Blood at 70: its roots in the history of hematology and its birth

Coller, Barry S.

doi:10.1182/blood-2015-09-659581

Cited by 23 publications

(14 citation statements)

References 80 publications

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“…The hematological parameter is a virtual reference for inflammation, and it is widely used in immunology, medical genetics, oncology, blood banking, transfusion medicine, clinical pathology, and laboratory medicine (Coller, 2015). Our hematological results indicated that WBCs, red blood cells, hemoglobin and hematocrit all exhibited obvious changes in the Ca group compared with the WT group.…”

Section: Resultsmentioning

confidence: 99%

Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model

Chen

Tian

et al. 2019

Front. Microbiol.

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Vulvovaginal candidiasis is a common fungal infection afflicting women which is primarily caused by the yeast Candida albicans ( C. albicans ). It is imperative to introduce new drug classes to counter this threat due to the continuous emergence of drug-resistant cases in recent years. The purpose of this study was to clarify the in vivo antifungal activity of perillaldehyde (PAE) against C. albicans and to prove that PAE is a promising candidate for the control of vaginal candidiasis. An animal model of vaginitis was developed to demonstrate the therapeutic and preventive effects of PAE on vaginal candidiasis, and these were evaluated through fungal and histopathological examinations. In clarifying the mechanism of PAE, standard hematological test results indicated that white blood cells (WBC) were elevated abnormally in mice infected with C. albicans , whereas when the mice were treated with various concentrations of PAE, the number of WBC in the blood was reduced. Flow cytometry was used to detect the populations of neutrophils, macrophages and CD4 T cells in the vaginal tissue of the mice. PAE was found to reduce these immune cells, which all play a key role in the inflammatory response, and the related interleukin and pro-inflammatory cytokines, including IL-17, IL-22 and TNF-α. These were detected using ELISA. Finally, we detected the expression levels of E-cadherin in the PAE treatment mouse group and discovered that it had recovered to its normal levels, but in the infection mouse group, the E-cadherin expression was clearly suppressed by the presence of C. albicans . Our data demonstrated that PAE targets these cytokines and possesses the ability to fight the fungal infection while also reducing the levels of the inflammatory factors identified. Our results demonstrated that PAE has a significant preventative and therapeutic effect on vaginal candidiasis and is a potential candidate for the treatment of vaginal Candida infections.

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Section: Resultsmentioning

confidence: 99%

Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model

Chen

Tian

et al. 2019

Front. Microbiol.

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“…It was the astute clinical observations of Richard Cabot, William Osler, and others that brought the picture of the syndromic disease with its classical triad of associated jaundice, glossitis, and myeloneuropathy into sharper focus, as nicely recorded in William Castle's historical review of the disease. 1 Coller, 2 in his commentary to mark the 70th anniversary of Blood, wrote: "The most dramatic and far reaching event in hematology in the United States in the pre-Blood period was Minot and Murphy's 1926 report that feeding liver to patients with pernicious anemia could cure this otherwise fatal disorder. This dramatic breakthrough was an enormous stimulus to hematologic investigation."…”

Section: Introductionmentioning

confidence: 99%

Vitamin B12 deficiency from the perspective of a practicing hematologist

Green

2017

Blood

244

197

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B12 deficiency is the leading cause of megaloblastic anemia, and although more common in the elderly, can occur at any age. Clinical disease caused by B12 deficiency usually connotes severe deficiency, resulting from a failure of the gastric or ileal phase of physiological B12 absorption, best exemplified by the autoimmune disease pernicious anemia. There are many other causes of B12 deficiency, which range from severe to mild. Mild deficiency usually results from failure to render food B12 bioavailable or from dietary inadequacy. Although rarely resulting in megaloblastic anemia, mild deficiency may be associated with neurocognitive and other consequences. B12 deficiency is best diagnosed using a combination of tests because none alone is completely reliable. The features of B12 deficiency are variable and may be atypical. Timely diagnosis is important, and treatment is gratifying. Failure to diagnose B12 deficiency can have dire consequences, usually neurological. This review is written from the perspective of a practicing hematologist.

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“…DLGAP1 impact on FMRP protein expression and on HSF1 factor activation led to increased invasiveness of PanNET and pancreatic ductal adenocarcinoma cancer cells [48]. Both FMRP and HSF1 were previously indicated to be upregulated in other cancers [48] and the HSF1 activity in MPN was also recently reported [49]. It is significant that a unique FMRP form was reported to be abundant in human platelets [50].…”

Section: Discussionmentioning

confidence: 99%

DLGAP1 directs megakaryocytic growth and differentiation in an MPL dependent manner in hematopoietic cells

2019

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Background The MPL protein is a major regulator of megakaryopoiesis and platelet formation as well as stem cell regulation. Aberrant MPL and downstream Jak/STAT signaling results in the development of the Myeloproliferative Neoplasms (MPN). The pathogenetic and phenotypic features of the classical MPNs cannot be explained by the known mutations and genetic variants associated with the disease. Methods In order to identify potential pathways involved in MPN development, we have performed a functional screen using retroviral insertional mutagenesis in cells dependent on MPL activation. We have used viral transduction and plasmid transfections to test the effects of candidate gene overexpression on growth and differentiation of megakaryocytic cells. The shRNA approach was used to test for the effects of candidate gene downregulation in cells. All effects were tested with candidate gene alone or in presence of hematopoietic relevant kinases in the growth medium. We assayed the candidate gene cellular localization in varying growth conditions by immunofluorescence. Flow Cytometry was used for testing of transduction efficiency and for sorting of positive cells. Results We have identified the DLGAP1 gene, a member of the Scribble cell polarity complex, as one of the most prominent positive candidates. Analyses in hematopoietic cell lines revealed DLGAP1 centrosomal and cytoplasmic localization. The centrosomal localization of DLGAP1 was cell cycle dependent and hematopoietic relevant tyrosine kinases: Jak2, SRC and MAPK as well as the CDK1 kinase promoted DLGAP1 dissociation from centrosomes. DLGAP1 negatively affected the growth rate of MPL dependent hematopoietic cells and supported megakaryocytic cells polyploidization, which was correlated with its dissociation from centrosomes. Conclusions Our data support the conclusion that DLGAP1 is a novel, potent factor in MPL signaling, affecting megakaryocytic growth and differentiation, relevant to be investigated further as a prominent candidate in MPN development. Electronic supplementary material The online version of this article (10.1186/s40364-019-0165-z) contains supplementary material, which is available to authorized users.

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Blood at 70: its roots in the history of hematology and its birth

Cited by 23 publications

References 80 publications

Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model

Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model

Vitamin B12 deficiency from the perspective of a practicing hematologist

DLGAP1 directs megakaryocytic growth and differentiation in an MPL dependent manner in hematopoietic cells

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