiation toward all canonical MSC fates. MiR-204&211 have been already involved in osteoporosis [14] and obesity [23,6]. Our additional data, i.e., inhibition of chondrogenesis and hematopoietic supporting activity, coupled with promotion of adipogenesis and depression of osteogenesis, strikingly recall the clinical traits of metabolic syndrome, where obesity, osteoarthritis, osteoporosis and anaemia are all combined.…”

Section: Discussionmentioning

confidence: 53%

Effect of miR-204&211 and RUNX2 control on the fate of human mesenchymal stromal cells

et al. 2017

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--MiR-204 and 211 enforced expression in murine mesenchymal stromal cells (MSCs) has been shown to induce adipogenesis and impair osteogenesis, through RUNX2 down-modulation. This mechanism has been suggested to play a role in osteoporosis associated with obesity. However, two further fundamental MSC functions, chondrogenesis and hematopoietic supporting activity, have not yet been explored. To this end, we transduced, by a lenti-viral vector, miR-204 and 211 in a model primary human MSC line, opportunely chosen among our MSC collection for displaying all properties of canonical bone marrow MSCs, except adipogenesis. Enforced expression of miR-204&211 in these cells, rescued adipogenesis, and inhibited osteogenesis, as previously reported in murine MSCs, but, surprisingly, also damaged cartilage formation and hematopoietic supporting activity, which were never explored before. RUNX2 has been previously indicated as the target of miR-204&211, whose down modulation is responsible for the switch from osteogenesis to adipogenesis. However, the additional disruption of chondrogenesis and hematopoietic supporting activity, which we report here, might depend on diverse miR-204&211 targets. To investigate this hypothesis, permanent RUNX2 knock-down was performed. Sh-RUNX2 fully reproduced the phenotypes induced by miR-204&211, confirming that RUNX2 down modulation is the major event leading to the reported functional modification on our MSCs. It seems thus apparent that RUNX2, a recognized master gene for osteogenesis, might rule all four MSC commitment and differentiation processes. Hence, the formerly reported role of miR204&211 and RUNX2 in osteoporosis and obesity, coupled with our novel observation showing inhibition of cartilage differentiation and hematopoietic support, strikingly resemble the clinical traits of metabolic syndrome, where osteoarthritis, osteoporosis, anaemia and obesity occur together. Our observations, corroborating and extending previous observations, suggest that miR-204&211-RUNX2 axis in human MSCs is possibly involved in the pathogenesis of this rapidly growing disease in industrialized countries, for possible therapeutic intervention to regenerate former homeostasis.

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“…Bloom et al (2014) confirmed that nutrition is a major modulator of these diseases and demonstrated that NCDs occurred progressively and silently during lifespan. Some health parameters; BMI, FBG, TChol, TG and Hb are used as biomarkers and help in NCDs prediction (O'Neill et al, 2016). These biomarkers vary during age from childhood to adulthood.…”

Section: Discussionmentioning

confidence: 99%

Metabolic syndrome within freshman students; Case study in Jazan Kingdom of Saudi Arabia (KSA)

Mahmoud¹,

El-Blooni²,

Abdalla³

et al. 2017

Int. J. Nutr. Metab.

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As well as many countries, in Kingdom of Saudi Arabia (KSA), non-communicable diseases are becoming an epidemic health problem. Scientific studies prove that these diseases are related to the nutritional types adopted in the last decades and which could be predicted by control of metabolic biomarkers during lifespan. Saudi Arabia has experienced marked nutritional changes and rapid urbanization in recent decades and in parallel incidence of health problems such as diabetes, obesity, hypertension, and cancers increases. This research was carried out to study the influence of canteen food on some metabolic biomarkers within freshman students in Jazan. For that, students were asked to answer questionnaires about their consumption from the canteen, socio and economic status, frequency and quality of their meals and some daily habits. Also, some metabolic biomarkers; body mass index (BMI); total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), total protein (TP), albumin (Alb), triglyceride (TG), fasting blood glucose (FBG), uric acid (UA) and hemoglobin (Hb) levels were checked twice, at the first week of the first semester then again 14 weeks after. The results showed that these students eat regularly French fries, pizza and pasta. Sweets were consumed frequently and at high rates. However, vegetables and fruits were rarely consumed. For metabolic biomarkers, BMI and LD increased significantly. However, FBG, Alb, TP and HDL decreased significantly. As conclusion, our results proved that during freshman year, students' health could be influenced and some health biomarkers could be changed.

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Blood-Based Biomarkers for Metabolic Syndrome

Cited by 75 publications

References 51 publications

Human Plasma N-glycosylation as Analyzed by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance-MS Associates with Markers of Inflammation and Metabolic Health

Human Plasma N-glycosylation as Analyzed by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance-MS Associates with Markers of Inflammation and Metabolic Health

Effect of miR-204&211 and RUNX2 control on the fate of human mesenchymal stromal cells

Metabolic syndrome within freshman students; Case study in Jazan Kingdom of Saudi Arabia (KSA)

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